Pub Date : 2024-10-29eCollection Date: 2024-01-01DOI: 10.2340/biid.v11.42172
Andreas Koutroulis, Vasileios Kapralos, Dag Ørstavik, Pia Titterud Sunde
The placement of root filling materials aims to prevent the occurrence of post-treatment apical periodontitis following completion of endodontic treatment. Materials should possess properties that will not permit bacterial invasion and infection, namely excellent sealing ability and/or antibacterial properties. In root-end filling procedures or repair of root perforations, the root filling materials are placed in a particularly challenging clinical environment, as they interface with a relatively large area with the periradicular tissues. The biological properties of these materials are therefore of significant importance. The current review discusses the most widely used materials for endodontic surgery (i.e., root-end filling and perforation repair), with particular focus on their biological characteristics, namely antibacterial properties and interactions with host tissue cells, together with clinical studies. Properties of amalgam, glass ionomer cements (GICs), resin systems, zinc oxide eugenol-based cements and hydraulic calcium silicate cements (HCSCs), together with representative and well-researched commercial materials in the context of their use in endodontic surgery are presented. While the use of HCSCs seems to offer several biological advantages, together with addressing issues with the initial formulation in the most recent versions, materials with different chemical compositions, such as zinc oxide eugenol-based cements, are still in use and appear to provide similar clinical success rates to HCSCs. Thus, the significance of the currently available materials on clinical outcomes remains unclear.
{"title":"Root-filling materials for endodontic surgery: biological and clinical aspects.","authors":"Andreas Koutroulis, Vasileios Kapralos, Dag Ørstavik, Pia Titterud Sunde","doi":"10.2340/biid.v11.42172","DOIUrl":"10.2340/biid.v11.42172","url":null,"abstract":"<p><p>The placement of root filling materials aims to prevent the occurrence of post-treatment apical periodontitis following completion of endodontic treatment. Materials should possess properties that will not permit bacterial invasion and infection, namely excellent sealing ability and/or antibacterial properties. In root-end filling procedures or repair of root perforations, the root filling materials are placed in a particularly challenging clinical environment, as they interface with a relatively large area with the periradicular tissues. The biological properties of these materials are therefore of significant importance. The current review discusses the most widely used materials for endodontic surgery (i.e., root-end filling and perforation repair), with particular focus on their biological characteristics, namely antibacterial properties and interactions with host tissue cells, together with clinical studies. Properties of amalgam, glass ionomer cements (GICs), resin systems, zinc oxide eugenol-based cements and hydraulic calcium silicate cements (HCSCs), together with representative and well-researched commercial materials in the context of their use in endodontic surgery are presented. While the use of HCSCs seems to offer several biological advantages, together with addressing issues with the initial formulation in the most recent versions, materials with different chemical compositions, such as zinc oxide eugenol-based cements, are still in use and appear to provide similar clinical success rates to HCSCs. Thus, the significance of the currently available materials on clinical outcomes remains unclear.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"11 ","pages":"42172"},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11653206/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142857193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-10-01eCollection Date: 2024-01-01DOI: 10.2340/biid.v11.41933
Sigfus Thor Eliasson, Jon Einar Dahl
Objectives: To investigate whether acid etch contamination of silane-treated composite influenced repair bond strength and whether silane contamination on dentin influenced composite bond strength to dentin.
Materials and methods: Forty composite blocks stored in water for 4 weeks were divided into four groups. Specimens in groups 1-3 were coated with Bis-Silane and contaminated with acid etch + water spray (group 1) or water spray (group 2). Group 3 was not contaminated. Group 4 was untreated. The occlusal third of 60 third molars was cut off, ground flat, and divided into three groups. After etching, the surfaces in groups A and B were contaminated with Bis-silane. The contaminated surfaces in group A were re-etched.Each composite repair group and composite-dentin group was divided into two subgroups receiving Adper Scotchbond 1 XT or Clearfil SE Bond 2 adhesives followed by a composite build up. After ageing for 3 months, specimens were sectioned into 1.1 mm × 1.1 mm rods for tensile testing and strength calculated at fracture. The fracture was examined using microscope.
Results: Bis-Silane surface treatment increased the repair bond strength. Contamination with acid reduced the strength of the repair bond. Similar results were obtained for both adhesives. Tooth surface contamination with silane reduced the bond strength between dentin and composite. Additional acid etching or water spray on silane contaminated dentin did not influence the weakened bond strength. Most fractures were adhesive type.
