Journal of Dental Sciences最新文献

Prevention of medication-related osteonecrosis of the jaw after tooth extraction by local administration of antibiotics and atelocollagen sponge: A preliminary study 通过局部使用抗生素和阿特劳胶原海绵预防拔牙后与药物相关的颌骨坏死：初步研究

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2024.04.006

Natsumi Nakamura , Sakiko Soutome , Akira Imakiire , Satoshi Rokutanda , Seigo Ohba , Shunsuke Sawada , Yuka Kojima , Yuki Sakamoto , Yoshiko Yamamura , Madoka Funahara , Mitsunobu Otsuru , Masahiro Umeda

Background/purpose

Medication-related osteonecrosis of the jaw (MRONJ) often develops after extraction of a tooth with a local infection. Therefore, it is necessary to develop extraction methods that can prevent MRONJ. Before examining whether antibiotics and atelocollagen administered in the extraction socket can prevent the development of MRONJ after tooth extraction, this study was conducted to determine the appropriate antibiotics concentration and to conduct a clinical study with a small number of cases as a preliminary study.

Materials and methods

First, a mixture of minocycline and atelocollagen at different concentrations was implanted into the bone cavity formed in the rabbit head, and the local minocycline concentration was measured after 24 and 48 h. Next, the incidences of MRONJ after tooth extraction in patients receiving high-dose antiresorptive agents were compared between the atelocollagen and atelocollagen/minocycline mixture groups. A group that did not undergo transplantation was also compared as a historical control.

Results

In animal studies, a mixture of 10 mg/ml minocycline injected into collagen and implanted into the bone cavity showed sufficiently high antimicrobial concentrations, even after 48 h. Post-extraction MRONJ occurred in 3 of the 13 control groups (23.1%), 3 of the 13 atelocollagen groups (23.1%), and 1 of the 13 atelocollagen/minocycline groups (7.7%).

Conclusion

Atelocollagen functions as a carrier for retaining antibiotics for a certain period. Although this was a study with a limited number of cases, it suggested that the local administration of atelocollagen/minocycline in the extraction socket may reduce the risk of MRONJ following tooth extraction.

背景/目的药物相关性颌骨骨坏死（MRONJ）通常发生在局部感染的拔牙后。因此，有必要开发能够预防MRONJ的提取方法。在研究拔牙槽内使用抗生素和间胶原蛋白是否可以预防拔牙后MRONJ的发生之前，本研究确定合适的抗生素浓度，并进行少量病例的临床研究作为初步研究。材料与方法首先将不同浓度的米诺环素与间胶原的混合物植入兔头部形成的骨腔内，在24和48 h后测量局部米诺环素浓度。然后比较大剂量抗吸收药物组和间胶原/米诺环素混合物组拔牙后MRONJ的发生率。未接受移植的一组也作为历史对照进行比较。结果在动物实验中，将10 mg/ml米诺环素注入胶原蛋白并植入骨腔，48 h后仍显示出足够高的抗菌浓度。提取后MRONJ在13个对照组中有3个（23.1%），在13个胶原蛋白组中有3个（23.1%），在13个胶原蛋白/米诺环素组中有1个（7.7%）发生。结论胶原蛋白可作为一种载体，在一定时期内保留抗生素。虽然这是一项病例数量有限的研究，但它表明，在拔牙槽内局部使用阿特罗胶原/米诺环素可能会降低拔牙后MRONJ的风险。

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引用次数: 0

Correlation between dental amalgam fillings and urinary mercury levels in the Jordanian population: A comparative population-based study 牙科汞合金填充物与约旦人群尿汞水平的相关性：一项基于人群的比较研究

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.05.022

Ibrahim Al-Odat , Saad Al-Fawaeir , Naser Odat

Background/purpose

The health risks of mercury-containing dental amalgam fillings remain controversial. Urinary mercury levels are used as a tool to assess mercury exposure toxicity. Therefore, this study aimed to examine the effects of dental amalgam fillings on urinary mercury levels in the Jordanian population.

Materials and methods

This study recruited an experimental group of 108 participants with dental amalgam fillings and control group of 109 participants without dental amalgam fillings. Urinary mercury levels were measured and compared between the two groups.

Results

The results showed a statistically significant increase in urinary mercury concentration in participants with dental amalgam fillings compared to participants with no dental amalgam fillings (6.42 ± 0.37 μg/L vs 1.55 ± 0.05 μg/L, P < 0.05). It also showed that this increase in urinary mercury levels among Jordanians with dental amalgam fillings was statistically significant when individuals had more than 6 dental amalgam fillings (9.36 ± 1.68 μg/L) compared to those with fewer than 6 (4.73 ± 0.34 μg/L, P < 0.05). Increased urinary mercury levels among Jordanians were also correlated with having dental amalgam fillings for more than 10 years.

