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Effect of Different Irrigation Regimes on the Yield and Qualitative Characteristics of Different Cultivars of Soybean (Glycine max L.) in Jiroft 不同灌溉制度对吉洛特不同品种大豆产量及品质的影响
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-11-07 DOI: 10.18805/lrf-761
R. Mohammad Shirazi, M.H. Shirzadi, A. Aien, G.H.R. Afsharmanesh
Background: Identification and development of new varieties are important elements of improving and sustaining soybean yield, especially in arid and semi-arid regions, including Iran. Methods: The study aims to investigate the effect of different levels of low irrigation on yield and qualitative characteristics of different soybean cultivars (Dezful 504, Sahar, Telar, Tekavar, Zan and Sari) under water stress (90%, 70% and 50% of the field capacity), under the climatic conditions of Jiroft during two years (2020 and 2021). This experiment was carried out as a split plot based on a randomized complete block design with 18 treatments and 3 replications at the farm of South Kerman Agricultural Research and Training Center and Natural Resources. Result: The highest qualitative characteristics and performance were in the cultivar 504 Dezful. Examining the results related to the interaction effect of irrigation ´ variety showed that the variety 504 Dezful under the conditions of 90% of the field capacity has the highest amount of grain yield (3748 kg/ha), oil yield (916.41 kg/ha) and protein yield (1351.53 kg/ha). Also, this cultivar was superior to cultivars in all levels of low irrigation. In general, and according to the obtained results, Dezful cultivar 504 was identified as the most resistant cultivar and Sari cultivar as the most sensitive cultivar to drought stress.
背景:新品种的鉴定和开发是提高和维持大豆产量的重要因素,特别是在干旱和半干旱地区,包括伊朗。方法:研究2020年和2021年2年吉洛特气候条件下,不同低灌溉水平对不同大豆品种(Dezful 504、Sahar、Telar、Tekavar、Zan和Sari)在水分胁迫(田间容量的90%、70%和50%)下产量和品质特性的影响。本试验采用完全随机区组设计,在南克尔曼农业研究与培训中心和自然资源农场进行18个处理、3个重复的分割区试验。结果:504dezful品种的品质特征和性能最高。对灌溉品种互作效应相关结果的检验表明,504 Dezful品种在90%田间容量条件下,籽粒产量(3748 kg/ha)、油料产量(916.41 kg/ha)和蛋白质产量(1351.53 kg/ha)最高。同时,该品种在低灌条件下的表现均优于其他品种。总的来说,根据所获得的结果,确定Dezful品种504对干旱胁迫的抗性最强,而Sari品种对干旱胁迫最敏感。
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引用次数: 0
Productivity and Econometric Analysis of Leguminous Monkey-nut (Arachis hypogaea L.) as Influenced by Growth Retardant 生长阻燃剂对豆科猴果产量的影响及计量经济分析
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-28 DOI: 10.18805/lr-5253
S. Ravi, S.R. Shri Rangasami, N. Vadivel, T. Pradeesh Kumar, K. Harishankar, S. Vishnu Shankar, R. Vigneshwaran
Background: Groundnut (Arachis hypogaea L.) holds significant importance as a staple food legume and a key economic crop, serving as a valuable source of edible oil and protein in India. However, its full yield potential is often hampered by excessive vegetative growth, which leads to suboptimal yields. With this background, a field experiment was conducted at the Perunthalaivar Kamaraj Krishi Vigyan Kendra in Puducherry during 2020 and 2021. The objective was to investigate the influence of different concentrations and timing of paclobutrazol (PBZ) application on the growth and yield of groundnut. Methods: The experiment was laid out in a split-plot design with three replications, six main plot treatments (Including PBZ concentrations of 25, 50, 100, 150, 200 ppm and a control) and three sub-plot treatments (Involving single sprays at 30 days after emergence (DAE), single sprays at 50 DAE and double sprays at 30 and 50 DAE). Result: The results revealed that the application of paclobutrazol at different concentrations had a positive effect on reducing plant height during the later stages of growth, particularly when a double spray was applied at 30 and 50 DAE. Notably, the application of paclobutrazol at a concentration of 200 ppm in groundnut cultivation proved to be economically viable, resulting 29% increase in pod yield compared to the control group. Specifically, PBZ at 200 ppm, with a double spray, significantly boosted the total pod yield to 2724 kg ha-1. Furthermore, the correlation and regression analyses indicated a positive relationship between growth and yield.
