{"title":"Plant growth-enhancing traits of rhizobacteria isolated from brinjal, okra, and leaf mustard","authors":"A. H. Umar, F. A. Zakry","doi":"10.3117/plantroot.17.1","DOIUrl":"https://doi.org/10.3117/plantroot.17.1","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of a method for high-throughput quantitation of soil-surface roots of rice (Oryza sativa) and wild rice (O. glumaepatula) using an overhead scanner","authors":"Tomoki Miyashita, K. Shiono","doi":"10.3117/plantroot.17.16","DOIUrl":"https://doi.org/10.3117/plantroot.17.16","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"76 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Strontium-induced mineral imbalance, cell death, and reactive oxygen species generation in Arabidopsis thaliana","authors":"Takeshi Nagata","doi":"10.3117/plantroot.17.36","DOIUrl":"https://doi.org/10.3117/plantroot.17.36","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Acidic soil tolerance of sugarcane and Erianthus root assessed by cell membrane stability","authors":"H. Takaragawa, H. Matsuda, Y. Terajima","doi":"10.3117/plantroot.17.26","DOIUrl":"https://doi.org/10.3117/plantroot.17.26","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"5 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The root hydraulic conductance of rice is often measured using the pressure chamber method only for the main stem at the seedling stage, as it is difficult to evaluate at a more advanced growth stage with tillers due to the high risk of pressure leakage from the gaps between the tillers. The aim of this study was to identify techniques that are effective for prevention of air leakage and an improvement in the success rate of root hydraulic conductance measurements in rice plants with tillers. Using three rice (Oryza sativa L.) genotypes, FR13A (aus), KDML105 (indica), and Swarna (indica), the root hydraulic conductance of the main stem and four tillers were calculated using the pressure chamber method at 98, 104, and 95 days after sowing, respectively, using the following three techniques: 1) careful detachment of dry leaf sheaths, 2) ensuring spacing between tillers and an appropriate amount of silicone in the socket, and 3) pre-fixing the socket to prevent vertical misalignment. Using these three techniques, we achieved a success rate of 92.8% (13 of 14 plants) for root hydraulic conductance measurements in the genotypes. Additionally, we show that genotypic variation in root hydraulic conductance exists at the late vegetative stage, and that the growth stage can have a significant effect on root hydraulic conductance values. In conclusion, this study presents a detailed pressure chamber method for measuring root hydraulic conductance in rice plants with tillers, reducing the risk of pressure leakage, and improving the success rate of the measurement.
水稻根系水力导度通常只在苗期使用压力室法测量，因为在分蘖较晚的生长阶段很难评估，因为分蘖之间的间隙有很大的压力泄漏风险。本研究的目的是确定有效防止漏风和提高水稻分蘖植株根系水力导度测量成功率的技术。以水稻(Oryza sativa L.) FR13A (aus)、KDML105(籼稻)和Swarna(籼稻)3个基因型为研究材料，分别在播种后98、104和95 d采用压力室法计算了主茎和4个分蘖的根系水力导度，采用以下3种技术:1)小心分离干叶鞘，2)确保分蘖与插孔中适量的硅酮间距，3)预先固定插孔以防止垂直不对准。使用这三种技术，14株植物中有13株的根系水力导度测量成功率为92.8%。此外，我们发现在营养后期根系水导度存在基因型变异，并且生长阶段对根系水导度值有显著影响。综上所述，本研究提出了一种详细的压力室测量水稻分蘖植株根系水力导度的方法，降低了压力泄漏的风险，提高了测量成功率。
{"title":"Application of the pressure chamber method to evaluate root hydraulic conductance in rice plants with tillers","authors":"Emi Kameoka, Shiro Mitsuya, Akira Yamauchi","doi":"10.3117/plantroot.17.59","DOIUrl":"https://doi.org/10.3117/plantroot.17.59","url":null,"abstract":"The root hydraulic conductance of rice is often measured using the pressure chamber method only for the main stem at the seedling stage, as it is difficult to evaluate at a more advanced growth stage with tillers due to the high risk of pressure leakage from the gaps between the tillers. The aim of this study was to identify techniques that are effective for prevention of air leakage and an improvement in the success rate of root hydraulic conductance measurements in rice plants with tillers. Using three rice (Oryza sativa L.) genotypes, FR13A (aus), KDML105 (indica), and Swarna (indica), the root hydraulic conductance of the main stem and four tillers were calculated using the pressure chamber method at 98, 104, and 95 days after sowing, respectively, using