Conclusion

Increased urinary mercury levels in the Jordanian population are correlated with both the number of amalgam fillings and the duration of having these fillings. However, this increase in urinary mercury levels observed in this study is below the threshold that would pose significant health risks.

背景/目的含汞牙科汞合金填充物的健康风险仍然存在争议。尿汞水平被用作评估汞接触毒性的工具。因此，本研究旨在研究牙科汞合金填充物对约旦人口尿汞水平的影响。材料与方法采用牙科汞合金充填物的试验组108例，未采用牙科汞合金充填物的对照组109例。测量并比较两组患者的尿汞水平。结果口腔汞合金充填组尿汞浓度明显高于未充填组（6.42±0.37 μg/L vs 1.55±0.05 μg/L, P < 0.05）。研究还表明，在使用牙科汞合金填充物的约旦人中，使用超过6个牙科汞合金填充物的人尿汞水平（9.36±1.68 μg/L）高于使用少于6个牙科汞合金填充物的人（4.73±0.34 μg/L, P < 0.05），差异有统计学意义。约旦人尿液中汞含量的增加也与使用汞合金填充物超过10年有关。结论约旦人群尿汞水平升高与汞合金充填次数和充填时间有关。然而，本研究中观察到的尿汞水平的增加低于会造成重大健康风险的阈值。

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引用次数: 0

Zygomatic fracture characteristics and their association with brain injury: Evidences from a 10-year retrospective study in northern Taiwan 颧骨骨折特征及其与脑损伤的关系：来自台湾北部10年回顾性研究的证据

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.002

Wei-Che Tsai , Chen-Yin Yong , Sam Li-Sheng Chen , Yoichi Ohiro , Chih-Yuan Fang

Background/purpose

Few studies have systematically examined the relationship between zygomatic fracture patterns or patient demographics and head injury. This study investigates the epidemiology of zygomatic fractures, their association with brain injury, and the influence of patient age, focusing on fracture classification and injury mechanisms.

Materials and methods

A retrospective analysis of 272 patients with zygomatic fractures treated between 2012 and 2021 at a medical center in northern Taiwan was conducted. Fractures were classified using the Zingg system. Clinical data including age, injury etiology, concomitant injuries, and brain injury were analyzed. Statistical methods included chi-square tests, one-way ANOVA, and multiple logistic regression.

Results

Motorcycle accidents were the most common cause of zygomatic fractures (43.4 %), followed by falls (27.9 %). The highest incidence occurred in patients aged 61–70 years. Older patients had significantly lower surgical intervention rates (P < 0.05). Type B fractures were most common (51.8 %), and bilateral fractures were significantly associated with higher rates of brain injury (58.3 % vs. 26.6 %, P < 0.05). Among patients with fall-related fractures, those with brain injury were significantly older than those without (P = 0.008). Even low-energy trauma, such as ground-level falls, led to brain injury in elderly patients. In younger individuals, brain injury was associated with bilateral fractures and concomitant injuries, whereas in older patients, age itself was a major risk factor for brain injury.

Conclusion

Falls are increasingly associated with zygomatic fractures in older adults, and even minor trauma can result in brain injury. Bilateral fractures and high-energy mechanisms remain key predictors of brain injury in younger patients.

背景/目的很少有研究系统地探讨颧骨骨折类型或患者人口统计学与头部损伤之间的关系。本研究探讨了颧骨骨折的流行病学、与脑损伤的关系以及患者年龄的影响，重点研究了骨折的分类和损伤机制。材料与方法回顾性分析台湾北部某医疗中心2012 - 2021年间收治的272例颧骨骨折患者。采用Zingg系统对裂缝进行分类。临床资料包括年龄、损伤病因、伴发损伤和脑损伤。统计方法包括卡方检验、单因素方差分析和多元逻辑回归。结果摩托车事故是导致颧骨骨折最常见的原因（43.4%），其次是跌倒（27.9%）。发病率最高的患者年龄为61-70岁。老年患者手术干预率明显低于老年患者（P < 0.05）。B型骨折最为常见（51.8%），双侧骨折与颅脑损伤发生率显著相关（58.3%比26.6%,P < 0.05）。在跌倒相关骨折患者中，有脑损伤的患者年龄明显大于无脑损伤的患者（P = 0.008）。即使是低能量的创伤，如地面坠落，也会导致老年患者脑损伤。在年轻人中，脑损伤与双侧骨折和伴随损伤有关，而在老年患者中，年龄本身是脑损伤的主要危险因素。结论老年人颧骨骨折与跌倒的关系日益密切，即使是轻微的外伤也可能导致脑损伤。双侧骨折和高能机制仍然是年轻患者脑损伤的关键预测因素。

