LC-MS/MS investigation of phytochemical ingredients and alpha amylase inhibition activity of Cassia siamea Lam leaves aqueous extracts

Abstract

The main objective of the present investigation study was to analyze the phytochemical ingredients and alpha amylase inhibition activity of aqueous extract of Cassia siamea Lam plant leaves. Phytochemical ingredients analysis was performed by using LC-MS/MS analysis spectral technique and qualitative analysis through the well-known standard tests protocol available in the literature. Also study was aimed to investigate alpha amylase inhibition activity of leaves aqueous extract of Cassia siamea Lam as well established bioactive phytochemical ingredients responsible for such activity by porcine pancreatic alpha amylase enzyme inhibition in vitro assay. The LC-MS/MS analysis of aqueous extract reported the presence of a total 9 phytochemical ingredients in the leaves of Cassia siamea Lam, while phytochemical qualitative analysis of leaves aqueous extract also noted the presence of secondary metabolites like carbohydrates, proteins, amino acids, flavonoids, tannins, glycosides, steroids and phenolic compounds. IC50 values obtained by alpha amylase inhibition activity for Cassia siamea Lamaqueous extract was found to be 30.38 ± 0.03 μg/mL and acarbose as a standard reference was found to be 27.62 ± 0.02 mg/mL. The extract showed promising alpha amylase inhibitionactivity against tested porcine pancreatic alpha amylase enzyme has varying degree of alpha amylase inhibitionactivity ranging from lower to higher dose dependent activity correlated by the presence of bioactive phytochemical ingredients. The results of investigation study suggest that Cassia siamea Lam medicinal plant leaves has promising alpha amylase inhibition activity and could serve as potential source of natural alpha amylase inhibitor as an alternative medicine to treat disorders of diabetes mellitus.