the following three techniques: 1) careful detachment of dry leaf sheaths, 2) ensuring spacing between tillers and an appropriate amount of silicone in the socket, and 3) pre-fixing the socket to prevent vertical misalignment. Using these three techniques, we achieved a success rate of 92.8% (13 of 14 plants) for root hydraulic conductance measurements in the genotypes. Additionally, we show that genotypic variation in root hydraulic conductance exists at the late vegetative stage, and that the growth stage can have a significant effect on root hydraulic conductance values. In conclusion, this study presents a detailed pressure chamber method for measuring root hydraulic conductance in rice plants with tillers, reducing the risk of pressure leakage, and improving the success rate of the measurement.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135600382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Emi Kameoka, Shiro Mitsuya, R. Suralta, A. Yamauchi, A. Henry
{"title":"Genotypic variation in rice root system distribution and activity in response to short-term soil drought","authors":"Emi Kameoka, Shiro Mitsuya, R. Suralta, A. Yamauchi, A. Henry","doi":"10.3117/plantroot.17.45","DOIUrl":"https://doi.org/10.3117/plantroot.17.45","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The effects of hydrogen sulfide (H2S), released from the donor sodium hydrosulfide (NaHS), on maize seedlings grown hydroponically for 6 days were investigated. Plant biomass, malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide (O2•−) content, and root exudates (organic acids) were measured. Results showed that 100 and 200 µM NaHS is the most appropriate and suitable concentration for the growth and development of maize seedlings, without affecting the MDA and H2O2 contents but altering the O2•−. In addition, high concentrations of 500 and 1000 µM NaHS adversely affected these parameters compared with the control (CK). The pH of the root exudates declined under NaHS treatments. The organic acids in the root exudates, including fumaric, acetic, formic, and malic acids exhibited higher contents at 100 µM NaHS treatment, the lactic and citric acids were higher at both 100 and 200 µM NaHS. In contrast, oxalic acid was reduced at all NaHS concentrations compared with the CK. Low contents of all the organic acids analyzed were found under 500 and 1000 µM NaHS treatment. In conclusion, all the above parameters were affected by the application of NaHS, while higher NaHS concentration was toxic for maize seedlings.
{"title":"Physiological, biochemical and root exudate response of maize seedlings to hydrogen sulfide applications","authors":"Kabir Ghoto, Gui-Feng Gao, Martin Simon, Zhi-Jun Shen, Huan Li, Ming-Yue Wei, Hai-Lei Zheng","doi":"10.3117/plantroot.17.71","DOIUrl":"https://doi.org/10.3117/plantroot.17.71","url":null,"abstract":"The effects of hydrogen sulfide (H2S), released from the donor sodium hydrosulfide (NaHS), on maize seedlings grown hydroponically for 6 days were investigated. Plant biomass, malondialdehyde (MDA), hydrogen peroxide (H2O2), superoxide (O2•−) content, and root exudates (organic acids) were measured. Results showed that 100 and 200 µM NaHS is the most appropriate and suitable concentration for the growth and development of maize seedlings, without affecting the MDA and H2O2 contents but altering the O2•−. In addition, high concentrations of 500 and 1000 µM NaHS adversely affected these parameters compared with the control (CK). The pH of the root exudates declined under NaHS treatments. The organic acids in the root exudates, including fumaric, acetic, formic, and malic acids exhibited higher contents at 100 µM NaHS treatment, the lactic and citric acids were higher at both 100 and 200 µM NaHS. In contrast, oxalic acid was reduced at all NaHS concentrations compared with the CK. Low contents of all the organic acids analyzed were found under 500 and 1000 µM NaHS treatment. In conclusion, all the above parameters were affected by the application of NaHS, while higher NaHS concentration was toxic for maize seedlings.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"121 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135600389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Y. Nakano, Junpei Konishi, Hirotake Ito, Tsuyoshi Tanaka, M. Seki, H. Aoki, T. Nagamine
