The Kelch/Nrf2 Antioxidant System as a Target for Some Marine Fungal Metabolites

Abstract

Marine fungal metabolites often exhibit antioxidant activity, but their effects on the Keap1/Nrf2 cellular system are rarely studied, possibly due to insufficient isolated amounts. In this work, we used a bioinformatics approach to evaluate the ability of some promising cytoprotective compounds to bind Kelch domain of Keap1 protein, and thus inhibit its interaction with Nrf2. The molecular docking data suggested that gliorosein, niveoglaucin A, 6-hydroxy-N-acetyl-β-oxotryptamine, 4-hydroxyscytalone and 4-hydroxy-6-dehydroxyscytalone can form the hydrogen building with Arg415 or Arg483 amino acid residues of P1-P2 sub-pockets in the Nrf2 binding site of Keap1′s Kelch domain. These positions of the small molecules in the Kelch domain of Keap1 can inhibit the interaction of Keap1 with Nrf2 and enhance the nuclear translocation of Nrf2 from cytosol that can result in overexpression of relative genes. This assumption, based on virtual screening of a number of low molecular weight metabolites of marine fungi, makes them promising for further studies.