Biomaterial investigations in dentistry最新文献

Reliability and agreement of root length measurements during orthodontic treatment in images from different CBCT machines using multiplanar reconstruction. 使用多平面重建技术，在不同 CBCT 设备的图像中测量正畸治疗期间牙根长度的可靠性和一致性。

Biomaterial investigations in dentistry

Pub Date : 2024-08-22 eCollection Date: 2024-01-01 DOI: 10.2340/biid.v11.41161

Kristina Johansson, Liselotte Paulsson, Helena Christell

Objectives: To assess inter- and intrarater reliability and agreement for measurements of root lengths using multiplanar reconstruction (MPR) in cone beam computed tomography (CBCT) examinations.Furthermore, to determine whether using MPR from different CBCT machines was a reliable and reproducible method for assessment of root length during orthodontic treatment of adolescents.

Materials and methods: A total of 40 CBCT examinations obtained before, during and after orthodontic treatment of 14 adolescents, with fixed appliances from a multicentre randomised controlled trial, were used. All roots from the incisors to the first molars were measured by two independent raters and in accordance with a protocol preceded by a multi-step calibration. Reliability was assessed by intra class correlation (ICC). Agreement was assessed by measurement error according to the Dahlberg formula and Bland-Altman plot.

Results: The number of repeated measurements varied from 436 to 474 for the different timepoints. Good to excellent inter- and intrarater reliability for different tooth groups and timepoints were shown. Measurement error for inter- and intrarater agreement varied between 0.41 mm and 0.77 mm. The Bland-Altman plot with 95% limits of agreement varied between +1.43 mm and -2.01 mm for different tooth groups and timepoints.

Conclusions: The results of this study indicate that CBCT using MPR from different machines is a reproducible method for measuring root length during different phases of orthodontic treatment. When interpreting root shortening measurements in CBCT using MPR for clinical or research purposes, values below 2 mm should be approached with caution, as they may contain measurement errors.

目的评估在锥形束计算机断层扫描（CBCT）检查中使用多平面重建（MPR）测量牙根长度的内部和外部可靠性和一致性。此外，确定在青少年正畸治疗过程中使用不同CBCT机器的MPR评估牙根长度是否是一种可靠且可重复的方法：研究使用了多中心随机对照试验中为 14 名青少年正畸治疗前、正畸治疗中和正畸治疗后进行的共 40 次 CBCT 检查。从门牙到第一磨牙的所有牙根均由两名独立的评定者按照多步骤校准前的协议进行测量。可靠性通过类内相关性（ICC）进行评估。根据 Dahlberg 公式和 Bland-Altman 图，通过测量误差评估一致性：不同时间点的重复测量次数从 436 次到 474 次不等。不同牙齿组和不同时间点的测量结果表明，测量者之间和测量者内部的可靠性良好到极佳。测量误差介于 0.41 毫米和 0.77 毫米之间。布兰德-阿尔特曼图的 95% 一致性限值在+1.43 毫米和-2.01 毫米之间，适用于不同的牙齿组和时间点：这项研究的结果表明，在正畸治疗的不同阶段，使用不同机器的 MPR 进行 CBCT 是一种可重复的测量牙根长度的方法。当出于临床或研究目的解释使用 MPR 进行的 CBCT 牙根缩短测量值时，应谨慎对待低于 2 毫米的值，因为它们可能包含测量误差。

{"title":"Reliability and agreement of root length measurements during orthodontic treatment in images from different CBCT machines using multiplanar reconstruction.","authors":"Kristina Johansson, Liselotte Paulsson, Helena Christell","doi":"10.2340/biid.v11.41161","DOIUrl":"10.2340/biid.v11.41161","url":null,"abstract":"Objectives: To assess inter- and intrarater reliability and agreement for measurements of root lengths using multiplanar reconstruction (MPR) in cone beam computed tomography (CBCT) examinations.Furthermore, to determine whether using MPR from different CBCT machines was a reliable and reproducible method for assessment of root length during orthodontic treatment of adolescents.Materials and methods: A total of 40 CBCT examinations obtained before, during and after orthodontic treatment of 14 adolescents, with fixed appliances from a multicentre randomised controlled trial, were used. All roots from the incisors to the first molars were measured by two independent raters and in accordance with a protocol preceded by a multi-step calibration. Reliability was assessed by intra class correlation (ICC). Agreement was assessed by measurement error according to the Dahlberg formula and Bland-Altman plot.Results: The number of repeated measurements varied from 436 to 474 for the different timepoints. Good to excellent inter- and intrarater reliability for different tooth groups and timepoints were shown. Measurement error for inter- and intrarater agreement varied between 0.41 mm and 0.77 mm. The Bland-Altman plot with 95% limits of agreement varied between +1.43 mm and -2.01 mm for different tooth groups and timepoints.Conclusions: The results of this study indicate that CBCT using MPR from different machines is a reproducible method for measuring root length during different phases of orthodontic treatment. When interpreting root shortening measurements in CBCT using MPR for clinical or research purposes, values below 2 mm should be approached with caution, as they may contain measurement errors.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11371306/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142127525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recipient of Biomaterial Investigations in Dentistry's Young Author Award 2023. 荣获 2023 年牙科生物材料研究青年作者奖。

Biomaterial investigations in dentistry

Pub Date : 2024-08-22 eCollection Date: 2024-01-01 DOI: 10.2340/biid.v11.41367

Anne Peutzfeldt, Jon E Dahl

引用次数: 0

The sealing ability of different endodontic biomaterials as an intra-orifice barrier: evaluation with high-performance liquid chromatography. 不同牙髓生物材料作为孔内屏障的密封能力：用高效液相色谱法进行评估。

Biomaterial investigations in dentistry

Pub Date : 2024-07-24 eCollection Date: 2024-01-01 DOI: 10.2340/biid.v11.41069

Sahar Shakouei, Negin Ghasemi, Parvin Zakeri-Milani, Afsaneh Shahali, Mahdieh Alipour

Objective: This study evaluated the sealing ability of different biomaterials as intra-orifice barriers in the internal bleaching of discolored teeth with the walking bleaching technique. The release of hydroxyl ions from the bleaching materials can cause cervical root resorption, making it necessary to use intra-orifice barrier materials to prevent this issue.

