Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00011
Arjun Singh
Traditional medicine is a set of knowledge, abilities, and procedures based on assumptions, beliefs, and experiences of traditional societies to preserve their health. Many rural or indigenous people in many undeveloped nations place a high importance on traditional herbal remedies. Andrographis paniculata Wall (family Acanthaceae) is a common medicinal plant that has been used for millennia in Asia, America, and Africa to treat a variety of maladies including cancer, diabetes, high blood pressure, ulcer, leprosy, bronchitis, skin diseases, flatulence, colic, influenza, dysentery, dyspepsia, and malaria. It contains several photochemical compounds that have distinct and intriguing biological effects. To bridge the gap for future research prospects, this study discusses the past and present state of research on Andrographis paniculata in terms of medicinal usage, phytochemistry, pharmacological activities, toxicity profile, and therapeutic usage. This assessment is based on a review of the literature in scientific journals and books obtained from libraries and electronic sources. Natural therapeutic properties are just one benefit of medicinal plants; they also provide disease prevention. In this concise overview study research, we are trying to summarize, aggregate the number of plants, and identify their ethnopharmacological properties.
{"title":"Ethnopharmacological, Phytochemical, Pharmacognostical, and Clinical significance of Andrographis paniculata (King of bitters): An Overview","authors":"Arjun Singh","doi":"10.52711/0974-4150.2024.00011","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00011","url":null,"abstract":"Traditional medicine is a set of knowledge, abilities, and procedures based on assumptions, beliefs, and experiences of traditional societies to preserve their health. Many rural or indigenous people in many undeveloped nations place a high importance on traditional herbal remedies. Andrographis paniculata Wall (family Acanthaceae) is a common medicinal plant that has been used for millennia in Asia, America, and Africa to treat a variety of maladies including cancer, diabetes, high blood pressure, ulcer, leprosy, bronchitis, skin diseases, flatulence, colic, influenza, dysentery, dyspepsia, and malaria. It contains several photochemical compounds that have distinct and intriguing biological effects. To bridge the gap for future research prospects, this study discusses the past and present state of research on Andrographis paniculata in terms of medicinal usage, phytochemistry, pharmacological activities, toxicity profile, and therapeutic usage. This assessment is based on a review of the literature in scientific journals and books obtained from libraries and electronic sources. Natural therapeutic properties are just one benefit of medicinal plants; they also provide disease prevention. In this concise overview study research, we are trying to summarize, aggregate the number of plants, and identify their ethnopharmacological properties.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140438498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Alzheimer's disease (AD) is a major problem in today's societies. More than five million Americans are living with Alzheimer's disease in the United States, with the majority being 65 and older. According to the Alzheimer's Association Report, the number of persons affected by Alzheimer's disease in the United States would rise to fourteen million by 2060. The disease, which is the most prevalent form of dementia, is a progressive and irreversible brain disorder that gradually deteriorates an individual's cognitive function. It advances from preclinical to early- to moderate- to late-stage disease. Early symptoms primarily include cognitive impairment, particularly memory loss. Current Alzheimer's disease treatment can be divided into two categories based on the disease's stage. Galantamine, rivastigmine, and donepezil as acetylcholinesterase inhibitors are suitable for mild to moderate cases to provide transient symptomatic relief among patients. Memantine, an N-methyl D-aspartate (NMDA) antagonist, is used as monotherapy to treat symptoms of moderate to severe Alzheimer's disease. These medications are typically selective molecules that target certain proteins ("one compound-one target" method), and their main goal is to restore physiological acetylcholine levels. Nonetheless, multiple pathways of Alzheimer's disease pathogenesis have been hypothesized to far, and they have been proven to overlap and influence one another.
