Effect of Combined Methanol Extract of Mucuna Poggei and Justicia carnea Leaves on Hematological Indices and Biochemical Indices of Phenylhydrazine Induced Anaemic Albino Rats

Abstract

All parts of Mucuna poggei and Justicia carnea have been known to possess valuable medicinal properties. This calls for the need to determine the effect of combined methanol extract of mucuna poggei and justicia carnea leaves on hematological and biochemical indices of phenylhydrazine induced anaemic albino rats. The study was done using 60 male albino rats. The rats were divided into five groups of five (5) rats each. Group 1 (non-anaemic) was administered water and feed only which served as Normal control. Group 2 was administered Phenylhydrazine only which served as negative control. Group 3 through 5 were Phenylhydrazine induced anaemia treated with combined extract (1:1) at 200 mg/kg, 400 mg/kg and 600 mg/kg respectively. The results showed a significant (p<0.05) increase in Hepatic and renal biomarkers (ALT, AST, ALP, Urea, Creatinine) of the anaemic (negative) control. The combined extract significantly restored the alteration through significant (p<0.05) dose dependent decrease in the assayed hepatic and renal function biomarkers. The hematological indices of the treated rats were maintained compared to the anaemic control. The study showed great potential of combined methanol extract of Mucuna poggei and Justicia carnea leaves capable of ameliorating anaemia.