背景:花生(arachhis hypogaea L.)作为一种重要的主食、豆类和重要的经济作物,在印度具有重要的意义,是食用油和蛋白质的宝贵来源。然而,它的全部产量潜力往往受到过度营养生长的阻碍,这导致了次优产量。在此背景下,于2020年和2021年在普杜切里的Perunthalaivar Kamaraj Krishi Vigyan Kendra进行了现场试验。研究了不同浓度和施用多效唑(PBZ)对花生生长和产量的影响。方法:采用分区设计,设3个重复,6个主区处理(含PBZ浓度分别为25、50、100、150、200 ppm和对照)和3个分区处理(羽化后30 d单次喷施、50 d单次喷施、30和50 d双次喷施)。结果:不同浓度的多效唑在生长后期对降低株高有积极作用,特别是在30和50 DAE时双喷。值得注意的是,花生栽培中施用浓度为200 ppm的多效唑在经济上是可行的,与对照组相比,豆荚产量增加了29%。具体而言,在200 ppm的浓度下,双喷PBZ显著提高了豆荚总产量,达到2724 kg ha-1。此外,相关分析和回归分析表明,生长与产量呈正相关。
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引用次数: 0
Long-term Experiment on Fennel-cluster Bean Cropping System as Affected by Production Systems: A Strategy for Improving Yield, Quality and Soil Fertility 生产制度影响下茴香-丛豆种植制度的长期试验:提高产量、品质和土壤肥力的策略
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-28 DOI: 10.18805/lr-5186
S. Lal, G. Lal, N.K. Meena, C.K. Jangir, M.K. Choudhary, N. Chaudhary, R.D. Meena, S.S. Meena, M.D. Meena, O.P. Aishwath
Background: The rise in unprecedented pressure of producing enough food for an ever-growing human population resulted in the use of considerable amounts of agrochemicals in crop production. To overcome this, inclusion of annual pulse crops such as cluster beans (Cyamopsis tetragonoloba L.) in cropping systems is an alternative technique for enhancing output while minimising environmental impact. Therefore, to assess the influence of different crop production systems on yield, quality and soil fertility attributes of fennel (Foeniculum vulgare L.)-cluster bean (Cyamopsis tetragonoloba L.) crop sequence, a long term field experiment was conducted. Methods: A field experiment was conducted on fennel (Foeniculum vulgare L.) cluster bean (Cyamopsis tetragonoloba L.) crop sequence under AI-NPOF Project during 2016-17 to 2020-21 at ICAR-National Research Centre on Seed Spices, Ajmer (Rajasthan), India. The experiment was laid out as a factorial design based on randomized complete blocks (RCBD) with three replications. The first factor consisted of six production systems viz., (PS1) - 100% organic, (PS2) - 75% organic + 25% innovative practice (Compost extract, cattle urine), (PS3) - Integrated (50% organic + 50% inorganic), (PS4) - Integrated (75% organic + 25% inorganic), (PS5) -100% inorganic nutrient sources and (PS6) - State recommendation] including fennel (Rabi season) - cluster bean (Kharif season) cropping sequence, whereas the second factor included the environment over the period. Result: The outcomes of study revealed that production system (PS4) - Integrated (75% organic + 25% inorganic) resulted in the maximum seed yields and yield attributes of cluster bean (1501.87 kg ha-1) and fennel (1970.19 kg ha-1). The study shows that the production system (PS4) had a 17.29 and 30.75% increment of protein as compared to PS5 and PS1 in fennel and cluster beans, respectively. Under production system PS1, the per cent increment of soil organic carbon (~25.2%) and readily available N (~9%), P (~38.5%) and K (~Non-significant) were noted over the years respectively, compared with production system (PS5). A significant positive association was observed between fennel and cluster bean grain yield and soil organic carbon (P less than 0.01) and the Shannon-Wiener indexes (R2 = 0.75, R2 = 0.69), respectively. The combined assessment of yield, quality, soil organic carbon and soil sustainability shows that rotations based on legume crops performed better and provided a model of sustainable crop intensification. Thus, adding legume to the cropping system and implementing integrated nutrient management (INM) could gradually improve soil quality in agro-ecologies where fennel predominates.