{"title":"Biological screening to identify hits the Therapeutic Targets of Alzheimer's disease and their role in the pathogenesis","authors":"Pooja Singh, Divya Sharma, Akanksha Singh, Himanshu Gupta, Arjun Singh","doi":"10.52711/0974-4150.2024.00009","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00009","url":null,"abstract":"Alzheimer's disease (AD) is a major problem in today's societies. More than five million Americans are living with Alzheimer's disease in the United States, with the majority being 65 and older. According to the Alzheimer's Association Report, the number of persons affected by Alzheimer's disease in the United States would rise to fourteen million by 2060. The disease, which is the most prevalent form of dementia, is a progressive and irreversible brain disorder that gradually deteriorates an individual's cognitive function. It advances from preclinical to early- to moderate- to late-stage disease. Early symptoms primarily include cognitive impairment, particularly memory loss. Current Alzheimer's disease treatment can be divided into two categories based on the disease's stage. Galantamine, rivastigmine, and donepezil as acetylcholinesterase inhibitors are suitable for mild to moderate cases to provide transient symptomatic relief among patients. Memantine, an N-methyl D-aspartate (NMDA) antagonist, is used as monotherapy to treat symptoms of moderate to severe Alzheimer's disease. These medications are typically selective molecules that target certain proteins (\"one compound-one target\" method), and their main goal is to restore physiological acetylcholine levels. Nonetheless, multiple pathways of Alzheimer's disease pathogenesis have been hypothesized to far, and they have been proven to overlap and influence one another.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140440376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00008
Avnish Kumar Arora
The synthesis of cadmium oxide nanoparticles has been carried out in the presence of Sapindus mukorossi (Soapnut) as a surfactant and the characterisation of the synthesized nanoparticles has been carried out using analytical techniques such as XRD, magnetic studies and SEM. The synthesised cadmium oxide was CdO. The structure of the CdO was face-centred cubic. Magnetic susceptibility measurements showed that there were no unpaired electrons in CdO. Hence, CdO is diamagnetic in nature. The exact size of the cadmium oxide was found using SEM. The size of the oxide was from 28 nm to 50 nm.
以无患子（Sapindus mukorossi）为表面活性剂合成了纳米氧化镉颗粒，并使用 XRD、磁性研究和 SEM 等分析技术对合成的纳米颗粒进行了表征。合成的氧化镉为 CdO。氧化镉的结构是面心立方体。磁感应强度测量结果表明，氧化镉中不存在未成对电子。因此，氧化镉具有二磁性。利用扫描电子显微镜发现了氧化镉的确切尺寸。氧化镉的尺寸在 28 纳米到 50 纳米之间。
{"title":"Synthesis and Characterization of Cadmium Oxide NPs using Sapindus mukorossi (Soapnut) as natural surfactant","authors":"Avnish Kumar Arora","doi":"10.52711/0974-4150.2024.00008","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00008","url":null,"abstract":"The synthesis of cadmium oxide nanoparticles has been carried out in the presence of Sapindus mukorossi (Soapnut) as a surfactant and the characterisation of the synthesized nanoparticles has been carried out using analytical techniques such as XRD, magnetic studies and SEM. The synthesised cadmium oxide was CdO. The structure of the CdO was face-centred cubic. Magnetic susceptibility measurements showed that there were no unpaired electrons in CdO. Hence, CdO is diamagnetic in nature. The exact size of the cadmium oxide was found using SEM. The size of the oxide was from 28 nm to 50 nm.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140440548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00010
Priyanka Kumari, Divya Sharma, Arjun Singh
Since the beginnings of cancer research, phytochemicals have been a focal point because they were some of the first antineoplastic drugs found (e.g., leucovorin in 1950, carzinophilin in 1954, vincristine in 1963, actinomycin D in 1964, etc.). Additionally, their research is still ongoing today. It is crucial to note that natural substances are used both as adjuvants and chemotherapeutic agents in the treatment of cancer. By summarizing the three aspects of natural compounds' anticancer activity, this review offers a fresh perspective on their use in the field of oncology. Chemotherapeutic drugs because of their inherent antitumor effects, chemopreventive drugs, and sensitizers for multi-drug resistance are the first three categories. Numerous active agents are undergoing preclinical and clinical trials right now. It is crucial to draw attention to the important advancements that have been made in this field of study, with some secondary plant metabolites already in use in medicine and others being tested in human clinical trials as anticancer agents. Phytochemicals have numerous positive effects on human health and are crucial in preventing the development and spread of cancer. Through a diet high in fruits and vegetables, as well as with the aid of highly-standardized supplements containing the active compounds, one may achieve the ideal intake of natural chemopreventive agents.
