Comparison of Temporary Immersion Bioreactor (SETISTM) and Classical Solid Culture in Micropropagation of ‘Grand Naine’ (Musa spp.) Banana Cultivar

Abstract

Banana is an important nutritional source for millions of people because it contains protein, carbohydrates, vitamins and minerals. Therefore, the need for banana production is increasing day by day. Various methods are being developed to meet this need. Among these methods, biotechnological applications are becoming more prevalent over time. In micropropagation of in vitro plantlets, which forms the basis of banana production and is one of the most important stages, the methods used and banana varieties must be selected correctly. Therefore, it is very important to decide which protocol is suitable for which variety. In TIBs, in vitro micropropagation and rooting were compared with solid culture by adjusting the frequency of immersion of in vitro plantlets into the nutrient medium and the contact time of nutrients to the plantlets in the immersion medium. It was determined that the TIB application was more successful than the solid medium experiment in many aspects, especially the micropropagation rate (4.20) and rooting rate (84%).