背景:为不断增长的人口生产足够的粮食的空前压力的增加导致在作物生产中使用大量农用化学品。为了克服这一问题,在种植系统中加入一年生脉冲作物,如集束豆(Cyamopsis tetragonoloba L.),是一种提高产量同时将环境影响降至最低的替代技术。因此,为了评估不同作物生产制度对茴香-四角豆作物序列产量、品质和土壤肥力属性的影响,进行了长期大田试验。方法:在AI-NPOF项目下,于2016- 2017年至2020- 2021年在印度拉贾斯坦邦的icar国家种子香料研究中心对茴香(Foeniculum vulgare L.)集束豆(Cyamopsis tetragonoloba L.)作物序列进行田间试验。试验采用随机完全区(RCBD)的析因设计,有3个重复。第一个因素包括六个生产系统,即(PS1) -100%有机,(PS2) - 75%有机+ 25%创新实践(堆肥提取物,牛尿),(PS3) -综合(50%有机+ 50%无机),(PS4) -综合(75%有机+ 25%无机),(PS5) -100%无机营养源和(PS6) -国家推荐],包括茴香(Rabi季节)-集束豆(Kharif季节)种植序列,而第二个因素包括该时期的环境。结果:研究结果表明,集束豆(1501.87 kg ha-1)和茴香(1970.19 kg ha-1)以PS4 -一体化生产体系(75%有机+ 25%无机)的种子产量和产量属性最高。研究表明,在茴香和豆荚中,与PS5和PS1相比,生产体系PS4的蛋白质含量分别增加了17.29%和30.75%。在生产系统PS1下,土壤有机碳(~25.2%)和速效氮(~9%)、磷(~38.5%)和钾(~无显著性)的年增量分别比生产系统PS5增加了1%。茴香与集束豆籽粒产量、土壤有机碳、Shannon-Wiener指数分别呈显著正相关(P < 0.01)和显著正相关(R2 = 0.75、R2 = 0.69)。产量、质量、土壤有机碳和土壤可持续性综合评价表明,豆科作物轮作效果较好,为作物可持续集约化提供了模式。因此,在以茴香为主的农业生态系统中,在种植系统中添加豆科作物并实施综合营养管理(INM)可逐步改善土壤质量。
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引用次数: 0
Response of IWM Strategies on Growth, Nodulation, Productivity and Profitability in Fenugreek (Trigonella foenum-graecum L.) under Haryana Conditions IWM策略对哈里亚纳邦条件下胡芦巴生长、结瘤、生产力和盈利能力的响应
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-27 DOI: 10.18805/lr-5164
Isha Ahlawat, Todarmal ., Sumit Bhardwaj, K.K. Bhardwaj, Garima Dahiya, Abhishek ., Anjali Rana
Background: This research aims to analyze the effects of IWM strategies on growth, nodulation and nutrient uptake in Trigonella foenum-graecum L. under Haryana conditions. Methods: Field experiments were conducted for two successive years with Trigonella foenum-graecum L. sown at Agronomy Research farm (29°10'N, 75°46'E), CCS Haryana Agricultural University, Hisar, Haryana during 2018-19 and 2019-20 to investigate the impact of factors on growth and economics of Trigonella foenum-graecum L. Result: Among the integrated treatments, application of pendimethalin + imazethapyr (RM) at 1500 g ha-1 + one hoeing at 3-4 leaf stage recorded maximum nitrogen, phosphorus and potassium uptake by seed and haulm and was found to be at par with weed free check. PRE pendimethalin + imazethapyr (RM) at 1500 g ha-1 + one hoeing at 3-4 leaf stage provided highest net returns (Rs. 27,579 ha-1) and highest B: C (2.13) was observed in PRE pendimethalin + imazethapyr (RM) at 1500 g ha-1, respectively. This article aims to document the role of IWM and concludes that coupling pendimethalin and imazethapyr and supplementing them with one hand hoeing will provide effective weed management.
