Insecticide Resistance Development and Detoxification Enzyme Activities of Spodoptera litura (Fabricius) in Soybean from Kumaon Himalayas

IF 0.8 4区 生物学 Q3 AGRONOMY Legume Research Pub Date : 2023-05-30 DOI:10.18805/lr-5101
Rashmi Joshi, Neeta Gaur, Sudha Mathpal
{"title":"Insecticide Resistance Development and Detoxification Enzyme Activities of Spodoptera litura (Fabricius) in Soybean from Kumaon Himalayas","authors":"Rashmi Joshi, Neeta Gaur, Sudha Mathpal","doi":"10.18805/lr-5101","DOIUrl":null,"url":null,"abstract":"Background: Soybean is an important crop of Uttarakhand both in hills as well as in plains and Spodoptera litura is an important pest of soybean in Uttarakhand. Several insecticides have been in use for its management and resistance has been observed against different group of insecticides. Thus, to study the level of infestation and insecticide resistance from soybean fields of hill farmers of Kumaon Himalaya, survey and experiments were conducted. Methods: Survey was conducted and scale of infestation was determined. Chemical and biochemical assay were conducted to study insecticide resistance. For chemical assay IRAC Method No. 7 using insecticides indoxacarb 14.5% SC, chlorantraniliprole 18.5% Sc, fipronil 5% SC, chlorpyriphos 50%+cypermethrin 5% EC and profenofos 40%+cypermethrin 4% EC and for biochemical assay the specific activity of Carboxylesterase, Acetyl choline esterase, Mono-oxygenase and Glutathione-S-transferase was studied. Result: The infestation level from different regions showed the presence of S. litura, ranging from mild to severe infestation. The resistance ratio of different insecticides used ranged from a low to an extremely high resistance level with LC50 ranging from 0.37 µg/ml to 1648.84 µg/ml. The biochemical analysis of carboxylesterase, acetylcholinesterase, monooxygenase and gluthione-s-transferase showed an overproduction of these detoxification enzymes in the resistant population.","PeriodicalId":18181,"journal":{"name":"Legume Research","volume":null,"pages":null},"PeriodicalIF":0.8000,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Legume Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18805/lr-5101","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Soybean is an important crop of Uttarakhand both in hills as well as in plains and Spodoptera litura is an important pest of soybean in Uttarakhand. Several insecticides have been in use for its management and resistance has been observed against different group of insecticides. Thus, to study the level of infestation and insecticide resistance from soybean fields of hill farmers of Kumaon Himalaya, survey and experiments were conducted. Methods: Survey was conducted and scale of infestation was determined. Chemical and biochemical assay were conducted to study insecticide resistance. For chemical assay IRAC Method No. 7 using insecticides indoxacarb 14.5% SC, chlorantraniliprole 18.5% Sc, fipronil 5% SC, chlorpyriphos 50%+cypermethrin 5% EC and profenofos 40%+cypermethrin 4% EC and for biochemical assay the specific activity of Carboxylesterase, Acetyl choline esterase, Mono-oxygenase and Glutathione-S-transferase was studied. Result: The infestation level from different regions showed the presence of S. litura, ranging from mild to severe infestation. The resistance ratio of different insecticides used ranged from a low to an extremely high resistance level with LC50 ranging from 0.37 µg/ml to 1648.84 µg/ml. The biochemical analysis of carboxylesterase, acetylcholinesterase, monooxygenase and gluthione-s-transferase showed an overproduction of these detoxification enzymes in the resistant population.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Kumaon - Himalayas大豆斜纹夜蛾(Spodoptera斜纹夜蛾)抗性发育及解毒酶活性
背景:大豆是北阿坎德邦丘陵和平原的重要作物,斜纹夜蛾是北阿坎德邦重要的大豆害虫。已使用几种杀虫剂对其进行管理,并观察到对不同种类杀虫剂的抗药性。为此,通过调查和试验,研究了库马高原山地农民大豆田的侵染程度和对杀虫剂的抗性。方法:进行调查,确定侵害规模。采用化学生化试验研究其抗药性。化学分析采用IRAC法7号,采用茚虫威14.5% SC、氯虫腈18.5% SC、氟虫腈5% SC、毒死蜱50%+高效氯氰菊酯5% EC、丙烯磷40%+高效氯氰菊酯4% EC进行,生化分析采用羧酯酶、乙酰胆碱酯酶、单加氧酶、谷胱甘肽s -转移酶的比活性。结果:不同地区均有斜纹夜蛾侵染,侵染程度由轻到重。不同杀虫剂的抗性比从低到极高,LC50为0.37µg/ml ~ 1648.84µg/ml。羧酸酯酶、乙酰胆碱酯酶、单加氧酶和谷胱甘肽-s转移酶的生化分析表明,抗性群体中这些解毒酶过量产生。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Legume Research
Legume Research AGRONOMY-
CiteScore
1.30
自引率
37.50%
发文量
256
审稿时长
6-12 weeks
期刊介绍: Legume Research An International Journal. The Journal is an official publication of Agricultural Research Communication Centre. It is designed to bring out the original research articles on genetics, breeding, physiology, bacterial avtivity, production, quality, biochemistry and seeds of legumes crops. The objective of the journal is to serve as a forum for scientific community to publish their research findings on legumes ans to provide basis for new research. Journal is being scanned in the important indexing and abstracting services.
期刊最新文献
Effect of Different Irrigation Regimes on the Yield and Qualitative Characteristics of Different Cultivars of Soybean (Glycine max L.) in Jiroft Productivity and Econometric Analysis of Leguminous Monkey-nut (Arachis hypogaea L.) as Influenced by Growth Retardant Long-term Experiment on Fennel-cluster Bean Cropping System as Affected by Production Systems: A Strategy for Improving Yield, Quality and Soil Fertility Response of IWM Strategies on Growth, Nodulation, Productivity and Profitability in Fenugreek (Trigonella foenum-graecum L.) under Haryana Conditions Influence of Morphological and Biochemical Parameters for Infection of Mungbean Yellow Mosaic Virus and Bemisia tabaci in Mungbean and Urdbean
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1