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引用次数: 0

Suppression of distal-less homeobox 3 increased alveolar bone mass in mouse tooth socket by targeting thrombospondin 1 抑制无远端同源盒3通过靶向血栓反应蛋白1增加小鼠牙窝牙槽骨质量

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.07.008

Chang Diao , Fang Li , Kai Sun , Miao Yu , Haochen Liu , Hailan Feng , Yang Liu , Dong Han

Background/purpose

Alveolar ridge resorption after tooth extraction significantly impacts dental implantation and prosthodontic treatment outcomes. Sustaining alveolar bone mass remains a critical clinical challenge and a major focus of research. Notably, patients with tricho-dento-osseous (TDO) syndrome, characterized by distal-less homeobox 3(DLX3) loss-of function mutations, exhibit increased jaw bone density and demonstrate long-term preservation of alveolar bone mass post-tooth extraction. This study aimed to investigate the effects of DLX3 knockdown on bone remodeling following tooth extraction and to elucidate the underlying molecular mechanisms.

Materials and methods

we established a murine maxillary incisor extraction model to investigate alveolar bone healing. An empty adenoviral vector or an adenoviral vector designed to suppress Dlx3 gene expression was delivered into the alveolar sockets of wild-type C57BL/6 mice, respectively. Histological staining, micro-computed tomography (micro-CT), immunohistochemistry, immunofluorescence, and RNA sequencing were employed to evaluate the outcomes.

Results

Histological analysis revealed accelerated bone formation and increased alveolar bone mass in the Dlx3-knockdown group. This phenotype was attributed to the activation of osteoblasts, which promoted new bone formation, and the inhibition of osteoclasts, which reduced bone resorption, without compromising normal bone structure of newly formed bone. RNA sequencing identified thrombospondin 1(Thbs1) as a key downstream gene. Downregulation of Thbs1 following Dlx3-knockdown enhanced angiogenesis and osteogenesis. Conversely, the addition of recombinant THBS1 protein after Dlx3 inhibition partially reversed the enhanced osteogenic phenotype.

Conclusion

Suppression of Dlx3 increased alveolar bone mass by down-regulation of Thbs1. Our study provides a theoretical foundation for developing novel strategies to preserve alveolar bone.

背景/目的拔牙后牙槽嵴吸收对种植和修复效果有显著影响。维持牙槽骨质量仍然是一个关键的临床挑战和研究的主要焦点。值得注意的是，以无远端同源盒3（DLX3）功能缺失突变为特征的毛牙-牙-骨（TDO）综合征患者表现出颌骨骨密度增加，并在拔牙后长期保存牙槽骨量。本研究旨在探讨DLX3基因下调对拔牙后骨重塑的影响，并阐明其潜在的分子机制。材料与方法建立小鼠上颌切牙拔牙模型，观察牙槽骨愈合情况。将空腺病毒载体或设计抑制Dlx3基因表达的腺病毒载体分别送入野生型C57BL/6小鼠的牙槽窝。采用组织学染色、显微计算机断层扫描（micro-CT）、免疫组织化学、免疫荧光和RNA测序来评估结果。结果组织学分析显示dlx3基因敲低组骨形成加快，牙槽骨质量增加。这种表型归因于成骨细胞的激活，促进新骨的形成，以及破骨细胞的抑制，减少骨吸收，而不影响新形成骨的正常骨结构。RNA测序发现血栓反应蛋白1（Thbs1）是一个关键的下游基因。dlx3敲除后Thbs1的下调增强了血管生成和骨生成。相反，在Dlx3抑制后加入重组THBS1蛋白部分逆转了增强的成骨表型。结论Dlx3抑制通过下调Thbs1增加牙槽骨骨量。我们的研究为开发新的牙槽骨保护策略提供了理论基础。

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引用次数: 0

Novel MMP20 (matrix metalloproteinase 20) mutations causing hypoplastic-hypomaturation amelogenesis imperfecta 新的MMP20（基质金属蛋白酶20）突变导致发育不全-不饱和变性不完全性

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.039

Shih-Kai Wang , Hong Zhang , Hua-Chieh Lin , Yin-Lin Wang , J. Timothy Wright , John D. Bartlett , James P. Simmer , Jan C.-C. Hu

Background/purpose

Matrix metalloproteinase 20 (MMP20) is a proteinase essential for dental enamel formation. Mutations in human MMP20 cause autosomal recessive amelogenesis imperfecta (AI), characterized by thin and soft enamel. This study aimed to unravel the genetic causes for five families with hypoplastic-hypomaturation AI.