Conclusions: Silane contamination on etched dentin and acid etch contamination on silanized composite surfaces significantly reduced tensile bond strength.
{"title":"Silane and acid etch cross contamination of dentin and composite reduced µ-tensile bond strength.","authors":"Sigfus Thor Eliasson, Jon Einar Dahl","doi":"10.2340/biid.v11.41933","DOIUrl":"https://doi.org/10.2340/biid.v11.41933","url":null,"abstract":"<p><strong>Objectives: </strong>To investigate whether acid etch contamination of silane-treated composite influenced repair bond strength and whether silane contamination on dentin influenced composite bond strength to dentin.</p><p><strong>Materials and methods: </strong>Forty composite blocks stored in water for 4 weeks were divided into four groups. Specimens in groups 1-3 were coated with Bis-Silane and contaminated with acid etch + water spray (group 1) or water spray (group 2). Group 3 was not contaminated. Group 4 was untreated. The occlusal third of 60 third molars was cut off, ground flat, and divided into three groups. After etching, the surfaces in groups A and B were contaminated with Bis-silane. The contaminated surfaces in group A were re-etched.Each composite repair group and composite-dentin group was divided into two subgroups receiving Adper Scotchbond 1 XT or Clearfil SE Bond 2 adhesives followed by a composite build up. After ageing for 3 months, specimens were sectioned into 1.1 mm × 1.1 mm rods for tensile testing and strength calculated at fracture. The fracture was examined using microscope.</p><p><strong>Results: </strong>Bis-Silane surface treatment increased the repair bond strength. Contamination with acid reduced the strength of the repair bond. Similar results were obtained for both adhesives. Tooth surface contamination with silane reduced the bond strength between dentin and composite. Additional acid etching or water spray on silane contaminated dentin did not influence the weakened bond strength. Most fractures were adhesive type.</p><p><strong>Conclusions: </strong>Silane contamination on etched dentin and acid etch contamination on silanized composite surfaces significantly reduced tensile bond strength.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"11 ","pages":"41933"},"PeriodicalIF":0.0,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11457355/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142395683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-22eCollection Date: 2024-01-01DOI: 10.2340/biid.v11.41161
Kristina Johansson, Liselotte Paulsson, Helena Christell
Objectives: To assess inter- and intrarater reliability and agreement for measurements of root lengths using multiplanar reconstruction (MPR) in cone beam computed tomography (CBCT) examinations.Furthermore, to determine whether using MPR from different CBCT machines was a reliable and reproducible method for assessment of root length during orthodontic treatment of adolescents.
Materials and methods: A total of 40 CBCT examinations obtained before, during and after orthodontic treatment of 14 adolescents, with fixed appliances from a multicentre randomised controlled trial, were used. All roots from the incisors to the first molars were measured by two independent raters and in accordance with a protocol preceded by a multi-step calibration. Reliability was assessed by intra class correlation (ICC). Agreement was assessed by measurement error according to the Dahlberg formula and Bland-Altman plot.
Results: The number of repeated measurements varied from 436 to 474 for the different timepoints. Good to excellent inter- and intrarater reliability for different tooth groups and timepoints were shown. Measurement error for inter- and intrarater agreement varied between 0.41 mm and 0.77 mm. The Bland-Altman plot with 95% limits of agreement varied between +1.43 mm and -2.01 mm for different tooth groups and timepoints.
Conclusions: The results of this study indicate that CBCT using MPR from different machines is a reproducible method for measuring root length during different phases of orthodontic treatment. When interpreting root shortening measurements in CBCT using MPR for clinical or research purposes, values below 2 mm should be approached with caution, as they may contain measurement errors.
