Comparative chromatographic analysis of phenolic compounds of Liquidambar orientalis plant cultivated under in vitro salt stress

Abstract

In this study, the effects of boron salt stress on in vitro cultivated Liquidambar orientalis (L. orientalis), a relict-endemic plant species, and the resulting changes in its phenolic appearance were investigated. Salt stress can cause negative impact on plant growth and production, especially in species with low salinity and drought tolerance, affecting metabolite expression and somaclonal execution. To evaluate the effects of different boron salts on meristem regeneration and progression, clonal in vitro L. orientalis meristems were exposed to boric acid, sodium perborate, sodium metaborate, and disodium octaborate salts. When compared with the control group examples where salt application was not performed, the highest regeneration percentage was determined to be 100% with the application of 1 mg/L disodium octaborate. In terms of the shoot formation capacity index, it was determined to be 5 mg/L. With a value of 4.94, the application of sodium perborate yielded the best result. In L. orientalis plants, the greatest change in phenolic compounds due to boron salt applications was observed in the concentration of Quercetin with the sodium perborate salt application at 1 mg/L concentration.