: The root growth angle (RGA) is an important breeding target that confers high crop adaptability to deleterious environments. In barley, natural variations in RGA among accessions have been observed, but many of the genetic factors that cause of this variation remains unclear. In this study, we explored the orthologs of OsDRO1 ( DEEPER ROOTING 1 ) and OsqSOR1 (quantitative trait locus for SOIL SURFACE ROOTING 1) , which play a critical role in RGA regulation in rice, from barley genome and analyzed the polymorphisms of these genes among barley accessions. BLASTP search detected putative orthologs of OsDRO1 and OsqSOR1 in barley (HvDRO1 and HvqSOR1) with more than 60% amino acid similarity. Sequence analysis identified SNPs causing mis-splicing and nonsynonymous amino acid substitution in HvDRO1 and HvqSOR1 , respectively. These SNPs were associated with RGA variation among the 47 barley accessions. Phylogenetic analysis using the 105 barley accessions revealed that the alleles of HvDRO1 and HvqSOR1 are related to the genetic background of the accessions. Further-more, the mutant allele of HvDRO1 is mainly shared in the Hokuriku/Nagano subpopulation, suggesting that the mutant allele is involved in local adaptation of barley cultivars to the soil environment of the region. Our findings suggest that the polymorphisms of HvDRO1 and HvqSOR1 are possible determinants of RGA variation in barley, at least in Japanese accessions, and provide information on allelic variants of the genes for marker-assisted selection to genetic improvement of RGA of barley.
{"title":"Polymorphism of HvDRO1 and HvqSOR1 associated with root growth angle in barley accessions","authors":"Y. Nakano, Junpei Konishi, Hirotake Ito, Tsuyoshi Tanaka, M. Seki, H. Aoki, T. Nagamine","doi":"10.3117/plantroot.16.1","DOIUrl":"https://doi.org/10.3117/plantroot.16.1","url":null,"abstract":": The root growth angle (RGA) is an important breeding target that confers high crop adaptability to deleterious environments. In barley, natural variations in RGA among accessions have been observed, but many of the genetic factors that cause of this variation remains unclear. In this study, we explored the orthologs of OsDRO1 ( DEEPER ROOTING 1 ) and OsqSOR1 (quantitative trait locus for SOIL SURFACE ROOTING 1) , which play a critical role in RGA regulation in rice, from barley genome and analyzed the polymorphisms of these genes among barley accessions. BLASTP search detected putative orthologs of OsDRO1 and OsqSOR1 in barley (HvDRO1 and HvqSOR1) with more than 60% amino acid similarity. Sequence analysis identified SNPs causing mis-splicing and nonsynonymous amino acid substitution in HvDRO1 and HvqSOR1 , respectively. These SNPs were associated with RGA variation among the 47 barley accessions. Phylogenetic analysis using the 105 barley accessions revealed that the alleles of HvDRO1 and HvqSOR1 are related to the genetic background of the accessions. Further-more, the mutant allele of HvDRO1 is mainly shared in the Hokuriku/Nagano subpopulation, suggesting that the mutant allele is involved in local adaptation of barley cultivars to the soil environment of the region. Our findings suggest that the polymorphisms of HvDRO1 and HvqSOR1 are possible determinants of RGA variation in barley, at least in Japanese accessions, and provide information on allelic variants of the genes for marker-assisted selection to genetic improvement of RGA of barley.","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69621664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuki Akamatsu, R. Tajima, T. Uno, Toyoaki Ito, M. Nishida, M. Saito
{"title":"Characterization of root traits for phosphorus deficiency tolerance using chromosome segment substitution lines","authors":"Yuki Akamatsu, R. Tajima, T. Uno, Toyoaki Ito, M. Nishida, M. Saito","doi":"10.3117/plantroot.16.21","DOIUrl":"https://doi.org/10.3117/plantroot.16.21","url":null,"abstract":"","PeriodicalId":20205,"journal":{"name":"Plant Root","volume":"1 1","pages":""},"PeriodicalIF":0.6,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69634224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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