Influence of alcohol consumption on the levels of surfactant proteins SP-A and SP-D in blood in men and women in Novosibirsk

Abstract

The purpose of the study is to associate alcohol consumption with the level of surfactant proteins SP-A and SP-D in Novosibirsk population. Material and methods. 174 people (87 men and 87 women) aged from 45 to 69 were examined. The study participants were randomly selected. Alcohol consumption was assessed using the AUDIT test. The content of surfactant proteins SP-A and SP-D in blood serum was determined by enzyme immunoassay using standard ELISA test sets. Statistical analysis was performed using parametric and nonparametric methods of descriptive statistics, correlation analysis was performed using Spearman criterion, and multivariate analysis was performed using binary logistic regression. ROC analysis was used to evaluate the diagnostic significance of the variables and to determine sensitivity and specificity. The critical level of significance for the null hypothesis (p) was accepted to be 0.05 in all statistical analysis procedures. Results. A typical single dose of alcohol consumed of 7–9 alcohol units (TSDAC) was inversely associated with SP-A content (r = –0.154, p = 0.043) in a general sample of men and women aged 45–59 years in Novosibirsk. By means of ROC analysis, SP-A was found to be a marker (area under the ROC curve equals 71.2 %) of alcohol intake in TSDAC. With a SP-A level not more than 921 pg/ml, its sensitivity to the determination of alcohol consumption in TSDAC is 68.7 %, the specificity is 87.5 %. Regression analysis showed that the variable “TSDAC” directly affects the presence of SP-A in a concentration not preceding 921 pg/ml (Exp (B) = 13.0; 95 % CI = 1.5; 111.0, p = 0.019) regardless of age and sex. In the general sample of men and women in Novosibirsk aged 45–69 years, as well as in men, inverse associations of Sp-D content with the presence of >0 points were found in the answer to the AUDIT test question “How often during the last year have you not done something that was expected of you?”. It was determined that women had a higher level of SP-A in the blood than men. Perhaps this is due to the fact that women smoked less often than men (10.3 and 23.0 %, respectively, p = 0.020). So, it was established that a high TSDAC is an independent factor directly influencing the presence of SP-A level in the blood not exceeding 921 pg/ml for men and women aged 45–69 years in Novosibirsk.