Materials and methods

Whole-exome analyses and Sanger sequencing were performed to identify and confirm disease-causing mutations. To evaluate the pathogenicity of identified MMP20 missense variants, immunoblotting and gelatin zymography were conducted on proteins overexpressed in HEK293T cells.

Results

All affected individuals from the five families exhibited similar dental phenotypes, including chalky-white to yellow-brown discolorations and evident dental attrition. The defective enamel was both thin and hypomineralized. Six pathogenic MMP20 variants were identified: c.289A>T (p.Lys97∗), c.547G>A (p.Asp183Asn), c.686G>A (p.Gly229Asp), c.102G>A (p.Trp34∗), c.359dup (p.Asn120Lysfs∗9), and c.954-2A>T. Among them, the first three have not been previously reported. The two missense mutations altered evolutionarily conserved amino acid residues within the catalytic domain of MMP20. Compared with the wild type, secretion of both mutant MMP20 proteins was significantly impeded, and neither displayed proteolytic activity on gelatin zymography, indicating a loss of enzymatic function.

Conclusion

This study expands the genotypic spectrum of MMP20-associated AI and highlights two critical residues within the MMP20 catalytic domain that are essential for its secretion and enzymatic activity.

背景/目的基质金属蛋白酶20 （matrix metalloproteinase 20, MMP20）是牙釉质形成所必需的一种蛋白酶。人类MMP20基因突变导致常染色体隐性无釉发育不全症（AI），其特征是釉质薄而软。本研究旨在揭示发育不良-低饱和度AI的5个家族的遗传原因。材料和方法采用全外显子组分析和Sanger测序来鉴定和确认致病突变。为了评估鉴定出的MMP20错义变异的致病性，我们对HEK293T细胞中过表达的蛋白进行了免疫印迹和明胶酶谱分析。结果5个家族的患者均表现出相似的牙齿表型，包括白垩白至黄褐色变色和明显的牙齿磨损。有缺陷的牙釉质既薄又低矿化。鉴定出6个致病MMP20变异：c.289A>T （p.Lys97∗）、c.547G>A （p.Asp183Asn）、c.686G>A （p.Gly229Asp）、c.102G>A （p.Trp34∗）、c.359dup （p.Asn120Lysfs∗9）和c.954-2A>；T。其中，前三种以前没有报道过。这两个错义突变改变了MMP20催化结构域中进化上保守的氨基酸残基。与野生型相比，两种突变型MMP20蛋白的分泌都明显受阻，明胶酶谱图显示两种突变型MMP20蛋白都没有水解活性，表明酶功能丧失。本研究扩大了MMP20相关AI的基因型谱，并突出了MMP20催化结构域中对其分泌和酶活性至关重要的两个关键残基。

{"title":"Novel MMP20 (matrix metalloproteinase 20) mutations causing hypoplastic-hypomaturation amelogenesis imperfecta","authors":"Shih-Kai Wang , Hong Zhang , Hua-Chieh Lin , Yin-Lin Wang , J. Timothy Wright , John D. Bartlett , James P. Simmer , Jan C.-C. Hu","doi":"10.1016/j.jds.2025.08.039","DOIUrl":"10.1016/j.jds.2025.08.039","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Matrix metalloproteinase 20 (MMP20) is a proteinase essential for dental enamel formation. Mutations in human <em>MMP20</em> cause autosomal recessive amelogenesis imperfecta (AI), characterized by thin and soft enamel. This study aimed to unravel the genetic causes for five families with hypoplastic-hypomaturation AI.</div></div><div><h3>Materials and methods</h3><div>Whole-exome analyses and Sanger sequencing were performed to identify and confirm disease-causing mutations. To evaluate the pathogenicity of identified <em>MMP20</em> missense variants, immunoblotting and gelatin zymography were conducted on proteins overexpressed in HEK293T cells.</div></div><div><h3>Results</h3><div>All affected individuals from the five families exhibited similar dental phenotypes, including chalky-white to yellow-brown discolorations and evident dental attrition. The defective enamel was both thin and hypomineralized. Six pathogenic <em>MMP20</em> variants were identified: c.289A>T (p.Lys97∗), c.547G>A (p.Asp183Asn), c.686G>A (p.Gly229Asp), c.102G>A (p.Trp34∗), c.359dup (p.Asn120Lysfs∗9), and c.954-2A>T. Among them, the first three have not been previously reported. The two missense mutations altered evolutionarily conserved amino acid residues within the catalytic domain of MMP20. Compared with the wild type, secretion of both mutant MMP20 proteins was significantly impeded, and neither displayed proteolytic activity on gelatin zymography, indicating a loss of enzymatic function.</div></div><div><h3>Conclusion</h3><div>This study expands the genotypic spectrum of <em>MMP20</em>-associated AI and highlights two critical residues within the MMP20 catalytic domain that are essential for its secretion and enzymatic activity.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 305-314"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunohistochemical evaluation of bone regeneration induced by human umbilical cord mesenchymal stem cells around implants in an osteoporotic rat model 人脐带间充质干细胞在骨质疏松大鼠植入物周围诱导骨再生的免疫组织化学评价