背景:本研究旨在分析在哈里亚纳邦条件下,IWM策略对葫芦巴生长、结瘤和营养吸收的影响。方法:2018-19年和2019-20年,连续两年在哈里亚纳邦希萨市哈里亚纳农业大学农学研究农场(29°10′n, 75°46′e)进行田间试验,研究影响葫芦巴生长和经济效益的因素。在综合处理中,喷施1500 g hm -1 + 3-4叶期1锄头的喷施双甲基萘啶+ imazethapyr (RM)对种子和茎秆氮、磷、钾的吸收量最大,与无草对照相当。1500 g ha-1的PRE - pendimealin + imazethapyr (RM) + 3-4叶期1锄头的净收益最高,为27,579 ha-1; 1500 g ha-1的PRE - pendimealin + imazethapyr (RM)的B: C最高,为2.13。本文的目的是记录IWM的作用,并得出结论,联用二甲甲基灵和咪唑噻韦并辅以单手锄地将提供有效的杂草管理。
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引用次数: 0
Management of Charcoal Rot (Macrophomina phaseolina) of Fenugreek in Rajasthan 拉贾斯坦邦葫芦巴炭腐病的防治
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-23 DOI: 10.18805/lr-5114
Santosh Kumari, A.K. Meena, Jasveer Singh, Rakesh Kumar, Vikash Kumar, Jasveer Singh
Background: Charcoal rot of fenugreek caused by Macrophomina phaseolina is destructive disease of all fenugreek growing areas of Rajasthan, The disease is wide occurrence in sandy soil of Rajasthan, where the climatic conditions are dry and temperature remains high. Under severe infestation it cause 35.00-42.56% losses in yield. In the present investigation, our main emphasis was to find out some new fungicides alone and combination with bio-agent for management of charcoal rot of fenugreek. Methods: Eight fungicides viz., captan 70%+ hexaconazole 5% WP, azoxystrobin 18.2% + difenaconazole 1.4% SC, chlorothalonil 75% WP, carbendazim 12% + mancozeb 63% WP, tebuconazole 50% + trifloxystrobin 25% WG, carboxin 37.5% + thiram 37.5% WP, copper oxychloride 50% WP and captan 70% WP were tested at different concentration of 100, 200, 300, 400 and 500 ppm against M. phaseolina using Poisoned Food Technique in vitro. The in vivo study was taken to evaluate the effect of one fungal and one bacterial bio-agent viz., T. harzianum, P. fluorescens was applied as seed treatment, soil dressing and soil application @ 5+5g/kg and @ 10 kg/ha respectively and fungicides viz., tebuconazole 50%+ trifloxystrobin 25% @ 1.5 g/kg, carbendazim 12% + mancozeb 63% @ 2 g/kg and azoxystrobin 18.2% + difenaconazole 11.4% 2 ml/kg seed were used in different treatments. The experiment was conducted at Experimental farm COA, SKRAU, Bikaner during Rabi 2020-21 on most popular cv. Rmt-305 in RBD design with the application of seed treatment, soil dressing and foliar spray of different fungicides with bioagents at different concentrations against Charcoal Rot disease and compared with an untreated control. Result: Among all the tested fungicides used in the present investigation, tebuconazole 50% + trifloxystrobin 25% WG found most effective in controlling the mycelium growth of pathogen. In field condition, it gave maximum disease control (85.72%) with highest grain yield (19.83 q/ha) when applied as seed treatment with tebuconazole 50% + trifloxystrobin 25% WG @ 1.5 g/kg seed + soil application of T. harzianum @ 10 kg/ha + SD with tebuconazole 50% + trifloxystrobin 25% WG. These treatments can provide an effective management of charcoal rot disease for fenugreek cultivators.
