Comparison of smear layer removing efficacy of Cold Atmospheric Pressure (CAP) Plasma Jet with different chelating agents. An ex-vivo study

Ankita Kapoor, Pravin Kumar, Ram Prakash, Kirti Chaudhry, Rajat Sharma, Arun Kumar Duraisamy
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Abstract

The present study aimed to assess the effectiveness of different final irrigation regimens (Cold Atmospheric Pressure Plasma Jet, MTAD, and EDTA) in removing the smear layer from intra-radicular dentin using a Scanning Electron Microscope (SEM). Eighty-four mandibular premolars were prepared with ProTaper Universal hand files and were equally divided into four groups i.e. Normal saline (control), EDTA, MTAD and CAP Plasma Jet. Prepared samples in the control, EDTA and MTAD groups were irrigated with 5 milliliters of the irrigant, and it was retained for 2 min. In the CAP Plasma Jet group, the plasma plume was directed towards the canal lumen for 2 min. The smear layer removal of all the groups was evaluated at the coronal, middle and apical thirds. Statistical analysis was performed using Kruskal–Wallis test followed by Dunn’s test. Evaluation by SEM showed that the smear layer removal ability of MTAD and EDTA were significantly better than CAP Plasma Jet (p < 0.05). While CAP Plasma Jet showed results comparable to EDTA in the coronal third. In the middle and apical third of the canal, its effect was comparable to the control group (p > 0.05). MTAD and EDTA aided in better smear layer removal than the CAP Plasma Jet in the coronal, middle, and apical third of the test samples. CAP Plasma jet performed better in the coronal third.
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不同螯合剂对冷常压等离子体射流除污效果的影响。离体研究
本研究旨在通过扫描电子显微镜(SEM)评估不同最终冲洗方案(冷大气压等离子体喷射、MTAD和EDTA)去除根状牙本质内涂抹层的有效性。采用ProTaper通用手锉制备84颗下颌前磨牙,随机分为生理盐水组(对照组)、EDTA组、MTAD组和CAP等离子体喷射组。对照组、EDTA组和MTAD组制备的样品用5毫升冲洗液冲洗,并保留2分钟。CAP等离子体喷射组将等离子体羽流指向管腔2分钟。评估各组在冠状、中端和根尖三分之一处的涂片层去除情况。采用Kruskal-Wallis检验和Dunn检验进行统计分析。SEM评价表明,MTAD和EDTA的去除涂抹层能力显著优于CAP等离子体射流(p < 0.05)。而CAP等离子体喷射在冠状三分之一的结果与EDTA相当。在根管中部和根尖三分之一处,其效果与对照组相当(p > 0.05)。与CAP等离子体射流相比,MTAD和EDTA在冠状、中间和根尖三分之一的测试样品中有助于更好地去除涂抹层。等离子体射流在冠状区表现较好。
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