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.08.042

Mefina Kuntjoro , Nike Hendrijantini , Michael Josef Kridanto Kamadjaja , Bambang Agustono Satmoko Tumali , Jennifer Widjaja , Eric Priyo Prasetyo , Guang Hong

Background/purpose

Osteoporosis in the jawbone can compromise the success of dental implant treatment. Human umbilical cord mesenchymal stem cells (hUCMSCs) have demonstrated osteogenic differentiation potential. This study aimed to evaluate the effect of hUCMSC induction on implant osseointegration in an osteoporotic animal model.

Materials and methods

Twenty-eight osteoporotic female Wistar rats were divided into control and hUCMSC-induced groups and observed at 2 and 4 weeks. The treatment group received hUCMSC injections into the implant area of the femur osteoporotic model. Specimens were stained using immunohistochemical and haematoxylin and eosin techniques to assess osteogenic marker expression. Data were analysed using Kruskal–Wallis and Mann–Whitney U test.

Results

Bone-implant volume (BIV) was significantly greater in the hUCMSC-induced groups compared to controls. The expression of osterix, RUNX family transcription factor 2 (RUNX2), tumor necrosis factor (TNF)-α, nuclear factor of activated T cells 1 (NFATc1), collagen type 1, and osteocalcin decreased in osteoblasts from week 2 to week 4. Significant differences (P < 0.05) were observed between control and hUCMSC groups at both time points. These findings suggest that bone formation was completed by week 4, entering the bone maturation phase, supported by the increased BIV in the hUCMSC group.

Conclusion

Induction with hUCMSCs promotes both early and late osseointegration in osteoporotic animal models. These results highlight the efficacy of hUCMSCs in enhancing bone healing after implant placement under osteoporotic conditions.

背景/目的颌骨骨质疏松会影响种植牙治疗的成功。人脐带间充质干细胞（hUCMSCs）已显示出成骨分化潜力。本研究旨在评价hUCMSC诱导对骨质疏松动物模型种植体骨整合的影响。材料与方法将28只骨质疏松雌性Wistar大鼠分为对照组和hucmsc诱导组，分别于2周和4周进行观察。治疗组在股骨骨质疏松模型植入区注射hUCMSC。采用免疫组织化学染色、血红素和伊红染色技术对标本进行染色，以评估成骨标志物的表达。数据分析采用Kruskal-Wallis和Mann-Whitney U检验。结果hucmsc诱导组骨种植体体积（BIV）明显高于对照组。成骨细胞中骨甾、RUNX家族转录因子2 （RUNX2）、肿瘤坏死因子(TNF)-α、活化T细胞核因子1 （NFATc1）、1型胶原和骨钙素的表达在第2周至第4周下降。在两个时间点，对照组和hUCMSC组之间存在显著差异（P < 0.05）。这些结果表明，在hUCMSC组中，骨形成在第4周完成，进入骨成熟阶段，这得到了BIV增加的支持。结论在骨质疏松动物模型中，hUCMSCs诱导可促进早期和晚期骨整合。这些结果强调了hUCMSCs在骨质疏松条件下促进种植体植入后骨愈合的功效。

