Molecular characterization of multidrug resistant (MDR) clinical isolates of Pseudomonas aeruginosa from Nsukka, South Eastern Nigeria

IF 0.8 4区医学 Q3 MEDICINE, GENERAL & INTERNAL African Health Sciences Pub Date : 2023-10-11 DOI:10.4314/ahs.v23i3.57

Martina C Agbo, Kenneth O Ugwu, Boniface N Ukwah, Ifeoma M Ezeonu

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Abstract

Background: P. aeruginosa is an important nosocomial pathogen with increasing resistance to antibiotics. Objective: This studywas performed to evaluate the genetic relatedness of MDR clinical isolates of P. aeruginosa. Method: A total of 1000 samples were analysed in the study. Antibiotic resistance profiles of the isolates were determined usingKirby Bauer disk diffusion method. Polymerase chain reaction (PCR) and sequencing were simultaneously used to detect theconsensus region of 16S rRNA. Genetic relatedness of the isolates was determined using restriction patterns from ALU 1 digestand random amplified polymorphic DNA. Results: Out of the 192 P. aeruginosa isolates recovered, 136 (78.83%) were multidrug resistant. Sequence analysis of the confirmedisolates (80.68%) revealed that all the isolates shared homology with each other and also showed sequence similarity toknown strains of P. aeruginosa (ATCC 27853; KT 315654; KU 321274 and KT894767). The PCR-Restriction fragment lengthpolymorphism (PCR-RFLP) analysis revealed that there was a lot of genetic relatedness among the isolates. The RFLP fingerprinting technique detected seven distinct RFLP types among the isolates. Conclusions: Thus, study shows that there is high prevalence of MDRPA and high degree of genetic relatedness among theMDRPA isolates circulating in Nsukka area. Keywords: MDRPA; PCR-RFLP; RAPD; Sequencing; 16S rRNA gene.

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尼日利亚东南部Nsukka多药耐药铜绿假单胞菌临床分离株的分子特征

背景:铜绿假单胞菌(P. aeruginosa)是一种重要的院内病原菌，对抗生素的耐药性日益增强。目的:评价耐多药铜绿假单胞菌临床分离株的遗传相关性。方法:对1000份样本进行分析。采用kirby Bauer纸片扩散法测定菌株的耐药谱。采用聚合酶链反应(PCR)和测序同时检测16S rRNA的一致区。利用ALU 1消化酶和随机扩增多态性DNA的限制性内切模式来确定分离物的遗传亲缘关系。结果:回收的192株铜绿假单胞菌中，有136株(78.83%)耐多药。序列分析表明，所有分离株均具有同源性，且序列与已知铜绿假单胞菌(P. aeruginosa, ATCC 27853;KT 315654;kt321274和KT894767)。聚合酶链反应-限制性内切片段长度多态性(PCR-RFLP)分析表明，分离株间存在较强的遗传亲缘关系。RFLP指纹图谱技术检测出7种不同的RFLP类型。结论:研究表明，Nsukka地区流行的MDRPA分离株具有较高的流行率和高度的遗传亲缘性。关键词:MDRPA;PCR-RFLP;RAPD;测序;16S rRNA基因。

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来源期刊

African Health Sciences MEDICINE, GENERAL & INTERNAL-

CiteScore

2.30

自引率

0.00%

发文量

179

审稿时长

>12 weeks

期刊介绍： The African Health Sciences is an internationally refereed journal publishing original articles on research, clinical practice, public health, policy, planning, implementation and evaluation, in the health and related sciences relevant to Africa and the tropics. Its objectives are to: Advocate for and promote the growth of reading culture in sub Saharan Africa; Provide a high quality journal in which health and policy and other researchers and practitioners in the region can and world wide, can publish their work; Promote relevant health system research and publication in the region including alternative means of health care financing, the burden of and solution of health problems in marginalized urban and rural communities amongst the displaced and others affected by conflict; Promote research and the systematic collection and collation and publication of data on diseases and conditions of equity and influence; Promote development of evidence-based policies and guidelines for clinical, public health and other practitioners. African Health Sciences acknowledges support provided by the African Health Journals Partnership Project that is funded by the US National Institutes of Health (through the National Library of Medicine and the Fogarty International Center) and facilitated by the Council of Science Editors.