{"title":"Reliability and agreement of root length measurements during orthodontic treatment in images from different CBCT machines using multiplanar reconstruction.","authors":"Kristina Johansson, Liselotte Paulsson, Helena Christell","doi":"10.2340/biid.v11.41161","DOIUrl":"10.2340/biid.v11.41161","url":null,"abstract":"<p><strong>Objectives: </strong>To assess inter- and intrarater reliability and agreement for measurements of root lengths using multiplanar reconstruction (MPR) in cone beam computed tomography (CBCT) examinations.Furthermore, to determine whether using MPR from different CBCT machines was a reliable and reproducible method for assessment of root length during orthodontic treatment of adolescents.</p><p><strong>Materials and methods: </strong>A total of 40 CBCT examinations obtained before, during and after orthodontic treatment of 14 adolescents, with fixed appliances from a multicentre randomised controlled trial, were used. All roots from the incisors to the first molars were measured by two independent raters and in accordance with a protocol preceded by a multi-step calibration. Reliability was assessed by intra class correlation (ICC). Agreement was assessed by measurement error according to the Dahlberg formula and Bland-Altman plot.</p><p><strong>Results: </strong>The number of repeated measurements varied from 436 to 474 for the different timepoints. Good to excellent inter- and intrarater reliability for different tooth groups and timepoints were shown. Measurement error for inter- and intrarater agreement varied between 0.41 mm and 0.77 mm. The Bland-Altman plot with 95% limits of agreement varied between +1.43 mm and -2.01 mm for different tooth groups and timepoints.</p><p><strong>Conclusions: </strong>The results of this study indicate that CBCT using MPR from different machines is a reproducible method for measuring root length during different phases of orthodontic treatment. When interpreting root shortening measurements in CBCT using MPR for clinical or research purposes, values below 2 mm should be approached with caution, as they may contain measurement errors.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"11 ","pages":"41161"},"PeriodicalIF":0.0,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142127525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-22eCollection Date: 2024-01-01DOI: 10.2340/biid.v11.41367
Anne Peutzfeldt, Jon E Dahl
{"title":"Recipient of Biomaterial Investigations in Dentistry's Young Author Award 2023.","authors":"Anne Peutzfeldt, Jon E Dahl","doi":"10.2340/biid.v11.41367","DOIUrl":"https://doi.org/10.2340/biid.v11.41367","url":null,"abstract":"","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"11 ","pages":"41367"},"PeriodicalIF":0.0,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11359182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142115539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective: This study evaluated the sealing ability of different biomaterials as intra-orifice barriers in the internal bleaching of discolored teeth with the walking bleaching technique. The release of hydroxyl ions from the bleaching materials can cause cervical root resorption, making it necessary to use intra-orifice barrier materials to prevent this issue.
Materials and methods: In the current study, the high-performance liquid chromatography (HPLC) method was used to measure the released hydroxyl ions. The study included 90 single-rooted and single-canal premolars, which were divided into four groups based on the intra-orifice barrier materials used (mineral trioxide aggregate [MTA], calcium-enriched mixture [CEM], Biodentine, and MTA+PG) and the type of bleaching material (sodium perborate + water or sodium perborate + hydrogen peroxide 30%). Two control groups were also considered in this study: a positive control group, where sodium perborate and hydrogen peroxide were placed inside the pulp chamber without any intra-orifice barriers; and a negative control group, where no bleaching agent or surgical obstruction was used, and the root surface was covered with wax up to the cemento-enamel junction (CEJ) level.
Results: The results showed that there was a significant difference in the concentration of hydroxyl ions released among the studied groups. The amount of hydroxyl ion released was highest in the positive control group and lowest in the CEM group. Among the intra-orifice barrier materials used, CEM cement was found to be the most appropriate material for use in the step-by-step internal bleaching method.
Conclusions: The study highlights the importance of using appropriate intra-orifice barrier materials to prevent root cervical resorption in internal bleaching procedures.