Materials and methods: In the current study, the high-performance liquid chromatography (HPLC) method was used to measure the released hydroxyl ions. The study included 90 single-rooted and single-canal premolars, which were divided into four groups based on the intra-orifice barrier materials used (mineral trioxide aggregate [MTA], calcium-enriched mixture [CEM], Biodentine, and MTA+PG) and the type of bleaching material (sodium perborate + water or sodium perborate + hydrogen peroxide 30%). Two control groups were also considered in this study: a positive control group, where sodium perborate and hydrogen peroxide were placed inside the pulp chamber without any intra-orifice barriers; and a negative control group, where no bleaching agent or surgical obstruction was used, and the root surface was covered with wax up to the cemento-enamel junction (CEJ) level.

Results: The results showed that there was a significant difference in the concentration of hydroxyl ions released among the studied groups. The amount of hydroxyl ion released was highest in the positive control group and lowest in the CEM group. Among the intra-orifice barrier materials used, CEM cement was found to be the most appropriate material for use in the step-by-step internal bleaching method.

Conclusions: The study highlights the importance of using appropriate intra-orifice barrier materials to prevent root cervical resorption in internal bleaching procedures.

研究目的本研究评估了不同的生物材料作为孔内屏障在步行漂白技术内漂白变色牙时的封闭能力。漂白材料释放的羟基离子会导致牙颈部牙根吸收，因此有必要使用孔内屏障材料来防止这一问题：本研究采用高效液相色谱法（HPLC）测量释放的羟基离子。研究包括 90 颗单根和单冠前臼齿，根据使用的口内屏障材料（三氧化物矿物骨料 [MTA]、富钙混合物 [CEM]、Biodentine 和 MTA+PG）和漂白材料类型（过硼酸钠 + 水或过硼酸钠 + 30% 过氧化氢）分为四组。本研究还考虑了两个对照组：一个是阳性对照组，即在牙髓腔内放置过硼酸钠和过氧化氢，不设置任何孔内障碍；另一个是阴性对照组，即不使用漂白剂或手术障碍，根面用蜡覆盖至牙本质-釉质交界处（CEJ）水平：结果表明，各研究组释放的羟基离子浓度存在显著差异。阳性对照组释放的羟基离子量最高，CEM 组最低。在所用的孔内屏障材料中，CEM 水泥被认为是最适合用于分步内漂白法的材料：该研究强调了在内侧漂白过程中使用适当的口内屏障材料来防止牙根颈吸收的重要性。

{"title":"The sealing ability of different endodontic biomaterials as an intra-orifice barrier: evaluation with high-performance liquid chromatography.","authors":"Sahar Shakouei, Negin Ghasemi, Parvin Zakeri-Milani, Afsaneh Shahali, Mahdieh Alipour","doi":"10.2340/biid.v11.41069","DOIUrl":"10.2340/biid.v11.41069","url":null,"abstract":"Objective: This study evaluated the sealing ability of different biomaterials as intra-orifice barriers in the internal bleaching of discolored teeth with the walking bleaching technique. The release of hydroxyl ions from the bleaching materials can cause cervical root resorption, making it necessary to use intra-orifice barrier materials to prevent this issue.Materials and methods: In the current study, the high-performance liquid chromatography (HPLC) method was used to measure the released hydroxyl ions. The study included 90 single-rooted and single-canal premolars, which were divided into four groups based on the intra-orifice barrier materials used (mineral trioxide aggregate [MTA], calcium-enriched mixture [CEM], Biodentine, and MTA+PG) and the type of bleaching material (sodium perborate + water or sodium perborate + hydrogen peroxide 30%). Two control groups were also considered in this study: a positive control group, where sodium perborate and hydrogen peroxide were placed inside the pulp chamber without any intra-orifice barriers; and a negative control group, where no bleaching agent or surgical obstruction was used, and the root surface was covered with wax up to the cemento-enamel junction (CEJ) level.Results: The results showed that there was a significant difference in the concentration of hydroxyl ions released among the studied groups. The amount of hydroxyl ion released was highest in the positive control group and lowest in the CEM group. Among the intra-orifice barrier materials used, CEM cement was found to be the most appropriate material for use in the step-by-step internal bleaching method.Conclusions: The study highlights the importance of using appropriate intra-orifice barrier materials to prevent root cervical resorption in internal bleaching procedures.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11273217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141790174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro study on the influence of laser-activated irrigation on infiltration and leakage of a dual curing-resin cement as an endodontic sealer 关于激光激活灌洗对作为牙髓密封剂的双固化树脂水门汀的渗透和渗漏影响的体外研究

