Visible light photoacoustic ophthalmoscopy and near-infrared-II optical coherence tomography in the mouse eye

IF 5.4 1区物理与天体物理 Q1 OPTICS APL Photonics Pub Date : 2023-10-01 DOI:10.1063/5.0168091

R. Haindl, V. Bellemo, P. Rajendran, B. Tan, M. Liu, B. S. Lee, Q. Zhou, R. A. Leitgeb, W. Drexler, L. Schmetterer, M. Pramanik

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Abstract

Non-invasive imaging plays a crucial role in diagnosing and studying eye diseases. However, existing photoacoustic ophthalmoscopy (PAOM) techniques in mice have limitations due to handling restrictions, suboptimal optical properties, limited availability of light sources, and permissible light fluence at the retina. This study introduces an innovative approach that utilizes Rose Bengal, a contrast agent, to enhance PAOM contrast. This enables visualization of deeper structures, such as the choroidal vasculature and sclera in the mouse eye, using visible light. The integration of near-infrared-II (NIR-II) optical coherence tomography provides additional tissue contrast and insights into potential NIR-II PAOM capabilities. To optimize imaging, we developed a cost-effective 3D printable mouse eye phantom and a fully 3D printable tip/tilt mouse platform. This solution elevates PAOM to a user-friendly technology, which can be used to address pressing research questions concerning several ocular diseases, such as myopia, glaucoma, and/or age-related macular degeneration in the future.

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小鼠眼的可见光光声检及近红外光学相干断层扫描

无创成像在眼科疾病的诊断和研究中起着至关重要的作用。然而，现有的小鼠光声检眼镜(PAOM)技术由于操作限制、光学性能不理想、光源可用性有限以及视网膜允许的光影响而存在局限性。本研究介绍了一种利用玫瑰孟加拉造影剂来增强PAOM造影剂的创新方法。这使得使用可见光可以可视化更深层的结构，如小鼠眼睛中的脉络膜血管和巩膜。近红外ii (NIR-II)光学相干断层扫描的集成提供了额外的组织对比，并深入了解了NIR-II潜在的PAOM能力。为了优化成像，我们开发了一个具有成本效益的3D打印鼠标眼幻影和一个完全3D打印的倾斜/倾斜鼠标平台。该解决方案将PAOM提升为一种用户友好的技术，可用于解决未来几种眼部疾病的紧迫研究问题，如近视、青光眼和/或年龄相关性黄斑变性。

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来源期刊

APL Photonics Physics and Astronomy-Atomic and Molecular Physics, and Optics

CiteScore

10.30

自引率

3.60%

发文量

107

审稿时长

19 weeks

期刊介绍： APL Photonics is the new dedicated home for open access multidisciplinary research from and for the photonics community. The journal publishes fundamental and applied results that significantly advance the knowledge in photonics across physics, chemistry, biology and materials science.