{"title":"The sealing ability of different endodontic biomaterials as an intra-orifice barrier: evaluation with high-performance liquid chromatography.","authors":"Sahar Shakouei, Negin Ghasemi, Parvin Zakeri-Milani, Afsaneh Shahali, Mahdieh Alipour","doi":"10.2340/biid.v11.41069","DOIUrl":"10.2340/biid.v11.41069","url":null,"abstract":"<p><strong>Objective: </strong>This study evaluated the sealing ability of different biomaterials as intra-orifice barriers in the internal bleaching of discolored teeth with the walking bleaching technique. The release of hydroxyl ions from the bleaching materials can cause cervical root resorption, making it necessary to use intra-orifice barrier materials to prevent this issue.</p><p><strong>Materials and methods: </strong>In the current study, the high-performance liquid chromatography (HPLC) method was used to measure the released hydroxyl ions. The study included 90 single-rooted and single-canal premolars, which were divided into four groups based on the intra-orifice barrier materials used (mineral trioxide aggregate [MTA], calcium-enriched mixture [CEM], Biodentine, and MTA+PG) and the type of bleaching material (sodium perborate + water or sodium perborate + hydrogen peroxide 30%). Two control groups were also considered in this study: a positive control group, where sodium perborate and hydrogen peroxide were placed inside the pulp chamber without any intra-orifice barriers; and a negative control group, where no bleaching agent or surgical obstruction was used, and the root surface was covered with wax up to the cemento-enamel junction (CEJ) level.</p><p><strong>Results: </strong>The results showed that there was a significant difference in the concentration of hydroxyl ions released among the studied groups. The amount of hydroxyl ion released was highest in the positive control group and lowest in the CEM group. Among the intra-orifice barrier materials used, CEM cement was found to be the most appropriate material for use in the step-by-step internal bleaching method.</p><p><strong>Conclusions: </strong>The study highlights the importance of using appropriate intra-orifice barrier materials to prevent root cervical resorption in internal bleaching procedures.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"11 ","pages":"41069"},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
L. Marger, M. Abdelaziz, E. Bella, I. Krejci, C. I. Anton Y Otero
Objectives: The study aims to investigate the effects of laser-activated irrigation on infiltration and microleakage of a dual-curing resin cement applied as a root canal sealer. Methods: Thirty-eight extracted upper molars were attributed to four experimental groups. Roots were mechanically enlarged and disinfected (NaOCl). Control samples (n = 11) were irrigated with conventional needles and three different lasers were used to activate the irrigant in the other groups (n = 9): 2.94 µm Er:YAG laser, 9,300 µm CO2 laser and 808 nm diode laser with a modified black coated laser tip. Final irrigation was performed in each group with ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl) and sodium chloride (NaCl) activated with lasers and in the control without activation.Dentin tubules were then labeled with a red fluorophore (Rhodamine B) and the root canals were sealed with a dual-curing resin cement (Paracore). The cement fixed the dye on the sealed and infiltrated dentin parts. To remove the Rhodamine not fixed by the cement, roots were then sectioned horizontally and immersed in hydrogen peroxide (H2O2). The empty dentin tubules spaces were then labeled with a green fluorophore (Fluorescein) allowing the visualization of infiltration and microleakage by confocal microscopy. Results: Percentages of infiltration were significantly higher in the middle root third of the control and Er:YAG laser-activated samples compared to CO2 or diode laser groups. Microleakage was present in all experimental groups but significantly less after CO2 laser activation. Conclusion: Laser-activated irrigation impacted resin cement infiltration and microleakage dependent on the applied wavelength. Er:YAG laser activation led to higher values of infiltration and microleakage compared to CO2 and diode lasers.
研究目的本研究旨在探讨激光激活灌洗对用作根管封闭剂的双固化树脂水泥的渗透和微渗漏的影响:方法:38 颗拔出的上磨牙分为四个实验组。对牙根进行机械扩大和消毒(NaOCl)。对照组样本(n = 11)使用传统针头灌洗,其他组样本(n = 9)使用三种不同的激光激活灌洗剂:2.94 µm Er:YAG 激光器、9,300 µm CO2 激光器和 808 nm 带有改良黑色涂层激光器尖端的二极管激光器。然后用红色荧光团(罗丹明 B)标记牙本质小管,并用双固化树脂水门汀(Paracore)密封根管。然后用红色荧光团(罗丹明 B)标记牙本质小管,再用双固化树脂水门汀(Paracore)密封根管。为了去除未被粘接剂固定的罗丹明,将牙根水平切片并浸泡在过氧化氢(H2O2)中。然后用绿色荧光团(荧光素)标记空的牙本质小管空间,这样就能通过共聚焦显微镜观察到浸润和微渗漏:结果:与 CO2 或二极管激光组相比,对照组和 Er:YAG 激光激活组样品中三分之一根部的浸润比例明显更高。所有实验组都存在微渗漏,但 CO2 激光激活后微渗漏明显减少:结论:激光激活灌注对树脂粘结剂渗透和微渗漏的影响取决于所使用的波长。与 CO2 和二极管激光相比,Er:YAG 激光激活导致的浸润和微渗漏值更高。