Biomaterial investigations in dentistry

Pub Date : 2024-07-12 DOI: 10.2340/biid.v11.41098

L. Marger, M. Abdelaziz, E. Bella, I. Krejci, C. I. Anton Y Otero

Objectives: The study aims to investigate the effects of laser-activated irrigation on infiltration and microleakage of a dual-curing resin cement applied as a root canal sealer.Methods: Thirty-eight extracted upper molars were attributed to four experimental groups. Roots were mechanically enlarged and disinfected (NaOCl). Control samples (n = 11) were irrigated with conventional needles and three different lasers were used to activate the irrigant in the other groups (n = 9): 2.94 µm Er:YAG laser, 9,300 µm CO2 laser and 808 nm diode laser with a modified black coated laser tip. Final irrigation was performed in each group with ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl) and sodium chloride (NaCl) activated with lasers and in the control without activation.Dentin tubules were then labeled with a red fluorophore (Rhodamine B) and the root canals were sealed with a dual-curing resin cement (Paracore). The cement fixed the dye on the sealed and infiltrated dentin parts. To remove the Rhodamine not fixed by the cement, roots were then sectioned horizontally and immersed in hydrogen peroxide (H2O2). The empty dentin tubules spaces were then labeled with a green fluorophore (Fluorescein) allowing the visualization of infiltration and microleakage by confocal microscopy.Results: Percentages of infiltration were significantly higher in the middle root third of the control and Er:YAG laser-activated samples compared to CO2 or diode laser groups. Microleakage was present in all experimental groups but significantly less after CO2 laser activation.Conclusion: Laser-activated irrigation impacted resin cement infiltration and microleakage dependent on the applied wavelength. Er:YAG laser activation led to higher values of infiltration and microleakage compared to CO2 and diode lasers.

研究目的本研究旨在探讨激光激活灌洗对用作根管封闭剂的双固化树脂水泥的渗透和微渗漏的影响：方法：38 颗拔出的上磨牙分为四个实验组。对牙根进行机械扩大和消毒（NaOCl）。对照组样本（n = 11）使用传统针头灌洗，其他组样本（n = 9）使用三种不同的激光激活灌洗剂：2.94 µm Er:YAG 激光器、9,300 µm CO2 激光器和 808 nm 带有改良黑色涂层激光器尖端的二极管激光器。然后用红色荧光团（罗丹明 B）标记牙本质小管，并用双固化树脂水门汀（Paracore）密封根管。然后用红色荧光团（罗丹明 B）标记牙本质小管，再用双固化树脂水门汀（Paracore）密封根管。为了去除未被粘接剂固定的罗丹明，将牙根水平切片并浸泡在过氧化氢（H2O2）中。然后用绿色荧光团（荧光素）标记空的牙本质小管空间，这样就能通过共聚焦显微镜观察到浸润和微渗漏：结果：与 CO2 或二极管激光组相比，对照组和 Er:YAG 激光激活组样品中三分之一根部的浸润比例明显更高。所有实验组都存在微渗漏，但 CO2 激光激活后微渗漏明显减少：结论：激光激活灌注对树脂粘结剂渗透和微渗漏的影响取决于所使用的波长。与 CO2 和二极管激光相比，Er:YAG 激光激活导致的浸润和微渗漏值更高。

{"title":"An in vitro study on the influence of laser-activated irrigation on infiltration and leakage of a dual curing-resin cement as an endodontic sealer","authors":"L. Marger, M. Abdelaziz, E. Bella, I. Krejci, C. I. Anton Y Otero","doi":"10.2340/biid.v11.41098","DOIUrl":"https://doi.org/10.2340/biid.v11.41098","url":null,"abstract":"Objectives: The study aims to investigate the effects of laser-activated irrigation on infiltration and microleakage of a dual-curing resin cement applied as a root canal sealer.\u0000Methods: Thirty-eight extracted upper molars were attributed to four experimental groups. Roots were mechanically enlarged and disinfected (NaOCl). Control samples (n = 11) were irrigated with conventional needles and three different lasers were used to activate the irrigant in the other groups (n = 9): 2.94 µm Er:YAG laser, 9,300 µm CO2 laser and 808 nm diode laser with a modified black coated laser tip. Final irrigation was performed in each group with ethylenediaminetetraacetic acid (EDTA), sodium hypochlorite (NaOCl) and sodium chloride (NaCl) activated with lasers and in the control without activation.Dentin tubules were then labeled with a red fluorophore (Rhodamine B) and the root canals were sealed with a dual-curing resin cement (Paracore). The cement fixed the dye on the sealed and infiltrated dentin parts. To remove the Rhodamine not fixed by the cement, roots were then sectioned horizontally and immersed in hydrogen peroxide (H2O2). The empty dentin tubules spaces were then labeled with a green fluorophore (Fluorescein) allowing the visualization of infiltration and microleakage by confocal microscopy.\u0000Results: Percentages of infiltration were significantly higher in the middle root third of the control and Er:YAG laser-activated samples compared to CO2 or diode laser groups. Microleakage was present in all experimental groups but significantly less after CO2 laser activation.\u0000Conclusion: Laser-activated irrigation impacted resin cement infiltration and microleakage dependent on the applied wavelength. Er:YAG laser activation led to higher values of infiltration and microleakage compared to CO2 and diode lasers.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141653150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Accumulation and removal of Streptococcus mutans biofilm on enamel and root surfaces in vitro 变异链球菌生物膜在牙釉质和牙根表面的体外积累和清除

Biomaterial investigations in dentistry

Pub Date : 2024-07-12 DOI: 10.2340/biid.v11.41059

Anne Breivik, Aida Mulic, A. Sehic, H. Valen, S. Kopperud, Linda Stein, Qalbi Khan