{"title":"Immunohistochemical evaluation of bone regeneration induced by human umbilical cord mesenchymal stem cells around implants in an osteoporotic rat model","authors":"Mefina Kuntjoro , Nike Hendrijantini , Michael Josef Kridanto Kamadjaja , Bambang Agustono Satmoko Tumali , Jennifer Widjaja , Eric Priyo Prasetyo , Guang Hong","doi":"10.1016/j.jds.2025.08.042","DOIUrl":"10.1016/j.jds.2025.08.042","url":null,"abstract":"<div><h3>Background/purpose</h3><div>Osteoporosis in the jawbone can compromise the success of dental implant treatment. Human umbilical cord mesenchymal stem cells (hUCMSCs) have demonstrated osteogenic differentiation potential. This study aimed to evaluate the effect of hUCMSC induction on implant osseointegration in an osteoporotic animal model.</div></div><div><h3>Materials and methods</h3><div>Twenty-eight osteoporotic female Wistar rats were divided into control and hUCMSC-induced groups and observed at 2 and 4 weeks. The treatment group received hUCMSC injections into the implant area of the femur osteoporotic model. Specimens were stained using immunohistochemical and haematoxylin and eosin techniques to assess osteogenic marker expression. Data were analysed using Kruskal–Wallis and Mann–Whitney U test.</div></div><div><h3>Results</h3><div>Bone-implant volume (BIV) was significantly greater in the hUCMSC-induced groups compared to controls. The expression of osterix, RUNX family transcription factor 2 (RUNX2), tumor necrosis factor (TNF)-α, nuclear factor of activated T cells 1 (NFATc1), collagen type 1, and osteocalcin decreased in osteoblasts from week 2 to week 4. Significant differences (<em>P</em> < 0.05) were observed between control and hUCMSC groups at both time points. These findings suggest that bone formation was completed by week 4, entering the bone maturation phase, supported by the increased BIV in the hUCMSC group.</div></div><div><h3>Conclusion</h3><div>Induction with hUCMSCs promotes both early and late osseointegration in osteoporotic animal models. These results highlight the efficacy of hUCMSCs in enhancing bone healing after implant placement under osteoporotic conditions.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 294-304"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumor microenvironment-derived IL-32 promotes aggressive phenotypes and stem cell traits in head and neck squamous cell carcinoma 肿瘤微环境来源的IL-32促进头颈部鳞状细胞癌的侵袭性表型和干细胞特征

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.10.010

Nien-Tzu Liu , Shin-Hsien Yang , Yi-Ming Chang , Jian-Hong Yu , Su-Feng Chen , Yaoh-Shiang Lin , Yu-Chun Lin

Background/purpose

Head and neck squamous cell carcinoma (HNSCC) is an aggressive malignancy distinguished by marked invasiveness, a high metastatic propensity, and poor prognosis. Cancer-associated fibroblasts (CAFs) within the tumour microenvironment secrete numerous mediators that accelerate tumour progression; however, the precise contribution of CAF-derived interleukin-32 (IL-32) remains unclear. This study examined the influence of CAF-derived IL-32 on invasion, epithelial–mesenchymal transition (EMT), and cancer-stem-cell (CSC) traits in HNSCC.

Materials and methods

Primary CAFs and normal fibroblasts (NFs) were isolated from HNSCC specimens. IL-32 expression was quantified by microarray analysis, quantitative PCR, Western blotting, and enzyme-linked immunosorbent assay. Migration and invasion of FaDu and SCC25 cells were assessed with Transwell assays after exposure to CAF-conditioned medium or recombinant IL-32. EMT markers were evaluated by Western blotting, whereas sphere-formation assays and flow cytometry for CD133⁺/CD44⁺/CD24⁺ populations were used to determine stemness.

Results

IL-32 was significantly up-regulated in CAFs compared with NFs. Both CAF-conditioned medium and recombinant IL-32 markedly increased the migratory and invasive capacities of HNSCC cells. These treatments reduced E-cadherin and increased Vimentin, Snail, and Twist expression, while enhancing sphere formation and expanding CD24⁺, CD44⁺ and CD133⁺ sub-populations.

Conclusion

CAFs promote HNSCC progression through IL-32-mediated enhancement of invasion, EMT induction, and CSC properties. Targeting IL-32 signalling may represent a promising therapeutic approach to improve outcomes in HNSCC.

背景/目的头颈部鳞状细胞癌（HNSCC）是一种侵袭性恶性肿瘤，具有明显的侵袭性、高转移倾向和预后差。肿瘤微环境中的癌症相关成纤维细胞（CAFs）分泌许多加速肿瘤进展的介质；然而，cafc衍生的白介素-32 （IL-32）的确切作用尚不清楚。本研究检测了caf来源的IL-32对HNSCC侵袭、上皮-间质转化（EMT）和癌症干细胞（CSC）性状的影响。材料和方法从HNSCC中分离原代CAFs和正常成纤维细胞（NFs）。通过微阵列分析、定量PCR、Western blotting和酶联免疫吸附法测定IL-32的表达。暴露于cafo培养基或重组IL-32后，用Transwell法评估FaDu和SCC25细胞的迁移和侵袭。EMT标记物采用Western blotting进行评估，而CD133+/CD44+/CD24+群体的球形成试验和流式细胞术用于测定干性。结果与NFs相比，CAFs中sil -32表达明显上调。ca -条件培养基和重组IL-32均能显著提高HNSCC细胞的迁移和侵袭能力。这些处理降低了E-cadherin的表达，增加了Vimentin、Snail和Twist的表达，同时增强了球的形成，扩大了CD24+、CD44+和CD133+亚群。结论：cafs通过il -32介导的侵袭增强、EMT诱导和CSC特性促进HNSCC进展。靶向IL-32信号可能是改善HNSCC预后的一种有希望的治疗方法。

