Diversity of E. faecalis isolated from different clinical specimens by using RAPD-PCR in Hilla Province, Iraq

Abstract

Objectives: To use RAPD analysis to rapidly detects genomic polymorphisms. Methodology: Diagnosis and genotyping of E. faecalis by using specific primer. Results: out of the 105 specimens, 70 (66.6%) had a positive bacterial culture. The remaining 35 (33.3%) showed no growth. Out 70 positive samples, 15 (14.2%) cultured on chromogenic ager medium were found to be E. faecalis. Following the molecular detection method use of a specific primer based on the D-alanine D-alanine ligase gene as a genetic marker for E. faecalis by PCR, the results showed that all isolates were positive for ddlE. RAPD-PCR was used to determine the relationships between 15 E. faecalis isolates. M13 primer shown polymorphism in the isolates tested, yielding 7-14 bands with sizes ranging from 95 to 3000 bp. Two clusters formed the cladogram, which divided the 15 isolates. The first cluster (A) included one isolate, whereas the second cluster (B) contained 14 isolates. Conclusion: The RAPD analysis indicated a clonal dissemination among most of the selected enterococcal isolates, which suggested they belonged to the same isolate