Objective: This study aimed to quantitatively investigate the accumulation of Streptococcus mutans biofilm on enamel and root surfaces and assess the amount of biofilm removal using (1) experimental toothpaste and (2) water, in a closed system of flow chamber.Methods: Eight sound premolars were embedded in epoxy resin and polished with silicon carbide grinding papers to display enamel and root surfaces. To mimic biofilm, cultures of Streptococcus mutans were prepared and grown on the tooth surfaces over night before they were exposed to either 2 liters of Milli Q water or 2 liters of 40% experimental toothpaste in the flow chamber. The amount of biofilm was measured and quantified in Fluorescence microscopy. Mean fluorescence values were recorded and analysed using Microsoft® Excel® (MS Excel 2016).Results: The ability to grow biofilm was equally present at both the enamel and root surfaces. The use of water and 40% experimental toothpaste showed a significant reduction of areas covered with biofilm on both enamel and root dentin in comparison to untreated surfaces (p < 0.01). Significantly more biofilm was removed from enamel compared to root surfaces when treated with either water and toothpaste (p < 0.01). Slightly less biofilm was removed by the use of water compared to toothpaste on both enamel and root dentin surfaces, although the differences were not statistically significant.Conclusion: The results indicate that less biofilm is removed from the root surfaces than enamel by the use of water and 40% experimental toothpaste in flow chamber. Assessing oral biofilm accumulation and monitoring biofilm formation on enamel and root dentin surfaces give oral health professionals important directions that could strenghten the significance of dental caries prevention. Improving older individuals’ oral hygiene practices should therefore be considered an important measure to prevent root caries.

研究目的本研究旨在定量研究变异链球菌生物膜在牙釉质和牙根表面的积累情况，并评估在封闭的流动室系统中使用（1）实验牙膏和（2）水对生物膜的清除量：将 8 颗健全的前臼齿嵌入环氧树脂中，用碳化硅研磨纸抛光，以显示牙釉质和牙根表面。为了模拟生物膜，先制备变异链球菌培养物并在牙齿表面培养过夜，然后将其置于流动室中的 2 升 Milli Q 水或 2 升 40% 实验牙膏中。生物膜的数量通过荧光显微镜进行测量和量化。使用 Microsoft® Excel® （MS Excel 2016）记录和分析平均荧光值：结果：生物膜在牙釉质和牙根表面的生长能力相同。与未经处理的表面相比，使用水和 40% 实验牙膏可显著减少牙釉质和牙本质根部被生物膜覆盖的面积（p < 0.01）。与牙根表面相比，使用水和牙膏处理的牙釉质表面去除的生物膜明显较多（p < 0.01）。在牙釉质和牙根牙本质表面，使用水和牙膏去除的生物膜略少，但差异无统计学意义：结果表明，在流动室中使用水和 40% 的实验牙膏去除牙根表面的生物膜少于去除牙釉质的生物膜。评估口腔生物膜的积累情况以及监测牙釉质和牙根表面生物膜的形成，为口腔卫生专业人员提供了重要的方向，可以加强龋齿预防的意义。因此，改善老年人的口腔卫生习惯应被视为预防根龋的一项重要措施。

{"title":"Accumulation and removal of Streptococcus mutans biofilm on enamel and root surfaces in vitro","authors":"Anne Breivik, Aida Mulic, A. Sehic, H. Valen, S. Kopperud, Linda Stein, Qalbi Khan","doi":"10.2340/biid.v11.41059","DOIUrl":"https://doi.org/10.2340/biid.v11.41059","url":null,"abstract":"Objective: This study aimed to quantitatively investigate the accumulation of Streptococcus mutans biofilm on enamel and root surfaces and assess the amount of biofilm removal using (1) experimental toothpaste and (2) water, in a closed system of flow chamber.\u0000Methods: Eight sound premolars were embedded in epoxy resin and polished with silicon carbide grinding papers to display enamel and root surfaces. To mimic biofilm, cultures of Streptococcus mutans were prepared and grown on the tooth surfaces over night before they were exposed to either 2 liters of Milli Q water or 2 liters of 40% experimental toothpaste in the flow chamber. The amount of biofilm was measured and quantified in Fluorescence microscopy. Mean fluorescence values were recorded and analysed using Microsoft® Excel® (MS Excel 2016).\u0000Results: The ability to grow biofilm was equally present at both the enamel and root surfaces. The use of water and 40% experimental toothpaste showed a significant reduction of areas covered with biofilm on both enamel and root dentin in comparison to untreated surfaces (p < 0.01). Significantly more biofilm was removed from enamel compared to root surfaces when treated with either water and toothpaste (p < 0.01). Slightly less biofilm was removed by the use of water compared to toothpaste on both enamel and root dentin surfaces, although the differences were not statistically significant.\u0000Conclusion: The results indicate that less biofilm is removed from the root surfaces than enamel by the use of water and 40% experimental toothpaste in flow chamber. Assessing oral biofilm accumulation and monitoring biofilm formation on enamel and root dentin surfaces give oral health professionals important directions that could strenghten the significance of dental caries prevention. Improving older individuals’ oral hygiene practices should therefore be considered an important measure to prevent root caries.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141654108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study 探究钛颗粒大小和浓度对人类成骨细胞成骨反应的影响--体外研究