自癌症研究开始以来，植物化学物质一直是研究的重点，因为它们是最早发现的抗肿瘤药物（如 1950 年的亮菌甲素、1954 年的卡扎菲林、1963 年的长春新碱、1964 年的放线菌素 D 等）。此外，他们的研究至今仍在继续。值得注意的是，天然物质在癌症治疗中既可用作辅助药物，也可用作化疗药物。通过总结天然化合物抗癌活性的三个方面，本综述为天然化合物在肿瘤学领域的应用提供了一个全新的视角。因其固有的抗肿瘤作用而成为化疗药物、化学预防药物和多种药物耐药性的增敏剂是前三类。目前，许多活性药物正在进行临床前和临床试验。一些植物次生代谢物已被用于医疗，另一些则作为抗癌药物正在进行人体临床试验，提请注意这一研究领域取得的重要进展至关重要。植物化学物质对人类健康有许多积极影响，对预防癌症的发展和扩散至关重要。通过多吃水果和蔬菜，以及借助含有活性化合物的高标准补充剂，人们可以达到理想的天然化学预防剂摄入量。
{"title":"In-vivo Studies conducted following the success In-vitro and Dissemination of Anticancer Clinical Trials","authors":"Priyanka Kumari, Divya Sharma, Arjun Singh","doi":"10.52711/0974-4150.2024.00010","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00010","url":null,"abstract":"Since the beginnings of cancer research, phytochemicals have been a focal point because they were some of the first antineoplastic drugs found (e.g., leucovorin in 1950, carzinophilin in 1954, vincristine in 1963, actinomycin D in 1964, etc.). Additionally, their research is still ongoing today. It is crucial to note that natural substances are used both as adjuvants and chemotherapeutic agents in the treatment of cancer. By summarizing the three aspects of natural compounds' anticancer activity, this review offers a fresh perspective on their use in the field of oncology. Chemotherapeutic drugs because of their inherent antitumor effects, chemopreventive drugs, and sensitizers for multi-drug resistance are the first three categories. Numerous active agents are undergoing preclinical and clinical trials right now. It is crucial to draw attention to the important advancements that have been made in this field of study, with some secondary plant metabolites already in use in medicine and others being tested in human clinical trials as anticancer agents. Phytochemicals have numerous positive effects on human health and are crucial in preventing the development and spread of cancer. Through a diet high in fruits and vegetables, as well as with the aid of highly-standardized supplements containing the active compounds, one may achieve the ideal intake of natural chemopreventive agents.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140439489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00005
Perla Ganesh, CH. B. V. Narasimha Raju, N. Jagadeesh, Hemant M. Gandhi, BM. Rao, Dharamasoth Rama Devi, K. Basavaiah
The study aimed to develop and validate the method for trace level analysis of 2-(3-(trifluoromethyl)phenyl)propanal (2-TFL) impurity in calcium sensing receptor drugs by gas chromatographic method using mass spectrometry (GC-MS). The method utilizes a capillary column with 6% cyanopropyl phenyl and 94% dimethylpolysiloxane stationary phase with EI source in Selective Ion Monitoring (SIM) mode under programming temperature was used. After successful development, the method was validated according to our in-house validation guidelines for trace level analysis. The method proved to be selective for 2-(3-(trifluoromethyl) phenyl)propanal impurity in drug substance. A dissolve and injection approach was adopted for sample introduction in a split mode. Methanol was used as a diluent. The calibration curves showed good linearity over the concentration range from 1.85 (LOQ to 5.56ppm (150%)) of the target concentration of 3 ppm. The correlation coefficient obtained was >0.999. A limit of detection (LOD) of about 0.56ppm was achieved and limit of quantitation (LOQ) of 1.85ppm was achieved for 2-(3-(trifluoromethyl)phenyl)propanal impurity, when the samples were prepared at 20mg/mL. While recovery proved to be 101.0% at Limit of quantification (LOQ) level, 102.1% at Target analytical level (TAL) and 98.1% at 150% of the TAL, demonstrate the absence of matrix effect.
