Antibacterial efficacy and osteogenic potential of mineral trioxide aggregate-based retrograde filling material incorporated with silver nanoparticle and calcium fluoride

IF 3.4 3区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE Journal of Dental Sciences Pub Date : 2024-07-01 DOI:10.1016/j.jds.2023.10.001
Min-Yong Lee , Hi-Won Yoon , Kwang-Mahn Kim , Jae-Sung Kwon
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Abstract

Background/purpose

The retrograde filling material, particularly mineral trioxide aggregate (MTA) employed in apicoectomy, should possess high antibacterial efficacy and osteogenic potential. We evaluated the antibacterial efficacy, biocompatibility, and osteogenic potential following the addition of silver nanoparticles (AgNPs) and calcium fluoride (CaF2) in retrograde filling material of MTA.

Materials and methods

MTA was mixed with four different solvents. Group 1 (G1): distilled water, Group 2 (G2): 50 ppm AgNPs, Group 3 (G3): 1 wt% CaF2, and Group 4 (G4): 50 ppm AgNPs and 1 wt% CaF2. The pH variation of each group was monitored, while the surface roughness was measured. The antibacterial efficacy against Enterococcus faecalis (E. faecalis) and the viability of murine pre-osteoblast (MC3T3) were evaluated for each group using colorimetric assays. The gene expression levels of osteogenic potential marker (OCN, ALPL, and RUNX2) in MC3T3 cells for each group were quantified using real-time-qPCR. Statistical analysis was performed at α = 0.05 level of significance.

Results

When comparing the levels of antibacterial efficacy, the order of effectiveness was G4>G2>G3>G1 (P < 0.05). In the cell viability test, owing to MTA-eluted growth medium having a positive effect on MC3T3 cell proliferation, G1–4 exhibited a statistically increased cell viability compared to the control (P < 0.05). However, G2–4 did not result in a statistically significant difference when compared to G1 (P < 0.05). Moreover, G4 exhibited the highest gene expression among the four groups (P < 0.05).

Conclusion

The addition of AgNPs and CaF2 to MTA could be a promising option for use as a new retrograde filling material.

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加入纳米银粒子和氟化钙的三氧化二矿骨料逆行填充材料的抗菌功效和成骨潜力
背景/目的根尖切除术中使用的逆行充填材料,尤其是三氧化二铝矿物骨料(MTA),应具有较高的抗菌功效和成骨潜力。我们评估了在 MTA 逆行充填材料中添加纳米银(AgNPs)和氟化钙(CaF2)后的抗菌效果、生物相容性和成骨潜力。第一组(G1):蒸馏水;第二组(G2):50 ppm AgNPs;第三组(G3):50 ppm AgNPs:50 ppm AgNPs;第 3 组(G3):1 wt% CaF2;第 4 组(G3):1 wt% AgNPs:1 wt% CaF2,以及第 4 组(G4):50 ppm AgNPs 和 1 wt% CaF2。监测了各组的 pH 值变化,同时测量了表面粗糙度。使用比色法评估了各组对粪肠球菌(E. faecalis)的抗菌效果和小鼠前成骨细胞(MC3T3)的活力。各组 MC3T3 细胞成骨潜能标志物(OCN、ALPL 和 RUNX2)的基因表达水平采用实时 qPCR 法进行量化。结果比较各组的抗菌效果,效果依次为 G4>G2>G3>G1 (P < 0.05)。在细胞存活率测试中,由于 MTA 稀释生长培养基对 MC3T3 细胞增殖有积极影响,因此与对照组相比,G1-4 的细胞存活率在统计学上有所提高(P <0.05)。然而,与 G1 相比,G2-4 在统计学上没有显著差异(P < 0.05)。结论在 MTA 中添加 AgNPs 和 CaF2 可作为一种新型逆行充填材料,是一种很有前景的选择。
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来源期刊
Journal of Dental Sciences
Journal of Dental Sciences 医学-牙科与口腔外科
CiteScore
5.10
自引率
14.30%
发文量
348
审稿时长
6 days
期刊介绍: he Journal of Dental Sciences (JDS), published quarterly, is the official and open access publication of the Association for Dental Sciences of the Republic of China (ADS-ROC). The precedent journal of the JDS is the Chinese Dental Journal (CDJ) which had already been covered by MEDLINE in 1988. As the CDJ continued to prove its importance in the region, the ADS-ROC decided to move to the international community by publishing an English journal. Hence, the birth of the JDS in 2006. The JDS is indexed in the SCI Expanded since 2008. It is also indexed in Scopus, and EMCare, ScienceDirect, SIIC Data Bases. The topics covered by the JDS include all fields of basic and clinical dentistry. Some manuscripts focusing on the study of certain endemic diseases such as dental caries and periodontal diseases in particular regions of any country as well as oral pre-cancers, oral cancers, and oral submucous fibrosis related to betel nut chewing habit are also considered for publication. Besides, the JDS also publishes articles about the efficacy of a new treatment modality on oral verrucous hyperplasia or early oral squamous cell carcinoma.
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