Biomaterial investigations in dentistry

Pub Date : 2024-06-13 DOI: 10.2340/biid.v11.40843

S. Sheela, Waad Kheder, Rani Samsudin

Purpose: The purpose of this study was to investigate the correlation between the size and concentration of titanium particles and the osteogenic response of human osteoblasts (HOB). Materials and Methods: Different concentrations of titanium dioxide nano- and micro-particles were prepared and their biocompatibility on HOBs was analyzed using XTT assay. The changes in the actin cytoskeletal organization were studied by confocal laser scanning microscopy. The generation of intracellular reactive oxygen species (ROS) by HOBs after exposure to titanium dioxide particles was analyzed using ROS assay. Besides, the osteogenic potential represented by alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels, and biomineralization were analyzed.Results: Short-term interaction of titanium dioxide nano- and micro-particles did not induce toxicity to HOBs. However, cells treated with 100 μg/mL titanium dioxide nano- and micro-particles demonstrated higher ROS generation compared to control. Besides, cells treated with 100 µg/mL titanium dioxide nanoparticles showed higher alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels and biomineralization compared to titanium dioxide microparticles. Conclusion: Collectively, the study found titanium dioxide nanoparticles to be more biocompatible than microparticles providing an insight into the capability of nanostructures in supporting osteoblast differentiation and its plausibility in biomedical applications.

目的：本研究旨在探讨钛颗粒的大小和浓度与人成骨细胞（HOB）成骨反应之间的相关性。材料与方法：制备不同浓度的纳米和微米二氧化钛颗粒，并采用 XTT 法分析其对 HOB 的生物相容性。共聚焦激光扫描显微镜研究了肌动蛋白细胞骨架组织的变化。使用 ROS 分析法分析了 HOBs 暴露于二氧化钛颗粒后细胞内活性氧（ROS）的生成情况。此外，还分析了以碱性磷酸酶活性、骨蛋白激酶、巨噬细胞集落刺激因子水平和生物矿化为代表的成骨潜能：结果：二氧化钛纳米颗粒和微颗粒的短期相互作用不会对 HOBs 产生毒性。然而，与对照组相比，用 100 微克/毫升的纳米二氧化钛和微颗粒处理的细胞会产生更多的 ROS。此外，与二氧化钛微颗粒相比，用 100 微克/毫升二氧化钛纳米颗粒处理的细胞显示出更高的碱性磷酸酶活性、骨保护素、巨噬细胞集落刺激因子水平和生物矿化度。结论总之，该研究发现二氧化钛纳米颗粒比微颗粒更具生物相容性，从而深入了解了纳米结构支持成骨细胞分化的能力及其在生物医学应用中的可行性。

{"title":"Investigating the influence of titanium particle size and concentration on osteogenic response of human osteoblasts – in vitro study","authors":"S. Sheela, Waad Kheder, Rani Samsudin","doi":"10.2340/biid.v11.40843","DOIUrl":"https://doi.org/10.2340/biid.v11.40843","url":null,"abstract":"Purpose: The purpose of this study was to investigate the correlation between the size and concentration of titanium particles and the osteogenic response of human osteoblasts (HOB). \u0000Materials and Methods: Different concentrations of titanium dioxide nano- and micro-particles were prepared and their biocompatibility on HOBs was analyzed using XTT assay. The changes in the actin cytoskeletal organization were studied by confocal laser scanning microscopy. The generation of intracellular reactive oxygen species (ROS) by HOBs after exposure to titanium dioxide particles was analyzed using ROS assay. Besides, the osteogenic potential represented by alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels, and biomineralization were analyzed.\u0000Results: Short-term interaction of titanium dioxide nano- and micro-particles did not induce toxicity to HOBs. However, cells treated with 100 μg/mL titanium dioxide nano- and micro-particles demonstrated higher ROS generation compared to control. Besides, cells treated with 100 µg/mL titanium dioxide nanoparticles showed higher alkaline phosphatase activity, osteoprotegerin, macrophage colony stimulating factor levels and biomineralization compared to titanium dioxide microparticles. \u0000Conclusion: Collectively, the study found titanium dioxide nanoparticles to be more biocompatible than microparticles providing an insight into the capability of nanostructures in supporting osteoblast differentiation and its plausibility in biomedical applications.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141349645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biological properties versus solubility of endodontic sealers and cements 牙髓密封剂和水门汀的生物特性与可溶性

Biomaterial investigations in dentistry

Pub Date : 2024-06-04 DOI: 10.2340/biid.v11.40863

Inge Fristad, Sivakami Haug, A. Bårdsen

Endodontic sealers and cements used in root canal treatment have different compositions and properties. Common to all materials is that their primary goal is to fill gaps and voids, making a permanent seal of the root canal system. Furthermore, aspects such as antibacterial properties, cytotoxicity, setting time, solubility and biocompatibility are also crucial and ought to be considered. Over the years, a shift in the view on the importance of these aspects has ocurred. Whereas the antibacterial properties were considered important when the technical factors in endodontics were less developed, the sealing ability and biocompatibility have later been considered the most critical factors. The introduction of tricalcium silicate cements and sealers has led to a renewed interest in material properties, as these cements seem to have good sealing ability and at the same time combine favourable antimicrobial effects with excellent biocompatibility. This review discusses how the various properties of root canal sealers and cements may conflict with the primary aim of providing a permanent seal of the root canal system.

