Toxicity Test of The Extract of Pepper Elder (Peperomia pellucida L. Kunth) Using Brine Shrimp Lethality Test (BSLT).

Abstract

Pepper Elder (Peperomia pellucida L. Kunth) is one of the herbal plants that has the potential as an anticancer. Pepper Elder is a weed plant that grows wild in wet and humid environments but is rarely cultivated. Research on the Pepper Elder plant aims to determine the metabolite compounds contained in the plant, its potential as an anticancer, and the LC50 value. The research was conducted using an experimental method with the Brine Shrimp Lethality Test (BSLT) method. Extraction was carried out by maceration method to extract secondary metabolites. Through screening tests it is known that Pepper Elder contains alkaloids, flavonoids, tannins, saponins, and steroids. Tests were carried out with Artemia saline L. larvae at concentrations of 1000 µg/mL, 100 µg/mL, 10 µg/mL, 1 µg/mL with the potency obtained at the toxic level and LC50 value is 40.947 µg/mL.