Bougainvillea Flower Extract Utilization as Eosin Substitute in Worm Eggs Microscopic Observation

Abstract

Introduction: Flavonoids, glycosides, alkaloids, phlobatannins, saponins, steroids, tannins, and terpenoids are all known components of bougainvillea flowers. The betalain compounds betasianin and betaxanthin, which give bougainvillea flowers their color, are present. Betalains are water-soluble pigments classified into betacyanins (red) and betaxanthins (yellow). The eosin dye, which can stain worm eggs, is comparable to the pigment in bougainvillea flowers. Because reagents are expensive, raw materials from the local area must be used instead. Local raw materials can replace eosin reagents. When examining intestinal nematode worm eggs, the extract of bougainvillea flowers can be used as an eosin reagent. We are interested in learning whether Bougenvillea flower extract can be used to observe worm eggs at a microscopic level instead of the expensive and difficult-to-come-by eosin. Aim: This study aimed to ascertain whether Bougainvillea extract could be used instead of eosin to observe worm eggs at a microscopic level. Method: Complete Randomised Design (CRD) is used in this quasi-experimental research. This study also aims to compare the efficacy of eosin reagent and bougainvillea flower extract in the protracted drying process. The Microbiology Laboratory, Department of Medical Laboratory Technology, Aceh Health Polytechnic, Ministry of Health, was the site of this study. Gampong Jawa, Banda Aceh, is where feces were sampled. Preparation of bougainvillea flower extract with a concentration of 100% as a substitute for 2% eosin on microscopic observations of Ascaris lumbricoides, Trichuris trichiura, and Hookworm eggs to see the comparison of the drying time for reagents in worm egg preparations using red, pink and orange Bougainvillea flower extract with eosin control. Repeat three more times. T-test used for data analysis. Result: When compared to the eosin control reagent, which dried in only 27.63 minutes in preparation for Soil Transmitted Helminth worm eggs, the average drying time for the reagents using natural reagents from the extract of red bougainvillea flowers was 23.96 minutes, that of pink bougainvillea flowers was 23.30 minutes, and that of orange bougainvillea flowers was longer at 28.40 minutes. A variety of bougainvillea species have different drying requirements for regeneration. Conclusion: The time it took for red, pink, and orange Bougainvillea extract to dry, as well as the control eosin, indicates that there is a significant difference in the dry times between the treatments. Keywords: Bougainvillaea extract, eosin reagents, worm eggs