A Study on the Effect of Non-thermal Plasma on Macrophage Phenotype Modulation

IF 2.6 3区物理与天体物理 Q3 ENGINEERING, CHEMICAL Plasma Chemistry and Plasma Processing Pub Date : 2023-10-29 DOI:10.1007/s11090-023-10414-y

Takia Sharfuddin, Diego Jacho, Dalton Mitchey, Eda Yildirim-Ayan, Halim Ayan

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Abstract

Non-thermal plasma has gained significant popularity in the field of biology due to its ability to generate reactive oxygen and nitrogen species, which can alter gene expression within cells. Plasma treatment has been used to address various medical issues, such as cancer treatment, blood clotting, and bacterial sterilization. In our study, we investigated the effect of non-thermal plasma treatment on monocyte-derived macrophages (U937) in vitro. To create an in vivo-like environment, the cells were embedded in a collagen matrix and treated with a di-electric barrier non-thermal plasma system using a combination of air and helium flow. Our results showed that plasma in air might modulate macrophages through changes in gene expression. Further experiments revealed the presence of reactive species responsible for these changes. By evaluating cell viability and gene expression, we determined that 120 s of plasma treatment in air is optimal for Fibroblast cells within collagen. Our study suggests that non-thermal plasma in air can modulate monocyte-derived macrophages embedded in collagen.

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非热等离子体对巨噬细胞表型调节效果的研究

非热等离子体能够产生活性氧和氮物种，从而改变细胞内的基因表达，因此在生物学领域大受欢迎。等离子体处理已被用于解决各种医学问题，如癌症治疗、血液凝固和细菌杀菌。在我们的研究中，我们调查了非热等离子体处理对体外单核巨噬细胞（U937）的影响。为了创造类似活体的环境，我们将细胞包埋在胶原基质中，并使用空气流和氦气流相结合的二电屏障非热等离子体系统进行处理。我们的结果表明，空气中的等离子体可能会通过改变基因表达来调节巨噬细胞。进一步的实验揭示了导致这些变化的活性物种的存在。通过评估细胞活力和基因表达，我们确定在空气中进行 120 秒的等离子处理对胶原蛋白中的成纤维细胞来说是最佳的。我们的研究表明，空气中的非热等离子体可以调节嵌入胶原蛋白中的单核巨噬细胞。

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来源期刊

Plasma Chemistry and Plasma Processing 工程技术-工程：化工

CiteScore

5.90

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Publishing original papers on fundamental and applied research in plasma chemistry and plasma processing, the scope of this journal includes processing plasmas ranging from non-thermal plasmas to thermal plasmas, and fundamental plasma studies as well as studies of specific plasma applications. Such applications include but are not limited to plasma catalysis, environmental processing including treatment of liquids and gases, biological applications of plasmas including plasma medicine and agriculture, surface modification and deposition, powder and nanostructure synthesis, energy applications including plasma combustion and reforming, resource recovery, coupling of plasmas and electrochemistry, and plasma etching. Studies of chemical kinetics in plasmas, and the interactions of plasmas with surfaces are also solicited. It is essential that submissions include substantial consideration of the role of the plasma, for example, the relevant plasma chemistry, plasma physics or plasma–surface interactions; manuscripts that consider solely the properties of materials or substances processed using a plasma are not within the journal’s scope.