Isolation of specific antigens from Angiostrongylus cantonensis. 2. By affinity chromatography.

W W Kum, R C Ko
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引用次数: 8

Abstract

The crude adult worm extract of Angiostrongylus cantonensis was subjected to a series of affinity chromatography for selective removal of host antigens as well as cross-reactive components with other helminths. The purified fraction designated as AC(p) with molecular weights ranging from 10,000-42,000 was found to contain antigenic components specific to A. cantonensis as determined by immunoelectrophoresis and enzyme-linked immunosorbent assay.

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广州管圆线虫特异性抗原的分离。2. 亲和色谱法。
对广州管圆线虫成虫粗提物进行亲和层析,选择性去除宿主抗原及与其他线虫的交叉反应成分。经免疫电泳和酶联免疫吸附测定,纯化后的AC(p)分子量在10000 ~ 42000之间,含有广东棘球绦虫特异性抗原成分。
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Studies on the sequence of variable antigen types in ponies infected with a clone of Trypanosoma evansi. Microscopic and flow cytophotometric analysis of parasitemia in cultures of Plasmodium falciparum vitally stained with Hoechst 33342--application to studies of antimalarial agents. Trypanosoma cruzi: inhibition of host cell uptake of infective bloodstream forms by alpha-2-macroglobulin. Species-specific monoclonal antibodies for a membrane antigen(s) in all developmental forms of Trypanosoma cruzi. Isolation of specific antigens from Angiostrongylus cantonensis. 2. By affinity chromatography.
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