{"title":"Trace level Determination of 2-(3-(trifluoromethyl)phenyl)propanal in Calcium Sensing Receptor drug by GCMS","authors":"Perla Ganesh, CH. B. V. Narasimha Raju, N. Jagadeesh, Hemant M. Gandhi, BM. Rao, Dharamasoth Rama Devi, K. Basavaiah","doi":"10.52711/0974-4150.2024.00005","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00005","url":null,"abstract":"The study aimed to develop and validate the method for trace level analysis of 2-(3-(trifluoromethyl)phenyl)propanal (2-TFL) impurity in calcium sensing receptor drugs by gas chromatographic method using mass spectrometry (GC-MS). The method utilizes a capillary column with 6% cyanopropyl phenyl and 94% dimethylpolysiloxane stationary phase with EI source in Selective Ion Monitoring (SIM) mode under programming temperature was used. After successful development, the method was validated according to our in-house validation guidelines for trace level analysis. The method proved to be selective for 2-(3-(trifluoromethyl) phenyl)propanal impurity in drug substance. A dissolve and injection approach was adopted for sample introduction in a split mode. Methanol was used as a diluent. The calibration curves showed good linearity over the concentration range from 1.85 (LOQ to 5.56ppm (150%)) of the target concentration of 3 ppm. The correlation coefficient obtained was >0.999. A limit of detection (LOD) of about 0.56ppm was achieved and limit of quantitation (LOQ) of 1.85ppm was achieved for 2-(3-(trifluoromethyl)phenyl)propanal impurity, when the samples were prepared at 20mg/mL. While recovery proved to be 101.0% at Limit of quantification (LOQ) level, 102.1% at Target analytical level (TAL) and 98.1% at 150% of the TAL, demonstrate the absence of matrix effect.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140439776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00002
Tariqul Islam, Nur Amin Bitu, F. Hossen, A. Asraf, Kudrat E-Zahan
A Schiff base ligand was synthesized via the condensation reaction of thiosemicarbazide and salicylaldehyde. A series of transition metal complexes of VO(IV), Ni(II), Cu(II), and Zn(II) were obtained by the complexation reaction with the synthesized Schiff base ligand. The Schiff base ligand and its metal complexes were characterized by UV-Vis and FTIR spectroscopy, TGA-DTA, and some physical measurements. FTIR spectral studies showed that the NOS donor ligand coordinated to the metal ions through the N of azomethine group, S of thiolate, and O of deprotonated hydroxyl group. Molar conductance values indicated non electrolytic nature of the complexes. Magnetic moment values coupled with electronic spectral data exhibited the geometrical structure of the complexes. The Schiff base and its complexes were exposed to disinfectant studies tested by using the disc diffusion method. All the synthesized compounds exhibited moderate to strong antibacterial activity. The copper complexes showed more antibacterial activity than other complexes and Schiff bases. All the Synthesized complexes showed a good cytotoxic activity.
通过硫代氨基脲和水杨醛的缩合反应合成了一种希夫碱配体。通过与合成的希夫碱配体发生络合反应，得到了一系列 VO(IV)、Ni(II)、Cu(II) 和 Zn(II) 的过渡金属配合物。希夫碱配体及其金属配合物通过紫外-可见光谱、傅里叶变换红外光谱、TGA-DTA 和一些物理测量进行了表征。傅立叶变换红外光谱研究表明，NOS 供体配体通过偶氮甲基的 N、硫酸根的 S 和去质子化羟基的 O 与金属离子配位。摩尔电导值表明复合物具有非电解性质。磁矩值和电子光谱数据显示了配合物的几何结构。利用圆盘扩散法对希夫碱及其配合物进行了消毒研究。所有合成化合物都表现出中等至较强的抗菌活性。铜络合物比其他络合物和席夫碱具有更强的抗菌活性。所有合成的复合物都显示出良好的细胞毒性活性。
{"title":"Investigation on Biological Activities of Thiosemicarbazide Derived Schiff Base-Metal Complexes","authors":"Tariqul Islam, Nur Amin Bitu, F. Hossen, A. Asraf, Kudrat E-Zahan","doi":"10.52711/0974-4150.2024.00002","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00002","url":null,"abstract":"A Schiff base ligand was synthesized via the condensation reaction of thiosemicarbazide and salicylaldehyde. A series of transition metal complexes of VO(IV), Ni(II), Cu(II), and Zn(II) were obtained by the complexation reaction with the synthesized Schiff base ligand. The Schiff base ligand and its metal complexes were characterized by UV-Vis and FTIR spectroscopy, TGA-DTA, and some physical measurements. FTIR spectral studies showed that the NOS donor ligand coordinated to the metal ions through the N of azomethine group, S of thiolate, and O of deprotonated hydroxyl group. Molar conductance values indicated non electrolytic nature of the complexes. Magnetic moment values coupled with electronic spectral data exhibited the geometrical structure of the complexes. The Schiff base and its complexes were exposed to disinfectant studies tested by using the disc diffusion method. All the synthesized compounds exhibited moderate to strong antibacterial activity. The copper complexes showed more antibacterial activity than other complexes and Schiff bases. All the Synthesized complexes showed a good cytotoxic activity.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140441569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00004
Sathish Kumar Mittapalli, J. N. N. Sharath Chandra, Jay Prakash Soni, Ram Babu Tripathi, I. Rizwana
More than 100 nations in Africa, the Americas and the Eastern Mediterranean have endemic dengue and malaria, and these bites have the potential to have inflammatory or infectious local or systemic effects. The mosquito repellents from various sources were examined and plant repellents, researchers frequently exaggerate toxicity of the mentioned compound, alleging that it poses a threat to human health or even the environment and reported by recent studies. The aim of the research article, the historical developmental strategies of synthetics and natural and those acts on certain targets as repellent property, target discovery aims to identify and validate appropriate pharmacological targets for therapeutic interventions and establishment of correlations between the molecule and its potency as repellent using various physicochemical parameters and in silico studies. From this study proved that some of the natural compounds were potent in comparison with the some of the standard insect repellents and the paradigm offers a wide variety of prospects that will fasten the lead molecular modifications influences the novel targets and ultimately result in the optimization of the molecular properties by considering the all the methodologies leads to design novel molecules.