根管治疗中使用的根管封闭剂和水泥具有不同的成分和特性。所有材料的共同点是，它们的主要目的是填补缝隙和空隙，对根管系统进行永久性密封。此外，抗菌性、细胞毒性、凝固时间、溶解性和生物相容性等方面也至关重要，必须加以考虑。多年来，人们对这些方面的重要性的看法发生了转变。在牙髓技术还不发达的时候，人们认为抗菌性能很重要，而后来则认为密封能力和生物相容性是最关键的因素。硅酸三钙水门汀和封闭剂的问世再次引起了人们对材料特性的关注，因为这些水门汀似乎具有良好的封闭能力，同时兼具良好的抗菌效果和出色的生物相容性。本综述将讨论根管封闭剂和水门汀的各种特性如何与提供根管系统永久封闭的主要目的相冲突。

引用次数: 0

An in vitro evaluation of endodontic sealers and an antibiotic to assess their antimicrobial effect against Enterococcus faecalis 体外评估牙髓密封剂和抗生素对粪肠球菌的抗菌效果

Biomaterial investigations in dentistry

Pub Date : 2024-05-24 DOI: 10.2340/biid.v11.40646

Elizabeth Madla-Cruz, Vanascheck Dasaev Villanueva-Pérez, Myriam A. De la Garza-Ramos, J. Flores-Treviño, Idalia Rodríguez-Delgado, Fanny López-Martinez

ObjectiveThis study aimed to compare the antimicrobial effect of three endodontic sealers (AH Plus, Mineral trioxide aggregate [MTA] Fillapex, and BioRoot RCS) with and without amoxicillin against E. faecalis. MethodologyAmoxicillin, equivalent to 10% of the sealers’ total weight, was mixed with the sealers. Another batch was prepared without amoxicillin. The direct contact test (DCT) and the agar diffusion test were used to assess the antibacterial effect. Results were analysed using one-way analysis of variance (ANOVA), the F-test, and the Kruskal-Wallis test. ResultsAH Plus significantly suppressed E. faecalis without the addition of amoxicillin in the DCT (p = 0.011), while in the agar diffusion test, BioRoot RCS had a larger inhibition zone than the control (p < 0.001). When amoxicillin was added to the sealers, AH Plus (p = 0.003) and MTA Fillapex (p = 0.042) reduced E. faecalis growth. In contrast, all three sealers showed larger inhibition zones than the control (p = 0.001), with AH Plus displaying a larger inhibition zone than MTA Fillapex (p = 0.042) and BioRoot RCS (p = 0.032). ConclusionsIt was thus concluded that the addition of amoxicillin to endodontic sealers enhances their antimicrobial activity against E. faecalis.

目的本研究旨在比较三种牙髓封闭剂（AH Plus、Mineral trioxide aggregate [MTA] Fillapex 和 BioRoot RCS）添加和不添加阿莫西林对粪肠球菌的抗菌效果。方法将相当于封闭剂总重量 10%的阿莫西林与封闭剂混合。另一批密封剂不含阿莫西林。采用直接接触试验（DCT）和琼脂扩散试验来评估抗菌效果。结果采用单因素方差分析（ANOVA）、F 检验和 Kruskal-Wallis 检验进行分析。在 DCT 中，ResultsAH Plus 在不添加阿莫西林的情况下能明显抑制粪肠球菌（p = 0.011），而在琼脂扩散试验中，BioRoot RCS 的抑制区大于对照组（p < 0.001）。在封闭剂中添加阿莫西林后，AH Plus（p = 0.003）和 MTA Fillapex（p = 0.042）可减少粪肠球菌的生长。相比之下，三种封闭剂的抑制区都大于对照组（p = 0.001），其中 AH Plus 的抑制区大于 MTA Fillapex（p = 0.042）和 BioRoot RCS（p = 0.032）。结论因此可以得出结论，在牙髓封闭剂中添加阿莫西林可以增强其对粪大肠杆菌的抗菌活性。

{"title":"An in vitro evaluation of endodontic sealers and an antibiotic to assess their antimicrobial effect against Enterococcus faecalis","authors":"Elizabeth Madla-Cruz, Vanascheck Dasaev Villanueva-Pérez, Myriam A. De la Garza-Ramos, J. Flores-Treviño, Idalia Rodríguez-Delgado, Fanny López-Martinez","doi":"10.2340/biid.v11.40646","DOIUrl":"https://doi.org/10.2340/biid.v11.40646","url":null,"abstract":"ObjectiveThis study aimed to compare the antimicrobial effect of three endodontic sealers (AH Plus, Mineral trioxide aggregate [MTA] Fillapex, and BioRoot RCS) with and without amoxicillin against E. faecalis. \u0000MethodologyAmoxicillin, equivalent to 10% of the sealers’ total weight, was mixed with the sealers. Another batch was prepared without amoxicillin. The direct contact test (DCT) and the agar diffusion test were used to assess the antibacterial effect. Results were analysed using one-way analysis of variance (ANOVA), the F-test, and the Kruskal-Wallis test. \u0000ResultsAH Plus significantly suppressed E. faecalis without the addition of amoxicillin in the DCT (p = 0.011), while in the agar diffusion test, BioRoot RCS had a larger inhibition zone than the control (p < 0.001). When amoxicillin was added to the sealers, AH Plus (p = 0.003) and MTA Fillapex (p = 0.042) reduced E. faecalis growth. In contrast, all three sealers showed larger inhibition zones than the control (p = 0.001), with AH Plus displaying a larger inhibition zone than MTA Fillapex (p = 0.042) and BioRoot RCS (p = 0.032). \u0000ConclusionsIt was thus concluded that the addition of amoxicillin to endodontic sealers enhances their antimicrobial activity against E. faecalis.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141099550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The effect of orthodontic bracket base shape on shear bond strength to human enamel, an in vitro study 正畸托槽基底形状对人体珐琅质剪切粘接强度的影响，体外研究