背景:葫芦巴炭腐病是拉贾斯坦邦所有葫芦巴种植区的破坏性病害,病害广泛发生在气候条件干燥、温度较高的沙质土壤中。严重侵染时造成产量损失35.00-42.56%。在本研究中,我们的重点是寻找一些新的杀菌剂单独或与生物制剂联合用于葫芦巴炭腐病的治理。方法:采用食品中毒技术,在100ppm、200ppm、300ppm、400ppm、500ppm的不同浓度条件下,分别测定了队长坦70%+六氟康唑5% WP、嘧菌酯18.2% +二苯康唑1.4% SC、百菌清75% WP、多菌灵12% +代森锰锌63% WP、戊唑唑50% +三氟菌酯25% WG、carboxin 37.5% + thiram 37.5% WP、氯化氧铜50% WP和队长坦70% WP等8种杀菌剂对稻瘟病菌的体外杀灭效果。采用体内试验的方法,评价了一种真菌和一种细菌生物制剂哈氏菌(T. harzianum, P. fluorescens)作为种子处理,土壤处理和土壤施用分别为@ 5+5g/kg和@ 10 kg/ha,不同处理的杀菌剂为:戊唑唑50%+三氟虫胺酯25% @ 1.5 g/kg,多菌灵12% +代森锰锌63% @ 2 g/kg和氮虫胺酯18.2% +地芬康唑11.4% 2 ml/kg。试验于2020-21年拉比期间在比卡内尔、SKRAU、COA试验农场进行。Rmt-305在RBD试验中采用种子处理、土壤处理和叶面喷施不同浓度杀菌剂防治炭腐病,并与未处理对照进行比较。结果:在本研究使用的杀菌剂中,50%戊康唑+ 25%三氯虫酯WG对病原菌菌丝生长的控制效果最好。在田间条件下,采用50%戊唑唑+ 25%三氯虫酯WG @ 1.5 g/kg种子+土施10 kg/ha土施50%戊唑唑+ 25%三氯虫酯WG SD处理,防治效果达85.72%,产量达19.83 q/ha。这些处理可以为胡芦巴栽培者提供有效的炭腐病管理。
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引用次数: 0
Influence of Morphological and Biochemical Parameters for Infection of Mungbean Yellow Mosaic Virus and Bemisia tabaci in Mungbean and Urdbean 绿豆黄花叶病毒和烟粉虱侵染绿豆和乌豆的形态学和生化参数的影响
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-23 DOI: 10.18805/lr-5144
L.K. Vidyashree, Gururaj Sunkad, Venkatesh ., G. Sugeetha, N.S. Pankaja
Background: Mungbean and Urdbean are the important pulses in India which are regarded as prospective protein source for human daily diet and rich source of protein and carbohydrate. Mungbean Yellow Mosaic Virus is one of the destructive diseases to pulses and transmitted by whitefly. Among the pulses, mungbean and urdbean are severely affected by MYMV in India. Present investigation was aimed to determine the congenial and mitigating factors of mungbean and urdbean crops with reference to infection of MYMV and its vector. Methods: The morphological and biochemical analysis was carried out by factorial RCBD consisting of two factors viz., factor 1: Crops (V) (Mung bean and Urd bean) and factor 2: observation at 15 days interval up to 90 DAS (S) for recording different morphological and biochemical parameters. Result: Morphological parameters of urdbean are able to mitigate the vector incidence and indirectly reduced the MYMV infection in urdbean than mungbean. Further, the biochemical parameters levels were more in urdbean than mungbean and their levels increased with the increased MYMV incidence. Hence, it is concluded that the morphological characters are found responsible for vector infestation where as biochemical levels are important for the infection of MYMV in both the crops.