{"title":"An in vitro study on the influence of laser-activated irrigation on infiltration and leakage of a dual curing-resin cement as an endodontic sealer","authors":"L. Marger, M. Abdelaziz, E. Bella, I. Krejci, C. I. Anton Y Otero","doi":"10.2340/biid.v11.41098","DOIUrl":"https://doi.org/10.2340/biid.v11.41098","url":null,"abstract":"Objectives: The study aims to investigate the effects of laser-activated irrigation on infiltration and microleakage of a dual-curing resin cement applied as a root canal sealer.\u0000Methods: Thirty-eight extracted upper molars were attributed to four experimental groups. Roots were mechanically enlarged and disinfected (NaOCl). Control samples (n = 11) were irrigated with conventional needles and three different lasers were used to activate the irrigant in the other groups (n = 9): 2.94 µm Er:YAG laser, 9,300 µm CO2 laser and 808 nm diode laser with a modified black coated laser tip. Final irrigation was performed in each group with ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl) and sodium chloride (NaCl) activated with lasers and in the control without activation.Dentin tubules were then labeled with a red fluorophore (Rhodamine B) and the root canals were sealed with a dual-curing resin cement (Paracore). The cement fixed the dye on the sealed and infiltrated dentin parts. To remove the Rhodamine not fixed by the cement, roots were then sectioned horizontally and immersed in hydrogen peroxide (H2O2). The empty dentin tubules spaces were then labeled with a green fluorophore (Fluorescein) allowing the visualization of infiltration and microleakage by confocal microscopy.\u0000Results: Percentages of infiltration were significantly higher in the middle root third of the control and Er:YAG laser-activated samples compared to CO2 or diode laser groups. Microleakage was present in all experimental groups but significantly less after CO2 laser activation.\u0000Conclusion: Laser-activated irrigation impacted resin cement infiltration and microleakage dependent on the applied wavelength. Er:YAG laser activation led to higher values of infiltration and microleakage compared to CO2 and diode lasers.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"67 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141653150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anne Breivik, Aida Mulic, A. Sehic, H. Valen, S. Kopperud, Linda Stein, Qalbi Khan
Objective: This study aimed to quantitatively investigate the accumulation of Streptococcus mutans biofilm on enamel and root surfaces and assess the amount of biofilm removal using (1) experimental toothpaste and (2) water, in a closed system of flow chamber. Methods: Eight sound premolars were embedded in epoxy resin and polished with silicon carbide grinding papers to display enamel and root surfaces. To mimic biofilm, cultures of Streptococcus mutans were prepared and grown on the tooth surfaces over night before they were exposed to either 2 liters of Milli Q water or 2 liters of 40% experimental toothpaste in the flow chamber. The amount of biofilm was measured and quantified in Fluorescence microscopy. Mean fluorescence values were recorded and analysed using Microsoft® Excel® (MS Excel 2016). Results: The ability to grow biofilm was equally present at both the enamel and root surfaces. The use of water and 40% experimental toothpaste showed a significant reduction of areas covered with biofilm on both enamel and root dentin in comparison to untreated surfaces (p < 0.01). Significantly more biofilm was removed from enamel compared to root surfaces when treated with either water and toothpaste (p < 0.01). Slightly less biofilm was removed by the use of water compared to toothpaste on both enamel and root dentin surfaces, although the differences were not statistically significant. Conclusion: The results indicate that less biofilm is removed from the root surfaces than enamel by the use of water and 40% experimental toothpaste in flow chamber. Assessing oral biofilm accumulation and monitoring biofilm formation on enamel and root dentin surfaces give oral health professionals important directions that could strenghten the significance of dental caries prevention. Improving older individuals’ oral hygiene practices should therefore be considered an important measure to prevent root caries.
