A public mid-density genotyping platform for alfalfa (Medicago sativa L.)

Dongyan Zhao, Katherine Maria Mejia-Guerra, Marcelo Mollinari, Deborah Samac, Brian Irish, Katarzyna Heller-Uszynska, Craig Thomas Beil, Moira Jane Sheehan
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Abstract

Small public breeding programmes have many barriers to adopting technology, particularly creating and using genetic marker panels for genomic-based decisions in selection. Here we report the creation of a DArTag panel of 3,000 loci distributed across the alfalfa (Medicago sativa L.) genome for use in molecular breeding and genomic insight. The creation of this marker panel brings cost-effective and rapid genotyping capabilities to alfalfa breeding programmes. The open access provided by this platform will allow genetic data sets generated on the marker panel to be compared and joined across projects, institutions and countries. This genotyping resource has the power to make routine genotyping a reality for any breeder of alfalfa.
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紫花苜蓿(Medicago sativa L.)中等密度基因分型公共平台
小型公共育种计划在采用技术方面存在许多障碍,特别是在基于基因组的选择决策中创建和使用遗传标记面板。在这里,我们报告了分布在苜蓿(Medicago sativa L.)基因组中的3000个位点的DArTag面板的创建,用于分子育种和基因组洞察。该标记组的创建为苜蓿育种规划带来了经济高效和快速的基因分型能力。该平台提供的开放访问将允许在标记面板上生成的遗传数据集在项目、机构和国家之间进行比较和连接。这种基因分型资源有能力使常规基因分型成为现实的任何育种紫花苜蓿。
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