In-vitro effectiveness of a novel herboclean herbal solution in removing pesticides, heavy metals and microbial agents from fruits and vegetables

Shan Sasidharan, Prasannakumari Aiswarya, Janardhanan Hareendran Nair
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Abstract

In recent years, the incidence of undesirable heavy metals, pesticide residues and microbial contaminants in fruits and vegetables has caused severe worldwide public health concerns. In this investigation, we intended to investigate the in-vitro effectiveness of a novel herboclean herbal solution in eliminating pesticides, heavy metals and microbes from the fruits and vegetables collected from various markets. The collected fruits and vegetables were immersed separately in a beaker containing an already prepared herboclean herbal solution for 30 min. The efficacy of the herboclean herbal solution in removing the pesticides and heavy metals was studied using Liquid Chromatography-Mass Spectrometry (LC-MS), and Inductively Plasma Mass Spectrometry (ICPMS), respectively. The reduction in microbes was analyzed by the plate count method. From the result, it was clear that the average removal rate of pesticide residues from fruits and vegetables was 89.89±7.21%. In the case of heavy metals average removal rate was 94.74±9.12%. Whereas in the case of microbes, the removal rate was 90.98±8.38% after washing with Herboclean herbal solution. These results clearly demonstrated that the herboclean herbal solution was very effective in eliminating various pesticides, heavy metals and microbial agents from fresh fruits and vegetables without interfering with their integrity or sensorial parameters. Moreover, this is the first report regarding the efficacy of natural herboclean herbal solutions.
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一种新型herboclean草药溶液去除水果和蔬菜中的农药、重金属和微生物剂的体外效果
近年来,水果和蔬菜中有害重金属、农药残留和微生物污染物的发生率引起了世界范围内严重的公共卫生关注。在这项研究中,我们打算研究一种新型草药溶液在体外去除从不同市场收集的水果和蔬菜中的农药、重金属和微生物的有效性。将收集到的水果和蔬菜分别浸泡在装有已配制好的herboclean草药溶液的烧杯中30 min,分别采用液相色谱-质谱法(LC-MS)和电感等离子体质谱法(ICPMS)研究herboclean草药溶液去除农药和重金属的效果。用平板计数法分析微生物的减少情况。结果表明,果蔬中农药残留的平均去除率为89.89±7.21%。对重金属的平均去除率为94.74±9.12%。而对于微生物,用Herboclean药液洗涤后,去除率为90.98±8.38%。这些结果清楚地表明,herboclean草药溶液在不影响新鲜水果和蔬菜的完整性和感官参数的情况下,可以有效地去除新鲜水果和蔬菜中的各种农药、重金属和微生物剂。此外,这是关于天然草药溶液功效的第一份报告。
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