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Effects of Supplementary Pollination on Macadamia Nut Set, Retention and Yield in Murang’a County, Kenya 补充授粉对肯尼亚穆郎阿县夏威夷果的结实率、保留率和产量的影响
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p63
Nicholas I. Njue, J. Muthomi, G. Chemining’wa, J. Nderitu, James J. Odanga
Macadamia is a promising prime dessert nut with the potential of alleviating poverty and enhancing food security in Kenya. Nut set and subsequent development of nuts to maturity is dependent on pollination which is mediated by animals, and honey bees are the dominant macadamia flower visitors. However, macadamia is pollen deficit as not all flowers set develop to mature nuts, thus supplemental pollination results to better nut yields. There is limited information in Kenya among the smallholder macadamia growers on the importance of supplementing pollination to enhance nut yields. This study was conducted at Kandara Macadamia Research Centre and in a smallholder macadamia farm located 15 km from the research centre, which was purposively selected from July 2021 to May 2023. Assessment of the influence of pollinators and supplementing pollination to nut set, retention and final yields was done. Honey bee, (Apis mellifera L.) colonies were also introduced at the Macadamia Research Centre to enhance pollination and mitigate deficits. Racemes were bagged to exclude pollinators, others left open to unlimited pollinator access while others received supplemental hand pollination. The results demonstrate that macadamia is pollen limited and pollination is highly reliant on insect pollinators. There were significant differences (p < 0.001) among the bagged (pollinator exclusion), open and hand pollinated racemes in both farms. The initial nut set and retention was increased significantly (21.54%) in hand pollinated racemes compared to those that were left to open pollination (unlimited pollinator visits) depicting pollination deficit in farm B. Introduction of supplementary honey bee colonies at the Kandara Macadamia Research Centre, resulted to higher nut set, retention, nut-in-shell and kernels. Macadamia growers are encouraged to introduce honey bee colonies in their farms to improve yields and also address pollination deficits.
澳洲坚果是一种前景广阔的优质甜点坚果,具有减轻肯尼亚贫困和提高粮食安全的潜力。坚果的着生和随后的成熟取决于动物的授粉,蜜蜂是澳洲坚果花的主要访客。然而,澳洲坚果缺乏花粉,因为并非所有花朵都能发育成成熟坚果,因此补充授粉可提高坚果产量。在肯尼亚,关于补充授粉对提高坚果产量的重要性,小农澳洲坚果种植者的信息很有限。这项研究是在坎达拉澳洲坚果研究中心和距离研究中心 15 公里处的一个小农澳洲坚果农场进行的,研究时间为 2021 年 7 月至 2023 年 5 月。研究人员评估了授粉者和辅助授粉对坚果坐果率、保留率和最终产量的影响。澳洲坚果研究中心还引进了蜜蜂(Apis mellifera L.)蜂群,以加强授粉和缓解赤字。将总状花序装入袋中以排除授粉者,其他花序则敞开以让授粉者无限制地进入,而其他花序则接受人工授粉的补充。结果表明,澳洲坚果的花粉有限,授粉高度依赖昆虫授粉者。在两个农场中,袋装(排除授粉者)、开放和人工授粉的总状花序之间存在明显差异(p < 0.001)。与开放授粉(授粉者访问次数不限)的葡萄相比,人工授粉葡萄的最初坚果坐果率和保留率显著提高(21.54%),说明 B 农场授粉不足。鼓励澳洲坚果种植者在其农场引进蜜蜂群,以提高产量并解决授粉不足的问题。
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引用次数: 0
Research on the Factors Influencing the Security of Grain Supply Capacity in Sichuan Province Based on PLS Structural Equation Modelling 基于 PLS 结构方程模型的四川省粮食供给能力保障影响因素研究
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p22
Qian Liu, Yingying Dai, Jiahui Tang, Haoyue Gan, Yinzhou Zhao
Sichuan Province, distinguished as one of the top performers among China’s 13 main grain-producing provinces, holds unique advantages in the western region. The continuous increase in grain production lays a solid groundwork for upholding the country’s food security. Grounded in five dimensions—social, economic, technological, resource, and environmental—this article establishes a security evaluation system for the grain supply capacity of Sichuan Province, incorporating 14 specific indicators, and utilizes a PLS structural equation model to investigate the diverse factors influencing the security of Sichuan Province’s grain supply capacity. Findings reveal that social and technological advancements directly negatively affect the security of the grain supply capacity, while economic growth, environmental progress, and resource enhancement directly positively influence grain supply capacity security. It also corroborates that a sustainable grain supply capacity necessitates the harmonious development of these five facets, each being essential. As a result, strategies to safeguard the security of Sichuan Province’s grain supply capacity are put forward, aiming to offer decision-making references for strengthening and elevating Sichuan’s grain supply capability and constructing an advanced “Heavenly Granary”.