背景:绿豆和乌豆是印度重要的豆类,被视为人类日常饮食中潜在的蛋白质来源和丰富的蛋白质和碳水化合物来源。绿豆黄花叶病毒是一种通过粉虱传播的对豆类具有破坏性的病害。在豆类中,绿豆和黑豆在印度受到MYMV的严重影响。本研究旨在结合MYMV及其病媒的侵染情况,确定绿豆和豇豆作物的适宜和缓解因素。方法:采用因子RCBD进行形态学和生化分析,因子1:作物(V)(绿豆和乌豆);因子2:每隔15 d观察90 DAS (S),记录不同的形态学和生化参数。结果:与绿豆相比,蚕豆的形态参数能够减轻病媒的发病率,间接降低MYMV在蚕豆中的感染。此外,赤豆的生化指标水平高于绿豆,且随着MYMV发病率的增加而升高。因此,我们得出结论,形态特征是媒介侵染的原因,而生化水平对两种作物的MYMV侵染都很重要。
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引用次数: 0
Studies on the Efficacy and Management of Seed Borne Diseases and its Economics in Chickpea (Cicer arietinum L.) 鹰嘴豆(Cicer arietinum L.)种传病害防治效果及经济学研究
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-23 DOI: 10.18805/lr-5077
Gayatri Khandappa Ravaloji, Ashok S. Sajjan, Basamma B. Kumbar
Background: Seed borne diseases are regarded as major constraints in chickpea production. Seed diseases are caused by biotic factor like fungi and abiotic especially by drought, resulted in decrease in seed yield and productivity. Hence studies on the efficacy and management of seed borne diseases and its economics of seed production in chickpea was attempted. Methods: The field experiment was carried out in Regional Agricultural Research Station, College of Agriculture, Vijayapur during rabi 2021-2022. The field experiment consisted eight treatments of fungicides and bioagents and combi- product of fungicide and bioagents viz., T1: Carboxin 37% + thiram 37% DS @ 2 g/kg of seeds, T2: Carbendazim 25% + mancozeb 50% WS @ 3.5 g/kg of seed, T3: Thiophanatemethyl 45% + pyraclostrobin 5% FS @ 4 ml /kg of seeds, T4: Seed treatment with Pseudomonas fluorescens @ 10/kg of seed, T5: Seed treatment with Trichoderma harzianum @ 10/kg of seed, T6: Seed treatment with Bacillus thuringenesis @ 10/kg of seed, T7: Seed treatment with Trichoderma harzianum @ 10/kg of seed + carbendazim 25% + mancozeb 50% WS @ 3.5 g/kg of seed T8: Control (without seed treatment) were used to study the efficacy of treatments on growth yield and economics and per cent incidence against R. bataticola in field condition. Result: Among the seed treatments carbendazim 25% + mancozeb 50% WS @ 3.5 g/kg of seed recorded significantly higher growth, seed yield, seed quality parameters and reduced per cent disease incidence and higher economics followed bythiophanatemethy 45% + pyraclostrobin 5% FS @ 4 ml/kg of seeds, as compared to control.
背景:种子传播疾病被认为是鹰嘴豆生产的主要制约因素。种子病害是由真菌等生物因子和非生物因子引起的,尤其是干旱引起的,导致种子产量和生产力下降。因此,对鹰嘴豆种子传播疾病的防治效果、管理及种子生产的经济性进行了研究。方法:2021-2022年rabi期间在维贾雅普尔农业学院区域农业研究站进行田间试验。田间试验包括8个杀菌剂和生物制剂及杀菌剂和生物制剂组合处理,即T1: Carboxin 37% + thiram 37% DS @ 2 g/kg种子,T2:多菌灵25% +代森锰锌50% WS @ 3.5 g/kg种子,T3:硫胺乙酯45% +吡唑菌酯5% FS @ 4 ml /kg种子,T4:荧光假单胞菌种子处理@ 10/kg种子,T5:哈茨木霉种子处理@ 10/kg种子,T6:采用苏云根芽孢杆菌@ 10/kg种子处理,T7:哈茨木霉@ 10/kg种子+多菌灵25% +代森锰锌50% WS @ 3.5 g/kg种子处理。T8:对照(不进行种子处理),研究田间条件下不同处理对生长产量、经济效益和巴塔提科拉病发病率的影响。结果:与对照相比,多菌灵25% +代森锌50% WS @ 3.5 g/kg的种子处理显著提高了种子的生长、产量和质量参数,降低了百分之百的发病率,经济效益更高,其次是磺胺硫醚45% +吡唑菌酯5% FS @ 4 ml/kg的种子处理。
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引用次数: 0
Exploration of the Potential of Bacillus spp. as an Antagonist and PGPR against Stem and Pod Rot of Groundnut. 芽孢杆菌作为花生茎腐病拮抗剂和PGPR潜力的探索。
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-20 DOI: 10.18805/lr-5100
Gururaj Sunkad, Khadarbi ., Meghana S. Patil, Ranjana Joshi
Background: Stem and pod rot is an economically important soil borne disease of groundnut caused by Sclerotium rolfsii Sacc. resulting in significant yield losses across the globe. Bacillus spp. is rod-shaped and gram-positive bacterium which is predominantly found in the soil. Hence, considering the importance of the disease, the present investigation was carried out to evaluate the indigenous Bacillus spp. for plant growth promotion and to inhibit the pathogen in groundnut. Methods: Totally nineteen Bacillus spp. isolates from healthy groundnut rhizosphere and their antagonistic potential was recorded. The molecular characterization of five efficient Bacillus spp. was carried out using 16S rDNA. Later, morphological and biochemical characterization and plant growth promoting traits were recorded. Result: All nineteen isolates of Bacillus spp. showed antagonistic potential against S. rolfsii. Five Bacillus spp. isolates which showed higher inhibition of the pathogen were identified as B. subtilis, B. pacific, B. cereus, two isolates belonging to B. tropicus through molecular technique. The morphological, biochemical characters and growth promoting traits were recorded.
