Fitokimia, Kadar Fenolik Total, dan Flavonoid Total serta Aktivitas Antioksidan Ekstrak n-Heksana Rimpang Temu Hitam (Curcuma aeruginosa Roxb)

Auliya Ilmiawati, Ika Resmeiliana, None Ishika Jauza Nasywa, None Fadia Fahira, None Nafisa Muthia Wafa, None M. Raffi Rayandhika, None Andi Thaariq Muhammad, None Arini Septianti, None Hafizah Fatunisa
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Abstract

The rhizome of black turmeric (Curcuma aeruginosa) is a plant rich in benefits and has been used as a herbal medicine to treat various health problems. This study aims to obtain the n-hexane extract of black turmeric rhizome and perform phytochemical tests, determine total phenolic and total flavonoid levels, as well antioxidant tests using the DPPH method. Extraction of n-hexane from black turmeric rhizome obtained yield of 4.88%, total phenolic content of 3.01 mg QE/g extract and total flavonoids of 6.31 mg QE/g extract. The phytochemical test obtained positive results for the presence of alkaloids and flavonoids; negative tannins, steroids/triterpenoids and saponins. Antioxidant tests using the DPPH method obtained the percentage of DPPH capture ranging from 76-78%, with the largest percentage value obtained when the extract concentration was 125 ppm, which was 78.02%. This shows that black turmeric n-hexane extract has weak antioxidant activity.
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植物化学、总酚含量、总类黄酮和总抗氧化剂活性
黑姜黄(Curcuma aeruginosa)的根茎是一种富含益处的植物,已被用作治疗各种健康问题的草药。本研究旨在获得黑姜黄根茎正己烷提取物,并采用DPPH法进行植物化学试验,测定总酚和总黄酮水平,并进行抗氧化试验。从黑姜黄根茎中提取正己烷,得率为4.88%,总酚含量为3.01 mg QE/g,总黄酮含量为6.31 mg QE/g。植物化学检测结果为生物碱和黄酮类化合物阳性;负单宁,类固醇/三萜和皂苷。采用DPPH法进行抗氧化试验,得到DPPH的捕获率为76 ~ 78%,其中提取液浓度为125 ppm时捕获率最大,为78.02%。说明黑姜黄正己烷提取物具有较弱的抗氧化活性。
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