Application of the pressure chamber method to evaluate root hydraulic conductance in rice plants with tillers

IF 1 Q3 PLANT SCIENCES Plant Root Pub Date : 2023-01-01 DOI:10.3117/plantroot.17.59
Emi Kameoka, Shiro Mitsuya, Akira Yamauchi
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Abstract

The root hydraulic conductance of rice is often measured using the pressure chamber method only for the main stem at the seedling stage, as it is difficult to evaluate at a more advanced growth stage with tillers due to the high risk of pressure leakage from the gaps between the tillers. The aim of this study was to identify techniques that are effective for prevention of air leakage and an improvement in the success rate of root hydraulic conductance measurements in rice plants with tillers. Using three rice (Oryza sativa L.) genotypes, FR13A (aus), KDML105 (indica), and Swarna (indica), the root hydraulic conductance of the main stem and four tillers were calculated using the pressure chamber method at 98, 104, and 95 days after sowing, respectively, using the following three techniques: 1) careful detachment of dry leaf sheaths, 2) ensuring spacing between tillers and an appropriate amount of silicone in the socket, and 3) pre-fixing the socket to prevent vertical misalignment. Using these three techniques, we achieved a success rate of 92.8% (13 of 14 plants) for root hydraulic conductance measurements in the genotypes. Additionally, we show that genotypic variation in root hydraulic conductance exists at the late vegetative stage, and that the growth stage can have a significant effect on root hydraulic conductance values. In conclusion, this study presents a detailed pressure chamber method for measuring root hydraulic conductance in rice plants with tillers, reducing the risk of pressure leakage, and improving the success rate of the measurement.
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压力室法在水稻分蘖植株根系水力导度评价中的应用
水稻根系水力导度通常只在苗期使用压力室法测量,因为在分蘖较晚的生长阶段很难评估,因为分蘖之间的间隙有很大的压力泄漏风险。本研究的目的是确定有效防止漏风和提高水稻分蘖植株根系水力导度测量成功率的技术。以水稻(Oryza sativa L.) FR13A (aus)、KDML105(籼稻)和Swarna(籼稻)3个基因型为研究材料,分别在播种后98、104和95 d采用压力室法计算了主茎和4个分蘖的根系水力导度,采用以下3种技术:1)小心分离干叶鞘,2)确保分蘖与插孔中适量的硅酮间距,3)预先固定插孔以防止垂直不对准。使用这三种技术,14株植物中有13株的根系水力导度测量成功率为92.8%。此外,我们发现在营养后期根系水导度存在基因型变异,并且生长阶段对根系水导度值有显著影响。综上所述,本研究提出了一种详细的压力室测量水稻分蘖植株根系水力导度的方法,降低了压力泄漏的风险,提高了测量成功率。
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来源期刊
Plant Root
Plant Root PLANT SCIENCES-
CiteScore
1.50
自引率
0.00%
发文量
2
期刊介绍: Plant Root publishes original papers, either theoretical or experimental, that provide novel insights into plant roots. The Journal’s subjects include, but are not restricted to, anatomy and morphology, cellular and molecular biology, biochemistry, physiology, interactions with soil, mineral nutrients, water, symbionts and pathogens, food culture, together with ecological, genetic and methodological aspects related to plant roots and rhizosphere. Work at any scale, from the molecular to the community level, is welcomed.
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