Evaluation of In-vitro Antioxidant Potential of Bryophillum pinnatum Leaf Juice in Phenyl Hydrazine Treated Goat Erythrocytes

Abstract

Phytochemicals are known to contain a high concentration of therapeutically active principles, which, when consumed in sufficient quantities, can have drug-like effects. Bryophillum pinnatum (Lam.) is a succulent-ethno medicinal xerophyte with a long history of use in traditional Indian medicine. Because reactive oxygen species are the most common etiological factor in many degenerative diseases, controlling them with antioxidants may both treat and prevent disease progression. The current study will look at the ability of fresh leaf juice of Bryophillum pinnatum (Lam.) (FLJBP) to prevent oxidative injury and subsequent hemolysis in goat erythrocytes as a model test system. The catalytic activity of membrane bound enzymes like sodium potassium-ATPase, Ach-Ease, and metabolic enzymes like hexokinase, as well as the NO concentration in goat RBCs, were measured. Phenyl hydrazine was used first, followed by FLJBP at concentrations of 20, 40, 60, 80, and 100g/ml. A dose-dependent (PHZ) increase in Pi liberation by hexokinase and Na+/K+ ATPase, as well as increased formation of thiocholine iodide and enhanced NO scavenging potential, has indicated that flip has good anti-oxidant and anti-haemolytic activity. To record the functional food status of Bryophillum pinnatum (Lam.) species, a more rigorous and stringent battery of pharmacological, phytochemical, and bio analytical studies, followed by observational studies in humans, will be conducted.