{"title":"Virtual Screening and Pharmacophore Modeling for discovery of Biologically Active Natural Products as Inhibitors of Odorant-binding Proteins","authors":"Sathish Kumar Mittapalli, J. N. N. Sharath Chandra, Jay Prakash Soni, Ram Babu Tripathi, I. Rizwana","doi":"10.52711/0974-4150.2024.00004","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00004","url":null,"abstract":"More than 100 nations in Africa, the Americas and the Eastern Mediterranean have endemic dengue and malaria, and these bites have the potential to have inflammatory or infectious local or systemic effects. The mosquito repellents from various sources were examined and plant repellents, researchers frequently exaggerate toxicity of the mentioned compound, alleging that it poses a threat to human health or even the environment and reported by recent studies. The aim of the research article, the historical developmental strategies of synthetics and natural and those acts on certain targets as repellent property, target discovery aims to identify and validate appropriate pharmacological targets for therapeutic interventions and establishment of correlations between the molecule and its potency as repellent using various physicochemical parameters and in silico studies. From this study proved that some of the natural compounds were potent in comparison with the some of the standard insect repellents and the paradigm offers a wide variety of prospects that will fasten the lead molecular modifications influences the novel targets and ultimately result in the optimization of the molecular properties by considering the all the methodologies leads to design novel molecules.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140441664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00003
Padmashree D, Srinivas M, Pooja D, Hema S, Karigar C S, Krupa S
Acid phosphatase was extracted from Abelmoschus esculentus seeds at different pH levels in various buffers. The enzyme was allowed to react with p-nitrophenylphosphate which showed the highest activity in 100mM acetate buffer, pH 5.0 on the 4th day of germination. While the protein was found to be high on the 6th day. The protein content declined from the 11tn day whereas the content remained constant from the 18th day onward. The enzyme showed maximum activity while subjected to a temperature of 550C and pH 5.0, respectively. The enzyme was thermostable at 500C - 600C and pH stable at 4.0 - 5.5. The Km and Vmax values for pNPP were determined as 0.27mM and 9.09 micromoles/min respectively. In the present work, standardization of the kinetic parameters has been performed for achieving the purification and characterization of acid phosphatase which are currently underway.
{"title":"Extraction and Standardisation of Acid Phosphatase from the seeds of Abelmoschus esculentus (Okra)","authors":"Padmashree D, Srinivas M, Pooja D, Hema S, Karigar C S, Krupa S","doi":"10.52711/0974-4150.2024.00003","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00003","url":null,"abstract":"Acid phosphatase was extracted from Abelmoschus esculentus seeds at different pH levels in various buffers. The enzyme was allowed to react with p-nitrophenylphosphate which showed the highest activity in 100mM acetate buffer, pH 5.0 on the 4th day of germination. While the protein was found to be high on the 6th day. The protein content declined from the 11tn day whereas the content remained constant from the 18th day onward. The enzyme showed maximum activity while subjected to a temperature of 550C and pH 5.0, respectively. The enzyme was thermostable at 500C - 600C and pH stable at 4.0 - 5.5. The Km and Vmax values for pNPP were determined as 0.27mM and 9.09 micromoles/min respectively. In the present work, standardization of the kinetic parameters has been performed for achieving the purification and characterization of acid phosphatase which are currently underway.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140439003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00007
Pramod Kumar, Aman Kapoor, Abhishek Kumar, V. K. Gupta
As we know fresh water is a finite and limited resource on Earth and, increasingly is polluted, by both pathogenic microbes and chemical contaminants. Human demand for freshwater is increasing water is required to irrigate crops to feed the rapidly expanding human population. Our main focus during this work is to identify impurities present in Manikaran in terms of Analytical Chemistry which include Limit Tests of Heavy metals. Limit test is best method for detection of impurities present and determination of heavy metals. It is done for identify that the Manikaran water is safe for human use or not. After the completion of the work, it was found that use of water of these resources contains Sulphur as heavy metal.