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引用次数: 0

Effects of restorative materials, colors, and surface finishing on the scanning accuracy of an intraoral scanner 修复材料、颜色和表面处理对口腔内扫描仪扫描精度的影响

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.10.040

Sophia Lin , Wei-Chun Lin , Amal Alfaraj

Background/purpose

When teeth are restored using dental materials, there is potential for these restorations to influence the accuracy of intraoral scans. Such variations in accuracy could subsequently affect the precision of the derived virtual cast, the registration of the maxillo-mandibular relationship, and the fabrication of prostheses. This study aimed to assess the effect of various restorative materials, prosthesis colors, and their surface finishes on the accuracy of intraoral scans performed with a TRIOS 4 scanner.

Materials and methods

Using a 4 × 2 × 2 factorial experimental design, the research analyzed how metal-ceramic, zirconia, lithium disilicate, and milled PMMA; shades B1 and A4; and surface finishes (either polishing or glazing) influence the trueness and precision of scans. The trueness and precision were quantified using root mean square (RMS) values.

Results

Significant differences in scan accuracy were observed, contingent on material, color, and finishing, along with notable interactions between these factors. Overall, PMMA exhibited the highest trueness and zirconia demonstrated the best precision. Polished lithium disilicate and PMMA showed better trueness for the A4 shade, whereas glazed lithium disilicate and PMMA performed better for the B1 shade. Metal-ceramic restorations showed an opposite trend. Zirconia restorations showed better trueness for A4 shade than B1 shade for both polished and glazed surfaces.

Conclusion

Significant interactions between materials, colors, and surface treatments were observed. Although clinicians may not be able to modify existing restorations' materials, color, or surface treatment, they need to be mindful that the intricate interaction of these factors will affect the accuracy of intraoral scans.

背景/目的当使用牙科材料修复牙齿时，这些修复体有可能影响口腔内扫描的准确性。这种精度的变化可能随后影响衍生虚拟模型的精度，上颌关系的注册，以及假体的制造。本研究旨在评估各种修复材料、假体颜色及其表面光洁度对TRIOS 4扫描仪口腔内扫描准确性的影响。材料与方法采用4 × 2 × 2因子试验设计，研究了金属陶瓷、氧化锆、二硅酸锂和磨粒PMMA；B1和A4色块；表面处理（抛光或上光）会影响扫描的准确性和精度。用均方根（RMS）值量化真度和精密度。结果观察到扫描精度的显着差异，取决于材料，颜色和整理，以及这些因素之间显着的相互作用。总体而言，PMMA的准确率最高，而氧化锆的准确率最高。抛光后的二硅酸锂和PMMA对A4遮光膜的真性较好，而抛光后的二硅酸锂和PMMA对B1遮光膜的真性较好。金属陶瓷修复体呈现相反的趋势。对于抛光表面和釉面表面，氧化锆修复显示A4阴影比B1阴影的真实度更好。结论材料、颜色和表面处理之间存在显著的相互作用。尽管临床医生可能无法修改现有修复体的材料、颜色或表面处理，但他们需要注意这些因素的复杂相互作用将影响口内扫描的准确性。