{"title":"Accumulation and removal of Streptococcus mutans biofilm on enamel and root surfaces in vitro","authors":"Anne Breivik, Aida Mulic, A. Sehic, H. Valen, S. Kopperud, Linda Stein, Qalbi Khan","doi":"10.2340/biid.v11.41059","DOIUrl":"https://doi.org/10.2340/biid.v11.41059","url":null,"abstract":"Objective: This study aimed to quantitatively investigate the accumulation of Streptococcus mutans biofilm on enamel and root surfaces and assess the amount of biofilm removal using (1) experimental toothpaste and (2) water, in a closed system of flow chamber.\u0000Methods: Eight sound premolars were embedded in epoxy resin and polished with silicon carbide grinding papers to display enamel and root surfaces. To mimic biofilm, cultures of Streptococcus mutans were prepared and grown on the tooth surfaces over night before they were exposed to either 2 liters of Milli Q water or 2 liters of 40% experimental toothpaste in the flow chamber. The amount of biofilm was measured and quantified in Fluorescence microscopy. Mean fluorescence values were recorded and analysed using Microsoft® Excel® (MS Excel 2016).\u0000Results: The ability to grow biofilm was equally present at both the enamel and root surfaces. The use of water and 40% experimental toothpaste showed a significant reduction of areas covered with biofilm on both enamel and root dentin in comparison to untreated surfaces (p < 0.01). Significantly more biofilm was removed from enamel compared to root surfaces when treated with either water and toothpaste (p < 0.01). Slightly less biofilm was removed by the use of water compared to toothpaste on both enamel and root dentin surfaces, although the differences were not statistically significant.\u0000Conclusion: The results indicate that less biofilm is removed from the root surfaces than enamel by the use of water and 40% experimental toothpaste in flow chamber. Assessing oral biofilm accumulation and monitoring biofilm formation on enamel and root dentin surfaces give oral health professionals important directions that could strenghten the significance of dental caries prevention. Improving older individuals’ oral hygiene practices should therefore be considered an important measure to prevent root caries.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141654108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Purpose: The purpose of this study was to investigate the correlation between the size and concentration of titanium particles and the osteogenic response of human osteoblasts (HOB). Materials and Methods: Different concentrations of titanium dioxide nano- and micro-particles were prepared and their biocompatibility on HOBs was analyzed using XTT assay. The changes in the actin cytoskeletal organization were studied by confocal laser scanning microscopy. The generation of intracellular reactive oxygen species (ROS) by HOBs after exposure to titanium dioxide particles was analyzed using ROS assay. Besides, the osteogenic potential represented by alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels, and biomineralization were analyzed. Results: Short-term interaction of titanium dioxide nano- and micro-particles did not induce toxicity to HOBs. However, cells treated with 100 μg/mL titanium dioxide nano- and micro-particles demonstrated higher ROS generation compared to control. Besides, cells treated with 100 µg/mL titanium dioxide nanoparticles showed higher alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels and biomineralization compared to titanium dioxide microparticles. Conclusion: Collectively, the study found titanium dioxide nanoparticles to be more biocompatible than microparticles providing an insight into the capability of nanostructures in supporting osteoblast differentiation and its plausibility in biomedical applications.
{"title":"Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study","authors":"S. Sheela, Waad Kheder, Rani Samsudin","doi":"10.2340/biid.v11.40843","DOIUrl":"https://doi.org/10.2340/biid.v11.40843","url":null,"abstract":"Purpose: The purpose of this study was to investigate the correlation between the size and concentration of titanium particles and the osteogenic response of human osteoblasts (HOB). \u0000Materials and Methods: Different concentrations of titanium dioxide nano- and micro-particles were prepared and their biocompatibility on HOBs was analyzed using XTT assay. The changes in the actin cytoskeletal organization were studied by confocal laser scanning microscopy. The generation of intracellular reactive oxygen species (ROS) by HOBs after exposure to titanium dioxide particles was analyzed using ROS assay. Besides, the osteogenic potential represented by alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels, and biomineralization were analyzed.\u0000Results: Short-term interaction of titanium dioxide nano- and micro-particles did not induce toxicity to HOBs. However, cells treated with 100 μg/mL titanium dioxide nano- and micro-particles demonstrated higher ROS generation compared to control. Besides, cells treated with 100 µg/mL titanium dioxide nanoparticles showed higher alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels and biomineralization compared to titanium dioxide microparticles. \u0000Conclusion: Collectively, the study found titanium dioxide nanoparticles to be more biocompatible than microparticles providing an insight into the capability of nanostructures in supporting osteoblast differentiation and its plausibility in biomedical applications.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141349645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Endodontic sealers and cements used in root canal treatment have different compositions and properties. Common to all materials is that their primary goal is to fill gaps and voids, making a permanent seal of the root canal system. Furthermore, aspects such as antibacterial properties, cytotoxicity, setting time, solubility and biocompatibility are also crucial and ought to be considered. Over the years, a shift in the view on the importance of these aspects has ocurred. Whereas the antibacterial properties were considered important when the technical factors in endodontics were less developed, the sealing ability and biocompatibility have later been considered the most critical factors. The introduction of tricalcium silicate cements and sealers has led to a renewed interest in material properties, as these cements seem to have good sealing ability and at the same time combine favourable antimicrobial effects with excellent biocompatibility. This review discusses how the various properties of root canal sealers and cements may conflict with the primary aim of providing a permanent seal of the root canal system.
