{"title":"Complete viral protein coding genes NS1 and VP1 sequences and phylogenetic analysis of a novel recombinant Mink Enteritis Virus isolated in China","authors":"TIANFEI YU, CHEN SUN, MING LI","doi":"10.56093/ijans.v93i11.133463","DOIUrl":null,"url":null,"abstract":"The primary objective of this study was to detect the presence of MEV in mink samples and investigate the genetic characteristics of the virus. In 2017, a newly identified strain of MEV, named MEV-HLJ, was isolated. The virus’s complete NS1 and VP1 gene sequences were amplified using PCR and subjected to phylogenetic analysis. The results indicated that despite moderate virulence, MEV- HLJ belonged to the attenuated group. Recombination analysis using the GARD programme identified a putative recombination breakpoint at nucleotide position 2,075 between the NS1 and VP1 genes. Separate phylogenetic trees were constructed to confirm the recombinant event. This study provides insights into the genetic diversity and evolution of MEV isolates.","PeriodicalId":13507,"journal":{"name":"Indian Journal of Animal Sciences","volume":null,"pages":null},"PeriodicalIF":0.2000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Animal Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.56093/ijans.v93i11.133463","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0
Abstract
The primary objective of this study was to detect the presence of MEV in mink samples and investigate the genetic characteristics of the virus. In 2017, a newly identified strain of MEV, named MEV-HLJ, was isolated. The virus’s complete NS1 and VP1 gene sequences were amplified using PCR and subjected to phylogenetic analysis. The results indicated that despite moderate virulence, MEV- HLJ belonged to the attenuated group. Recombination analysis using the GARD programme identified a putative recombination breakpoint at nucleotide position 2,075 between the NS1 and VP1 genes. Separate phylogenetic trees were constructed to confirm the recombinant event. This study provides insights into the genetic diversity and evolution of MEV isolates.
期刊介绍：
Articles published in The Indian Journal of Animal Sciences encompass a broad range of research topics in animal health and production related to cattle, buffalo, sheep, goat, camel, equines, pig, rabbit, yak, mithun, poultry and fisheries. Studies involving wildlife species and laboratory animal species that address fundamental questions about their biology will also be considered for publication. All manuscripts must present some new development and must be original, timely, significant and scientifically excellent. Papers will be rejected if standards of care of, or procedures performed on animals are not up to those expected of humane veterinary scientists. At a minimum, standards must meet the International Guiding Principles for Biomedical Research involving Animals, as issued by the Council for International Organizations of Medical Sciences. (C.I.O.M.S., c/o WHO, CH 1211 Geneva 27, Switzerland). Articles reporting new animal disease must follow GOI directive as given in detail in Guidelines to Authors.
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