{"title":"Effects of restorative materials, colors, and surface finishing on the scanning accuracy of an intraoral scanner","authors":"Sophia Lin , Wei-Chun Lin , Amal Alfaraj","doi":"10.1016/j.jds.2025.10.040","DOIUrl":"10.1016/j.jds.2025.10.040","url":null,"abstract":"<div><h3>Background/purpose</h3><div>When teeth are restored using dental materials, there is potential for these restorations to influence the accuracy of intraoral scans. Such variations in accuracy could subsequently affect the precision of the derived virtual cast, the registration of the maxillo-mandibular relationship, and the fabrication of prostheses. This study aimed to assess the effect of various restorative materials, prosthesis colors, and their surface finishes on the accuracy of intraoral scans performed with a TRIOS 4 scanner.</div></div><div><h3>Materials and methods</h3><div>Using a 4 × 2 × 2 factorial experimental design, the research analyzed how metal-ceramic, zirconia, lithium disilicate, and milled PMMA; shades B1 and A4; and surface finishes (either polishing or glazing) influence the trueness and precision of scans. The trueness and precision were quantified using root mean square (RMS) values.</div></div><div><h3>Results</h3><div>Significant differences in scan accuracy were observed, contingent on material, color, and finishing, along with notable interactions between these factors. Overall, PMMA exhibited the highest trueness and zirconia demonstrated the best precision. Polished lithium disilicate and PMMA showed better trueness for the A4 shade, whereas glazed lithium disilicate and PMMA performed better for the B1 shade. Metal-ceramic restorations showed an opposite trend. Zirconia restorations showed better trueness for A4 shade than B1 shade for both polished and glazed surfaces.</div></div><div><h3>Conclusion</h3><div>Significant interactions between materials, colors, and surface treatments were observed. Although clinicians may not be able to modify existing restorations' materials, color, or surface treatment, they need to be mindful that the intricate interaction of these factors will affect the accuracy of intraoral scans.</div></div>","PeriodicalId":15583,"journal":{"name":"Journal of Dental Sciences","volume":"21 1","pages":"Pages 533-540"},"PeriodicalIF":3.1,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145904189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A scientometric and comparative study of rhabdomyosarcoma research by pediatricians and stomatologists 儿科医生和口腔医生横纹肌肉瘤研究的科学计量学和比较研究

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.09.019

Wenke Chen , Hui Li , Zhenhu Ren , Chongfang Zhang

Background/purpose

Rhabdomyosarcoma (RMS) frequently affects pediatric patients and common occurs in the head and neck region. The purpose of this study was to compare the scientometric characteristics of RMS publications by pediatricians and stomatologists.

Materials and methods

All the papers on RMS were comprehensively retrieved from the Scopus database, and divided into pediatricians and stomatologists groups.

Results

There were 2211 and 234 papers on RMS were published by rheumatologists and stomatologists, respectively. The total citation count was 63,868 and the h index was 111 for RMS publications by pediatricians, while the respective values for stomatologists were 2952 and 29. The two major subtypes of RMS are embryonal and alveolar types, and genetic alterations further categorize RMS into PAX3/7–FOXO1 fusion-positive and fusion-negative types. Cancer chemotherapy mainly vincristine, dactinomycin, and cyclophosphamide, surgery, radiotherapy, and brachytherapy were the common keywords of treatment. The more common keywords such as age, facial neoplasms, mouth neoplasms, orbital neoplasms, gingiva, mandible, DNA-binding proteins, gene silencing, and Rh30 cell line were reported by stomatologists.

Conclusion

This study is the first comprehensive report of the scientometric characteristics of RMS publications by pediatricians and stomatologists, highlighting the need for increased awareness among clinicians to avoid diagnostic delays and ensure timely treatment.

背景/目的habdomyosarcoma （RMS）常见于儿科患者，多发生在头颈部。本研究的目的是比较儿科医生和口腔医生发表的RMS出版物的科学计量学特征。材料与方法综合检索Scopus数据库中有关RMS的论文，分为儿科组和口腔科组。结果风湿病医师发表RMS论文2211篇，口腔医师发表RMS论文234篇。儿科医生的RMS出版物的总引用数为63868，h指数为111，而口腔科医生的RMS出版物的h指数分别为2952和29。RMS的两种主要亚型为胚胎型和肺泡型，基因改变进一步将RMS分为PAX3/7-FOXO1融合阳性和融合阴性。肿瘤化疗以长春新碱、放线菌素、环磷酰胺为主，手术、放疗、近距离放疗是常见的治疗关键词。较为常见的关键词有年龄、面部肿瘤、口腔肿瘤、眼眶肿瘤、牙龈、下颌骨、dna结合蛋白、基因沉默、Rh30细胞系等。结论本研究首次全面报道了儿科医生和口腔医生发表的RMS出版物的科学计量学特征，强调了临床医生需要提高认识，以避免诊断延误并确保及时治疗。

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引用次数: 0

Complicated management of a surgical site infection following open reduction and internal fixation of a mandibular condylar fracture in a patient with multiple antibiotic allergies 1例多重抗生素过敏患者下颌髁骨折切开复位内固定术后手术部位感染的复杂处理

IF 3.1 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Dental Sciences

Pub Date : 2026-01-01 DOI: 10.1016/j.jds.2025.09.004

Keigo Maeda, Shinsuke Yamamoto, Naoki Taniike

引用次数: 0