Biomaterial investigations in dentistry

Pub Date : 2024-05-07 DOI: 10.2340/biid.v11.40585

Ziana Esmail, William Wiltshire, Fabio H. S. L. Pinheiro, C. M. Frota, Rodrigo França

The purpose of this study was to evaluate the in vitro effect of orthodontic bracket base shape on shear bond strength (SBS) to human enamel and assess the nature of debonding fractures using the Adhesive Remnant Index (ARI). Orthodontic brackets with different-shaped bases (flower, heart, rectangle) were bonded to 120 extracted human third molars. Shear bond strength was measured using a Servohydraulic Test System at 24 h and 2 months after bonding. Adhesive Remnant Index scores were evaluated under 10x magnification to assess the amount of resin left on the tooth. The control bracket (rectangular base shape) had the highest mean SBS (26.8 ± 8.2 megapascals [MPa]), and significantly differed from the flower (17.2 ± 4.4 MPa) and heart (18.9 ± 3.5 MPa) base shapes (p < 0.001). The mean SBS between debonding times at 24 h (21.5 ± 7.4 MPa) and 2 months (20.4 ± 6.7 MPa) were not statistically significant (p > 0.05). Analysis of ARI scores showed a significant difference between flower-24 h versus heart-2 months (p = 0.039), flower-24 h versus heart-24 h (p = 0.004), and control-2 months versus heart-24 h (p = 0.015). Bracket base shape influenced SBS, with the rectangular base shape having a higher mean SBS compared to flower and heart base shapes. Variations in ARI scores occurred based on bracket shape and were of a mixed adhesive-cohesive nature. All bracket shapes had bond strengths above the clinically acceptable range of 6–8 MPa, and may thus provide adequate SBS in a clinical situation.

本研究旨在体外评估正畸托槽基底形状对人体珐琅质剪切粘结强度（SBS）的影响，并使用粘合剂残留指数（ARI）评估脱粘断裂的性质。在 120 颗拔出的人类第三磨牙上粘结了不同形状基托（花、心、矩形）的正畸托槽。粘结后 24 小时和 2 个月时，使用伺服液压测试系统测量剪切粘结强度。在 10 倍放大镜下评估粘合剂残留指数，以评估牙齿上残留的树脂量。对照托槽（矩形基底形状）的平均 SBS（26.8 ± 8.2 兆帕 [MPa]）最高，与花形（17.2 ± 4.4 MPa）和心形（18.9 ± 3.5 MPa）基底形状有显著差异（p < 0.001）。24 小时（21.5 ± 7.4 兆帕）和 2 个月（20.4 ± 6.7 兆帕）脱粘时间之间的平均 SBS 没有统计学意义（p > 0.05）。对 ARI 分数的分析表明，花朵-24 小时与心脏-2 个月相比（p = 0.039）、花朵-24 小时与心脏-24 小时相比（p = 0.004）以及对照-2 个月与心脏-24 小时相比（p = 0.015）差异显著。支架底座形状对 SBS 有影响，矩形底座形状的平均 SBS 高于花形和心形底座形状的平均 SBS。支架形状不同，ARI 分数也不同，而且粘附性和粘合性参差不齐。所有支架形状的粘接强度都高于临床可接受的 6-8 兆帕的范围，因此在临床情况下可以提供足够的 SBS。

{"title":"The effect of orthodontic bracket base shape on shear bond strength to human enamel, an in vitro study","authors":"Ziana Esmail, William Wiltshire, Fabio H. S. L. Pinheiro, C. M. Frota, Rodrigo França","doi":"10.2340/biid.v11.40585","DOIUrl":"https://doi.org/10.2340/biid.v11.40585","url":null,"abstract":"The purpose of this study was to evaluate the in vitro effect of orthodontic bracket base shape on shear bond strength (SBS) to human enamel and assess the nature of debonding fractures using the Adhesive Remnant Index (ARI). Orthodontic brackets with different-shaped bases (flower, heart, rectangle) were bonded to 120 extracted human third molars. Shear bond strength was measured using a Servohydraulic Test System at 24 h and 2 months after bonding. Adhesive Remnant Index scores were evaluated under 10x magnification to assess the amount of resin left on the tooth. The control bracket (rectangular base shape) had the highest mean SBS (26.8 ± 8.2 megapascals [MPa]), and significantly differed from the flower (17.2 ± 4.4 MPa) and heart (18.9 ± 3.5 MPa) base shapes (p < 0.001). The mean SBS between debonding times at 24 h (21.5 ± 7.4 MPa) and 2 months (20.4 ± 6.7 MPa) were not statistically significant (p > 0.05). Analysis of ARI scores showed a significant difference between flower-24 h versus heart-2 months (p = 0.039), flower-24 h versus heart-24 h (p = 0.004), and control-2 months versus heart-24 h (p = 0.015). Bracket base shape influenced SBS, with the rectangular base shape having a higher mean SBS compared to flower and heart base shapes. Variations in ARI scores occurred based on bracket shape and were of a mixed adhesive-cohesive nature. All bracket shapes had bond strengths above the clinically acceptable range of 6–8 MPa, and may thus provide adequate SBS in a clinical situation. ","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141003929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of different beverages and polishing techniques on colour stability of CAD/CAM composite restorative materials. 不同饮料和抛光技术对 CAD/CAM 复合修复材料颜色稳定性的影响。

Biomaterial investigations in dentistry

Pub Date : 2024-04-26 eCollection Date: 2024-01-01 DOI: 10.2340/biid.v11.40591

Lippo Lassila, Mine B Uctasli, Kanae Wada, Pekka K Vallittu, Sufyan Garoushi

Objectives: The aim of this article was to compare the colour stability of short fibre-reinforced computer-assisted design/computer-assisted manufacturing (CAD/CAM) composite (SFRC CAD) to commercially available CAD/CAM materials following prolonged immersion in a variety of beverages. Furthermore, the influence of the polishing technique was evaluated.