背景:花生茎、荚果腐病是由罗氏菌核菌引起的一种重要的土传病害。导致全球范围内的重大产量损失。芽孢杆菌是一种杆状革兰氏阳性细菌,主要存在于土壤中。因此,考虑到该病害的重要性,本研究旨在评价本地芽孢杆菌对花生植株生长的促进作用和对病原菌的抑制作用。方法:对健康花生根际分离的19株芽孢杆菌进行拮抗活性测定。利用16S rDNA对5株高效芽孢杆菌进行了分子鉴定。随后,对其形态生化特性和促进植株生长的性状进行记录。结果:19株芽孢杆菌均对罗氏葡萄球菌有拮抗作用。通过分子技术鉴定出对病原菌有较强抑制作用的5株芽孢杆菌分别为枯草芽孢杆菌、太平洋芽孢杆菌、蜡样芽孢杆菌,2株属热带芽孢杆菌。记录了其形态、生化特性和促生长性状。
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引用次数: 0
Integrated use of Consortia-based Microbial Inoculants and Nutrient Complex Stimulates the Rhizosphere Microbiome and Soybean Productivity 综合利用菌丝体微生物接种剂和营养复合物刺激根际微生物群和大豆生产力
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-20 DOI: 10.18805/lrf-762
D. Miljakoviæ, J. Marinkoviæ, V. Ðorðeviæ, M. Vasiljeviæ, B. Tintor, S. Jaæimoviæ, Ž. Ristiæ
Background: The effectiveness of soybean inoculants in field conditions may be improved through the joint application of rhizobia and plant growth-promoting bacteria (PGPB). Plant nutrients may also contribute to the higher efficacy of microbial inoculants as well as the growth and development of plants. Methods: A field experiment was performed to evaluate the responses of the rhizomicrobiome and soybean to seed treatments with multiple bacterial strains (Bradyrhizobium japonicum, Bacillus subtilis, Bacillus megaterium and Azotobacter chroococcum), applied individually or in consortia, with and without nutrient complex (S, Mg, Mn, Fe, Zn, Cu, B and Mo). Result: Seed treatments with microbial consortia of Br. japonicum, B. subtilis/B. megaterium, A. chroococcum strains and nutrients had the highest effect on the abundance of total bacteria, nitrogen-fixing bacteria, actinomycetes and activity of dehidrogenase in the soybean rhizosphere. The highest effect on plant height, plant weight, pod number, pod weight, seed number and seed weight was obtained from treatment with Br. japonicum strains with nutrients, followed by co-inoculation with B. megaterium and A. chroococcum. In comparison with the control and Bradyrhizobium single inoculation, a statistically significant increase in the seed yield was recorded in treatment with Br. japonicum, B. megaterium, A. chroococcum and nutrients, reflecting the highest increase in protein and oil yield. The interactive effects of microbial consortia and nutrients could be used as promising seed technology for sustainable soybean cropping.