{"title":"Quantitative Determination of Sulphur in Manikaran’s Water","authors":"Pramod Kumar, Aman Kapoor, Abhishek Kumar, V. K. Gupta","doi":"10.52711/0974-4150.2024.00007","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00007","url":null,"abstract":"As we know fresh water is a finite and limited resource on Earth and, increasingly is polluted, by both pathogenic microbes and chemical contaminants. Human demand for freshwater is increasing water is required to irrigate crops to feed the rapidly expanding human population. Our main focus during this work is to identify impurities present in Manikaran in terms of Analytical Chemistry which include Limit Tests of Heavy metals. Limit test is best method for detection of impurities present and determination of heavy metals. It is done for identify that the Manikaran water is safe for human use or not. After the completion of the work, it was found that use of water of these resources contains Sulphur as heavy metal.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140440249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-02-22DOI: 10.52711/0974-4150.2024.00006
Yougoubo Abdoulaye, D. Constantin M., Soré Harouna, Bationo K. Rémy, Ganame Arouna, Sawadogo Assétou, Roamba Noëlle Edwige, Bazié Benjamin, Kabore S. Dominique, Koala Moumouni, Palé Eloi, Nebie C. H. Roger, N. Mouhoussine
Effective management of malaria remains a major concern for the scientific community mainly due to the resistance of Plasmodium falciparum to the common antimalarials. Many studies are conducted to identify plants that can fight against this disease. This work aimed to contribute to a better knowledge of antiplasmodial activity of different parts of Grangea maderaspatana (Asteracea). Thus, roots, flowers, leafy branches and whole plant of Grangea maderaspatana were extracted with hexane, dichloromethane, ethyl acetate and methanol. Alkaloid contents and antiplasmodial activity of extracts were respectively determined by bromocresol green method and enzyme-linked immunosorbent assay based on pLDH quantification. Total alkaloid contents ranged from 21.036 in methanolic extract of leafy branches to 1074.752µg qE/g in dichloromethane extract of whole plant. Extracts showed moderate to very active antiplasmodial effects. Antiplasmodial activity evaluated on chloroquino (CQ)-sensitive D10 and CQ-resistant Dd2 strains of Plasmodium falciparum showed that hexane and dichloromethane leafy branches extracts had a better activity. Alkaloid could be potentially responsible for antiplasmodial activity observed. None extract found to be toxic for cells at 50 and 100µg/ml. Dichloromethane extract of leafy branches could be a potential source of antiplasmodial molecules.
{"title":"Total Alkaloid content and In vitro Antiplasmodial activity of Grangea maderaspatana collected from Burkina Faso","authors":"Yougoubo Abdoulaye, D. Constantin M., Soré Harouna, Bationo K. Rémy, Ganame Arouna, Sawadogo Assétou, Roamba Noëlle Edwige, Bazié Benjamin, Kabore S. Dominique, Koala Moumouni, Palé Eloi, Nebie C. H. Roger, N. Mouhoussine","doi":"10.52711/0974-4150.2024.00006","DOIUrl":"https://doi.org/10.52711/0974-4150.2024.00006","url":null,"abstract":"Effective management of malaria remains a major concern for the scientific community mainly due to the resistance of Plasmodium falciparum to the common antimalarials. Many studies are conducted to identify plants that can fight against this disease. This work aimed to contribute to a better knowledge of antiplasmodial activity of different parts of Grangea maderaspatana (Asteracea). Thus, roots, flowers, leafy branches and whole plant of Grangea maderaspatana were extracted with hexane, dichloromethane, ethyl acetate and methanol. Alkaloid contents and antiplasmodial activity of extracts were respectively determined by bromocresol green method and enzyme-linked immunosorbent assay based on pLDH quantification. Total alkaloid contents ranged from 21.036 in methanolic extract of leafy branches to 1074.752µg qE/g in dichloromethane extract of whole plant. Extracts showed moderate to very active antiplasmodial effects. Antiplasmodial activity evaluated on chloroquino (CQ)-sensitive D10 and CQ-resistant Dd2 strains of Plasmodium falciparum showed that hexane and dichloromethane leafy branches extracts had a better activity. Alkaloid could be potentially responsible for antiplasmodial activity observed. None extract found to be toxic for cells at 50 and 100µg/ml. Dichloromethane extract of leafy branches could be a potential source of antiplasmodial molecules.","PeriodicalId":8550,"journal":{"name":"Asian Journal of Research in Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140441694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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