Isolation of kerosene-degrading bacteria from soil samples and determination of optimal growth conditions

Abstract

Bacteria capable of decomposing hydrocarbon rocket power kerosene T-1 were isolated and studied. In the course of the studies, 30 isolates of microbial cultures were isolated from soils contaminated with hydrocarbon rocket fuel, of which 9 isolates were selected that actively assimilate kerosene T-1 as the only headwater of carbon. The strains that showed the best results in these screening analyses on four nutrient media with a concentration of T-1 kerosene 1% (10 g/kg) grow well 9 isolates of culture microorganisms: (cid:0) 4, 8, 14, 23, 5, 18, 20, 25 and yeast (cid:0) 12/5. Isolates on a medium with a concentration of T-1 kerosene 2% (20 g/kg) and 5% (50 g/kg) showed good growth in bacterial cultures (cid:0) 5, 18, 20, 25, and yeast (cid:0) 12/5. The selected microorganisms were identified by physiological and biochemical characteristics: (cid:0) 5 – Arthrobacter sp., (cid:0)18 – Acinetobacter calcoaceticum, (cid:0)20 – Bacillus sp., (cid:0)25-Micrococcus roseus, (cid:0) 12/5-Candida sp. Cultivation conditions for isolated microorganisms were created. It has been established that the optimal development temperature for the culture of Arthrobacter sp. 5 is 25-30°C, Acinetobacter calcoaceticum. 18 is 30-35 °(cid:0) , Micrococcus roseus. 25 is 25-37 °(cid:0). The optimal duration of cultivation of Candida sp. 12/5 is 1 day, for the rest of the studied cultures - 2 days.