背景:通过根瘤菌和植物生长促进菌的联合施用,可以提高大豆接种剂在田间条件下的效果。植物营养素也可能有助于微生物接种剂的更高功效以及植物的生长和发育。方法:采用田间试验方法,研究不同菌种(日本慢生根瘤菌、枯草芽孢杆菌、巨芽孢杆菌和固氮杆菌)单独或联合施用,外加和不加营养物(S、Mg、Mn、Fe、Zn、Cu、B和Mo)对大豆根际微生物组和大豆的影响。结果:用Br菌群处理种子。枯草芽孢杆菌;对大豆根际总细菌、固氮细菌、放线菌丰度和脱氢酶活性影响最大的是巨芽孢杆菌、绿芽孢杆菌和营养物质。对株高、株重、荚果数、荚果重、种子数和种子重影响最大的是溴处理。然后与巨芽孢杆菌和绿芽孢杆菌共接种。与对照和单次接种缓生根瘤菌相比,Br处理的种子产量有统计学意义的显著提高。其中,蛋白质和油脂产量的增加幅度最大。微生物群落与养分的互作效应可作为大豆可持续种植的一种有前景的种子技术。
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引用次数: 0
Effect of Different Biochemical Parameters and Antioxidant Enzymes Activities on Drought Indices in Chickpea (Cicer arietinum L.) 不同生化参数及抗氧化酶活性对鹰嘴豆干旱指标的影响
4区 生物学 Q3 Agricultural and Biological Sciences Pub Date : 2023-10-19 DOI: 10.18805/lr-5204
Rajitha Sistu, Sushma Tiwari, M.K. Tripathi, Sangeeta Singh, Neha Gupta, Niraj Tripathi, Ruchi Asati, Rakesh Kumar Yadav
Background: Drought is the foremost environmental restraint that effects the growth and yield of chickpea. The mechanism of response to drought differs with genotype and growth stages of individual crop species. The activation of antioxidant enzymes is an alternate defensive system against oxidative stress that ultimately decide development of putative drought tolerant mechanism. Methods: Eighty-three chickpea genotypes were grown under normal and drought stress conditions and leaf samples were used to estimate different biochemical parameters including total sugar, lipid peroxidation (MDA), chlorophyll, proline and protein along with estimation of activities of different antioxidant enzymes viz., catalase (CAT) (EC 1.11.1.6), ascorbate peroxidase (APX) (EC 1.11.1.11), superoxide dismutase (SOD) and peroxidase (POX). Result: Positively significant correlation was found among proline under control with proline underwent stress (r=0.441), MDA under stress with proline under control (r=0.365) and MDA under control (r=0.336) at 1% level of significance. Positively significant correlation was also investigated between SOD under stressed condition with SOD under control (r=0.665), POX (0.449) and APX under stress (0.423), CAT under control (0.471) and CAT under stress condition (0.374) at 1% probability level. Heatmaps along with dendrograms represented expression levels of different antioxidant enzymes activities that showed variations among different genotypes. In conclusion. total sugar, proline and Malondialdehyde, have been increased under drought stressed condition whilst total chlorophyll and protein were decreased. While antioxidant enzymes viz., POX, APX, CAT and SOD levels increased under drought stressed conditions.
背景:干旱是影响鹰嘴豆生长和产量的首要环境制约因素。不同作物品种对干旱的响应机制因基因型和生长阶段而异。抗氧化酶的激活是抗氧化胁迫的一种替代防御系统,最终决定了推定的抗旱机制的发展。方法:在正常和干旱胁迫条件下培养83种鹰嘴豆基因型,利用叶片样品测定其总糖、脂质过氧化(MDA)、叶绿素、脯氨酸和蛋白质等生化指标,并测定其过氧化氢酶(CAT) (EC 1.11.1.6)、抗坏血酸过氧化物酶(APX) (EC 1.11.1.11)、超氧化物歧化酶(SOD)和过氧化物酶(POX)的活性。结果:受控制的脯氨酸与受胁迫的脯氨酸呈显著正相关(r=0.441),受胁迫的MDA与受控制的脯氨酸呈显著正相关(r=0.365),受控制的MDA呈显著正相关(r=0.336),在1%的显著水平上。胁迫条件下SOD与对照SOD (r=0.665)、POX (r= 0.449)与APX (r= 0.423)、CAT (r= 0.471)与CAT (r= 0.374)呈1%概率正相关。热图和树状图代表了不同抗氧化酶活性的表达水平,显示了不同基因型之间的差异。在结论。总糖、脯氨酸和丙二醛含量在干旱胁迫下增加,总叶绿素和总蛋白质含量降低。而抗氧化酶(即POX、APX、CAT和SOD)水平在干旱胁迫下升高。
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引用次数: 0
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Legume Research
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