Measurement of acetylcholinesterase using a two-dimensional electrochemical sensor LAAS

IF 0.5 4区 工程技术 Q4 ENGINEERING, ELECTRICAL & ELECTRONIC Electronics and Communications in Japan Pub Date : 2023-08-31 DOI:10.1002/ecj.12425
Akitsugu Miyairi, Yuki Hasegawa, Hidekazu Uchida
{"title":"Measurement of acetylcholinesterase using a two-dimensional electrochemical sensor LAAS","authors":"Akitsugu Miyairi,&nbsp;Yuki Hasegawa,&nbsp;Hidekazu Uchida","doi":"10.1002/ecj.12425","DOIUrl":null,"url":null,"abstract":"<p>In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples. Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by AChE and releases electrons, making it possible to measure current values in accordance with the amount of substrate using LAAS. When KCl solution was used as the supporting electrolyte and an Ag/AgCl reference electrode as the counter electrode, a correlation between substrate concentration and reaction was confirmed in the range of 1 μM–10 mM. Malathion was then used as an inhibitor of AChE. Malathion phosphorylates and inactivates AChE, which prevents substrate hydrolysis and is expected to decrease the current value. Experimental results showed a decrease in sensor response correlated with inhibitor concentration.</p>","PeriodicalId":50539,"journal":{"name":"Electronics and Communications in Japan","volume":"106 4","pages":""},"PeriodicalIF":0.5000,"publicationDate":"2023-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronics and Communications in Japan","FirstCategoryId":"5","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/ecj.12425","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0

Abstract

In this study, we measured the enzymatic activity of acetylcholinesterase (AChE) using the Light Addressable Amperometric Sensor (LAAS), an electrochemical sensor that can easily measure redox current values of multiple samples. Acetylthiocholine (ATCh) was used as a substrate. ATCh is hydrolyzed by AChE and releases electrons, making it possible to measure current values in accordance with the amount of substrate using LAAS. When KCl solution was used as the supporting electrolyte and an Ag/AgCl reference electrode as the counter electrode, a correlation between substrate concentration and reaction was confirmed in the range of 1 μM–10 mM. Malathion was then used as an inhibitor of AChE. Malathion phosphorylates and inactivates AChE, which prevents substrate hydrolysis and is expected to decrease the current value. Experimental results showed a decrease in sensor response correlated with inhibitor concentration.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
二维电化学传感器LAAS测定乙酰胆碱酯酶
在这项研究中,我们使用光寻址安培传感器(LAAS)测量乙酰胆碱酯酶(AChE)的酶活性,LAAS是一种电化学传感器,可以轻松测量多个样品的氧化还原电流值。以乙酰硫代胆碱(ATCh)为底物。ATCh被AChE水解并释放电子,使得使用LAAS可以根据底物的数量测量电流值。以KCl溶液为载体电解质,Ag/AgCl作为对照电极,底物浓度与反应的相关性在1 μM-10 mM范围内,以马拉硫磷作为AChE抑制剂。马拉硫磷使乙酰胆碱磷酸化并使其失活,从而阻止底物水解,并有望降低电流值。实验结果表明,传感器响应的降低与抑制剂浓度有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Electronics and Communications in Japan
Electronics and Communications in Japan 工程技术-工程:电子与电气
CiteScore
0.60
自引率
0.00%
发文量
45
审稿时长
6-12 weeks
期刊介绍: Electronics and Communications in Japan (ECJ) publishes papers translated from the Transactions of the Institute of Electrical Engineers of Japan 12 times per year as an official journal of the Institute of Electrical Engineers of Japan (IEEJ). ECJ aims to provide world-class researches in highly diverse and sophisticated areas of Electrical and Electronic Engineering as well as in related disciplines with emphasis on electronic circuits, controls and communications. ECJ focuses on the following fields: - Electronic theory and circuits, - Control theory, - Communications, - Cryptography, - Biomedical fields, - Surveillance, - Robotics, - Sensors and actuators, - Micromachines, - Image analysis and signal analysis, - New materials. For works related to the science, technology, and applications of electric power, please refer to the sister journal Electrical Engineering in Japan (EEJ).
期刊最新文献
Issue Information Event-Triggered Robust Control of Robot Manipulators A Hardware Chaotic Neural Network With Gap Junction Models Effects of Controlling Assist Robots to Follow Lumbar Load on Muscle Fatigue Absolute Train Localization Based on the Identification of Surrounding Structures Using 1D LiDAR Sensor
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1