In Vitro Comparative Evaluation of the Efficacy of Antimicrobial Photodynamic Therapy, Chlorhexidine, Sodium Fluoride, and Hydrogen Peroxide for Acrylic Resin Disinfection

IF 1.4 Q3 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING Journal of lasers in medical sciences Pub Date : 2023-11-04 DOI:10.34172/jlms.2023.52
Farhad Sobouti, Sepideh Dadgar, Aysan Salehi, Nika Rezaei Kalantari, Mehdi Aryana, Hamid Reza Goli, Mohadeseh Heidari
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Abstract

Introduction: Plaque accumulation on the surface of removable orthodontic appliances could lead to dental caries, periodontitis, and fungal infections. This study evaluated the effectiveness of antimicrobial photodynamic therapy (aPDT), chlorhexidine (CHX), sodium fluoride (NaF), and hydrogen peroxide (H2 O2 ) for the disinfection of acrylic resin. Methods: In this in vitro experimental study, 100 acrylic resin specimens were randomly divided into five groups (n=20 each): Enterococcus faecalis, Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, and Lactobacillus acidophilus. Each group was immersed separately in 5 mL of microbial suspension. They were then incubated until biofilm formation on their surface. Of each microorganism, one biofilm sample in phosphate-buffered saline was considered as negative control, and other biofilm samples (n=80) were subjected to aPDT with curcumin, 0.12% CHX (positive control), 1% H2 O2 , and 0.2% NaF. Finally, the number of colonies was counted. Data were analyzed by the Kruskal-Wallis and Mann-Whitney tests, two-way ANOVA, and Bonferroni adjustment at a significance level of 0.05. Results: The interaction effect of the treatment modality and type of microorganism was significant on the microbial count (effect size: 0.91, P<0.05). Maximum bacterial proliferation was noted in the following combinations: NaF/E. faecalis, H2 O2 /E. faecalis, and H2 O2 /S. salivarius. Microorganisms had no or insignificant growth and proliferation in the aPDT and CHX groups. Conclusion: The results supported the optimal antimicrobial efficacy of PDT which was comparable to that of CHX. aPDT showed superior antimicrobial efficacy to NaF and H2 O2 for the disinfection of acrylic resin.
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抗菌光动力疗法、氯己定、氟化钠和过氧化氢对丙烯酸树脂消毒效果的体外比较评价
牙菌斑在可移动的正畸矫治器表面堆积会导致龋齿、牙周炎和真菌感染。本研究评价了抗菌光动力疗法(aPDT)、氯己定(CHX)、氟化钠(NaF)和过氧化氢(H2 O2)对丙烯酸树脂的消毒效果。方法:采用体外实验方法,将100份丙烯酸树脂标本随机分为粪肠球菌、变形链球菌、唾液链球菌、血链球菌和嗜酸乳杆菌5组,每组20例。各组分别浸于5 mL微生物悬液中。然后将它们孵育至表面形成生物膜。每一种微生物取一份生物膜样品作为阴性对照,其余生物膜样品(n=80)分别用姜黄素、0.12% CHX(阳性对照)、1% H2 O2和0.2% NaF进行aPDT处理。最后,计算殖民地的数量。数据分析采用Kruskal-Wallis检验和Mann-Whitney检验、双向方差分析和Bonferroni调整,显著性水平为0.05。结果:处理方式和微生物类型对微生物数量的交互作用显著(效应值:0.91,P<0.05)。NaF/E组合中细菌增殖量最大。粪,H2 O2 /E。和H2 O2 /S。了唾液。aPDT组和CHX组微生物没有或不显著生长和增殖。结论:PDT具有与CHX相当的最佳抑菌效果。aPDT对丙烯酸树脂的消毒效果优于NaF和H2 O2。
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来源期刊
Journal of lasers in medical sciences
Journal of lasers in medical sciences RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-
CiteScore
3.00
自引率
13.30%
发文量
24
期刊介绍: The "Journal of Lasers in Medical Sciences " is a scientific quarterly publication of the Laser Application in Medical Sciences Research Center, Shahid Beheshti University of Medical Sciences. This journal received a scientific and research rank from the national medical publication committee. This Journal accepts original papers, review articles, case reports, brief reports, case series, photo assays, letters to the editor, and commentaries in the field of laser, or light in any fields of medicine such as the following medical specialties: -Dermatology -General and Vascular Surgery -Oncology -Cardiology -Dentistry -Urology -Rehabilitation -Ophthalmology -Otorhinolaryngology -Gynecology & Obstetrics -Internal Medicine -Orthopedics -Neurosurgery -Radiology -Pain Medicine (Algology) -Basic Sciences (Stem cell, Cellular and Molecular application and physic)
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