Materials and methods: A total of 120 rectangular specimens (10 mm length × 14 mm width × 2 mm thickness) were prepared from SFRC CAD, IPS e-max, Cerasmart 270, Celtra Duo, Enamic, and Brilliant Crios. The specimens underwent polishing through either a laboratory polishing machine equipped with 4000-grit silicon carbide paper or chairside polishing using Sof-Lex spiral. Twenty specimens of each tested CAD/CAM material were randomly divided into four groups (n = 5) based on the staining solution used in order to evaluate the colour stability of the materials. Group 1: distilled water, Group 2: coffee, Group 3: red wine, Group 4: coke. Using a spectrophotometer, the colour changes (∆E) of all CAD/CAM materials were assessed at baseline, and after 1 and 12 weeks of staining. Three-way analysis of variance was used to analyse the data (α = 0.05).

Results: The staining solution and material type showed a significant influence on the CAD/CAM specimens' colour stability (p < 0.05), while polishing method had no significant influence (p > 0.05). The average ∆E values for specimens submerged in wine were considerably higher (p < 0.05) than those for the other solutions. SFRC CAD, Cerasmart 270, and Enamic displayed the highest ∆E values in wine (p < 0.05).

Conclusions: The colour stability of tested SFRC CAD was comparable to other composite-based CAD/CAM materials, while IPS e.max exhibited the highest level of colour stability.

目的：本文旨在比较短纤维增强计算机辅助设计/计算机辅助制造（CAD/CAM）复合材料（SFRC CAD）与市售 CAD/CAM 材料在各种饮料中长期浸泡后的颜色稳定性。此外，还评估了抛光技术的影响：从 SFRC CAD、IPS e-max、Cerasmart 270、Celtra Duo、Enamic 和 Brilliant Crios 中制备了 120 个矩形试样（长 10 毫米×宽 14 毫米×厚 2 毫米）。试样通过装有 4000 级碳化硅纸的实验室抛光机或使用 Sof-Lex 螺旋抛光机进行抛光。为了评估材料的颜色稳定性，每种受测 CAD/CAM 材料的 20 个试样根据所使用的染色液随机分为四组（n = 5）。第一组：蒸馏水，第二组：咖啡，第三组：红酒，第四组：可乐。使用分光光度计评估所有 CAD/CAM 材料在基线以及染色 1 周和 12 周后的颜色变化（ΔE）。结果显示，染色溶液和材料类型显示了不同的颜色变化：结果：染色液和材料类型对 CAD/CAM 试样的颜色稳定性有显著影响（p < 0.05），而抛光方法没有显著影响（p > 0.05）。浸没在葡萄酒中的试样的平均 ∆E 值明显高于其他溶液（p < 0.05）。SFRC CAD、Cerasmart 270 和 Enamic 在葡萄酒中的 ∆E 值最高（p < 0.05）：经测试的 SFRC CAD 的颜色稳定性与其他基于复合材料的 CAD/CAM 材料相当，而 IPS e.max 的颜色稳定性水平最高。

{"title":"Effect of different beverages and polishing techniques on colour stability of CAD/CAM composite restorative materials.","authors":"Lippo Lassila, Mine B Uctasli, Kanae Wada, Pekka K Vallittu, Sufyan Garoushi","doi":"10.2340/biid.v11.40591","DOIUrl":"10.2340/biid.v11.40591","url":null,"abstract":"Objectives: The aim of this article was to compare the colour stability of short fibre-reinforced computer-assisted design/computer-assisted manufacturing (CAD/CAM) composite (SFRC CAD) to commercially available CAD/CAM materials following prolonged immersion in a variety of beverages. Furthermore, the influence of the polishing technique was evaluated.Materials and methods: A total of 120 rectangular specimens (10 mm length × 14 mm width × 2 mm thickness) were prepared from SFRC CAD, IPS e-max, Cerasmart 270, Celtra Duo, Enamic, and Brilliant Crios. The specimens underwent polishing through either a laboratory polishing machine equipped with 4000-grit silicon carbide paper or chairside polishing using Sof-Lex spiral. Twenty specimens of each tested CAD/CAM material were randomly divided into four groups (n = 5) based on the staining solution used in order to evaluate the colour stability of the materials. Group 1: distilled water, Group 2: coffee, Group 3: red wine, Group 4: coke. Using a spectrophotometer, the colour changes (∆E) of all CAD/CAM materials were assessed at baseline, and after 1 and 12 weeks of staining. Three-way analysis of variance was used to analyse the data (α = 0.05).Results: The staining solution and material type showed a significant influence on the CAD/CAM specimens' colour stability (p < 0.05), while polishing method had no significant influence (p > 0.05). The average ∆E values for specimens submerged in wine were considerably higher (p < 0.05) than those for the other solutions. SFRC CAD, Cerasmart 270, and Enamic displayed the highest ∆E values in wine (p < 0.05).Conclusions: The colour stability of tested SFRC CAD was comparable to other composite-based CAD/CAM materials, while IPS e.max exhibited the highest level of colour stability.","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-04-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11171852/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0