Proteome-wide Profiling Reveals Molecular Mechanism Underlying the Therapeutic Effect of Mongolian Medicine Sulongga-4 on Pyloric Ligationinduced Gastroduodenal Ulcer in Rats

Abstract

background: Gastroduodenal ulcer is one of the common global gastrointestinal diseases. Sulongga-4 is a traditional Mongolian medicine prescription used for the treatment of gastroduodenal ulcer. However, molecular mechanism underlying the therapeutic effect of Sulongga-4 on gastroduodenal ulcer in Proteome has not been clarified. objective: Explore the molecular mechanism behind the therapeutic efficacy of traditional Mongolian medicine Sulongga-4 on the pyloric ligation-induced gastroduodenal ulcer in rats method: Gastroduodenal was induced by pyloric ligation in rats. The pathological changes of gastric and duodenal tissues were observed first, and then the serum levels of AST and ALT were measured. The significantly different proteins were verified by western blot and qRT-PCR. result: The proteomics results showed that the Mongolian medicine Sulongga-4 might act on pyloric ligation-induced gastroduodenal ulcer through differentially expressing several proteins including RPL35, RPL37 and LOC102548628 in gastric tissue, as well as Serpin b1a, Serpin b6a and Vtn in duodenal tissue via regulating ribosome, alcoholism and amoebiasis, complement and coagulation cascades pathways. In addition, the changes of serum AST and ALT levels in rats showed that gastroduodenal ulcer was associated with liver injury. conclusion: Sulongga-4 shows robust therapeutic effect against gastric duodenal ulcer. And this therapeutic effect is mainly associated with pathways of Ribosome, Alcoholism in gastric tissue, and Amoebiasis, Complement and coagulation cascades in duodenal tissue.