{"title":"Use of gamma radiation for the antioxidant improvement of saffron [<i>Crocus sativus</i>] petal extract","authors":"Marzieh Heidarieh, Mahnoosh Parsaeimehr, Najmeh Sheikhzadeh","doi":"10.1515/ract-2023-0188","DOIUrl":null,"url":null,"abstract":"Abstract This in vitro study was conducted to evaluate the impact of gamma-irradiation on the antioxidant activity of the methanolic extracts of Crocus sativus petals. After gamma-irradiation at 5, 10, and 35 kGy doses, Folin-Ciocalteu , aluminum chloride colorimetric methods, Fourier transform infrared spectroscopy (FTIR), and high performance liquid chromatography (HPLC) techniques were deployed to analyze the irradiated and non-irradiated extracts. The obtained results were indicative of boosted levels of phenolic and total flavonoids compounds in methanolic extracts irradiated at 5 and 10 kGy. Results of the HPLC method also revealed that gamma-irradiation, especially at 5 kGy, could increase the quantity of certain polyphenols, including chlorogenic acid, epicatechin caffeic acid, and thioxanthene, in the saffron extract. In contrast, gamma-irradiation at 35 kGy exerted certain adverse effects on polyphenols contents. In conclusion, gamma-irradiation, especially at 5 kGy, could enhance the antioxidant potential of the methanolic extract of saffron petals.","PeriodicalId":21167,"journal":{"name":"Radiochimica Acta","volume":"45 1","pages":"0"},"PeriodicalIF":1.4000,"publicationDate":"2023-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Radiochimica Acta","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/ract-2023-0188","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, INORGANIC & NUCLEAR","Score":null,"Total":0}
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Abstract
Abstract This in vitro study was conducted to evaluate the impact of gamma-irradiation on the antioxidant activity of the methanolic extracts of Crocus sativus petals. After gamma-irradiation at 5, 10, and 35 kGy doses, Folin-Ciocalteu , aluminum chloride colorimetric methods, Fourier transform infrared spectroscopy (FTIR), and high performance liquid chromatography (HPLC) techniques were deployed to analyze the irradiated and non-irradiated extracts. The obtained results were indicative of boosted levels of phenolic and total flavonoids compounds in methanolic extracts irradiated at 5 and 10 kGy. Results of the HPLC method also revealed that gamma-irradiation, especially at 5 kGy, could increase the quantity of certain polyphenols, including chlorogenic acid, epicatechin caffeic acid, and thioxanthene, in the saffron extract. In contrast, gamma-irradiation at 35 kGy exerted certain adverse effects on polyphenols contents. In conclusion, gamma-irradiation, especially at 5 kGy, could enhance the antioxidant potential of the methanolic extract of saffron petals.
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