{"title":"Biological properties versus solubility of endodontic sealers and cements","authors":"Inge Fristad, Sivakami Haug, A. Bårdsen","doi":"10.2340/biid.v11.40863","DOIUrl":"https://doi.org/10.2340/biid.v11.40863","url":null,"abstract":"Endodontic sealers and cements used in root canal treatment have different compositions and properties. Common to all materials is that their primary goal is to fill gaps and voids, making a permanent seal of the root canal system. Furthermore, aspects such as antibacterial properties, cytotoxicity, setting time, solubility and biocompatibility are also crucial and ought to be considered. Over the years, a shift in the view on the importance of these aspects has ocurred. Whereas the antibacterial properties were considered important when the technical factors in endodontics were less developed, the sealing ability and biocompatibility have later been considered the most critical factors. The introduction of tricalcium silicate cements and sealers has led to a renewed interest in material properties, as these cements seem to have good sealing ability and at the same time combine favourable antimicrobial effects with excellent biocompatibility. This review discusses how the various properties of root canal sealers and cements may conflict with the primary aim of providing a permanent seal of the root canal system. ","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"7 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141267392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Elizabeth Madla-Cruz, Vanascheck Dasaev Villanueva-Pérez, Myriam A. De la Garza-Ramos, J. Flores-Treviño, Idalia Rodríguez-Delgado, Fanny López-Martinez
ObjectiveThis study aimed to compare the antimicrobial effect of three endodontic sealers (AH Plus, Mineral trioxide aggregate [MTA] Fillapex, and BioRoot RCS) with and without amoxicillin against E. faecalis. MethodologyAmoxicillin, equivalent to 10% of the sealers’ total weight, was mixed with the sealers. Another batch was prepared without amoxicillin. The direct contact test (DCT) and the agar diffusion test were used to assess the antibacterial effect. Results were analysed using one-way analysis of variance (ANOVA), the F-test, and the Kruskal-Wallis test. ResultsAH Plus significantly suppressed E. faecalis without the addition of amoxicillin in the DCT (p = 0.011), while in the agar diffusion test, BioRoot RCS had a larger inhibition zone than the control (p < 0.001). When amoxicillin was added to the sealers, AH Plus (p = 0.003) and MTA Fillapex (p = 0.042) reduced E. faecalis growth. In contrast, all three sealers showed larger inhibition zones than the control (p = 0.001), with AH Plus displaying a larger inhibition zone than MTA Fillapex (p = 0.042) and BioRoot RCS (p = 0.032). ConclusionsIt was thus concluded that the addition of amoxicillin to endodontic sealers enhances their antimicrobial activity against E. faecalis.
{"title":"An in vitro evaluation of endodontic sealers and an antibiotic to assess their antimicrobial effect against Enterococcus faecalis","authors":"Elizabeth Madla-Cruz, Vanascheck Dasaev Villanueva-Pérez, Myriam A. De la Garza-Ramos, J. Flores-Treviño, Idalia Rodríguez-Delgado, Fanny López-Martinez","doi":"10.2340/biid.v11.40646","DOIUrl":"https://doi.org/10.2340/biid.v11.40646","url":null,"abstract":"ObjectiveThis study aimed to compare the antimicrobial effect of three endodontic sealers (AH Plus, Mineral trioxide aggregate [MTA] Fillapex, and BioRoot RCS) with and without amoxicillin against E. faecalis. \u0000MethodologyAmoxicillin, equivalent to 10% of the sealers’ total weight, was mixed with the sealers. Another batch was prepared without amoxicillin. The direct contact test (DCT) and the agar diffusion test were used to assess the antibacterial effect. Results were analysed using one-way analysis of variance (ANOVA), the F-test, and the Kruskal-Wallis test. \u0000ResultsAH Plus significantly suppressed E. faecalis without the addition of amoxicillin in the DCT (p = 0.011), while in the agar diffusion test, BioRoot RCS had a larger inhibition zone than the control (p < 0.001). When amoxicillin was added to the sealers, AH Plus (p = 0.003) and MTA Fillapex (p = 0.042) reduced E. faecalis growth. In contrast, all three sealers showed larger inhibition zones than the control (p = 0.001), with AH Plus displaying a larger inhibition zone than MTA Fillapex (p = 0.042) and BioRoot RCS (p = 0.032). \u0000ConclusionsIt was thus concluded that the addition of amoxicillin to endodontic sealers enhances their antimicrobial activity against E. faecalis.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"6 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141099550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}