四川省在全国 13 个粮食主产省中名列前茅,在西部地区具有得天独厚的优势。粮食产量的持续增长为维护国家粮食安全奠定了坚实基础。本文从社会、经济、科技、资源和环境五个维度出发,建立了包含 14 个具体指标的四川省粮食供给能力安全评价体系,并利用 PLS 结构方程模型研究了影响四川省粮食供给能力安全的多种因素。研究结果表明,社会和科技进步直接对粮食供给能力安全产生负面影响,而经济增长、环境进步和资源改善直接对粮食供给能力安全产生正面影响。研究还证实,可持续的粮食供应能力需要这五个方面的协调发展,缺一不可。由此,提出了保障四川省粮食供给能力安全的策略,旨在为加强和提升四川省粮食供给能力、建设先进的 "天府粮仓 "提供决策参考。
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引用次数: 0
Effects of Intercropping on Maize and Soybean Yield Performance, Land Equivalent Ratio, and Maize Leaf Area in Conservation Agriculture 保护性耕作中间作对玉米和大豆产量表现、土地当量比和玉米叶面积的影响
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p37
Otim Godfrey Anyoni, Tumwebaze Susan, Ekwangu Joseph, Mudde Barnabas, Obia Alfred
Maize-soybean intercropping systems as a conservation farming practice are receiving increased focus from the scientific community. This is because of the advantages of intercropping, especially nutrient benefits through cereal-legume interactions, alternative sustainable methods to manage biotic stress (pests, diseases, weeds), and crop failure risk management due to erratic weather. In addition, smallholders in developing countries commonly use intercropping to produce crops. In Uganda, 40% of smallholder farmers are practicing intercropping yet no adequate location-specific information is available to inform their practice. Farmers who adopted conservation farming practices did not mulch their maize due to drudgery associated with collecting mulch. This study evaluated the effect of two tillage methods (T1 = Conventional tillage using ox drawn mould board plough, T2 = Minimum tillage using ox drawn ripper) and five soil cover practices (SC1 = Mulched Maize, SC2 = Control no mulch, SC3 = Two rows of soybean in between one row of maize, SC4 = One row of soybean in between one row of maize, SC5 = Sole soybean) on maize and soybean yield performance. The trials were established for 4 rainy seasons on a sandy loam ferrosol at National Agricultural Research Organization Institute in Lira, Uganda. Soil cover practice had a significant effect on maize and soybean crop Yield. Mulching significantly increased maize yield and LAI. The LER for both intercropping partterns were above 1.2. Tillage methods were not significantly different in determining crop performance. The practice of minimum tillage should also be adopted because it enhances the positive effects of soil cover (intercropping). We recommend farmers to adopt the intercropping pattern of one row of soybean in between maize row spaced at 75 × 30 cm for better LER, and crop performance. This intercropping pattern maximizes on available resources to deliver better output in conservation farming. Maize crop generally performed better during first season as compared to second season. We recommend farmers to utilize the first rains as the main maize production season.
作为一种保护性耕作法,玉米-大豆间作系统正受到科学界越来越多的关注。这是因为间作的优势,特别是通过谷物与豆类的相互作用产生的养分效益、管理生物压力(虫害、疾病、杂草)的替代性可持续方法,以及因天气变化无常而导致的作物歉收风险管理。此外,发展中国家的小农户通常使用间作方法生产作物。在乌干达,40% 的小农户采用间作方法,但没有足够的针对具体地点的信息来指导他们的做法。采用保护性耕作法的农民不会对玉米进行覆膜,因为收集覆膜很费力。本研究评估了两种耕作方法(T1 = 使用牛拉模板犁的传统耕作法,T2 = 使用牛拉开沟器的少耕法)和五种土壤覆盖方法(SC1 = 玉米覆膜,SC2 = 无覆膜对照,SC3 = 一行玉米间两行大豆,SC4 = 一行玉米间一行大豆,SC5 = 单一大豆)对玉米和大豆产量表现的影响。试验在乌干达里拉国家农业研究组织研究所的沙质壤土上进行,历时 4 个雨季。土壤覆盖对玉米和大豆作物产量有显著影响。地膜覆盖明显提高了玉米产量和LAI。两种间作模式的 LER 均高于 1.2。耕作方法在决定作物表现方面没有明显差异。由于最小耕作能增强土壤覆盖(间作)的积极效果,因此也应采用最小耕作。我们建议农民采用玉米行间种一行大豆的间作模式,间距为 75 × 30 厘米,以获得更好的 LER 和作物表现。这种间作模式能最大限度地利用现有资源,在保护性耕作中实现更好的产出。与第二季相比,玉米作物在第一季的表现通常更好。我们建议农民利用第一季降雨作为玉米生产的主要季节。
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引用次数: 0
Reviewer Acknowledgements for Journal of Agricultural Science, Vol. 16, No. 1 农业科学杂志》第 16 卷第 1 期审稿人致谢
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p90
A. Brown
Reviewer acknowledgements for Journal of Agricultural Science, Vol. 16, No. 1, 2024.
农业科学杂志》第 16 卷第 1 期审稿人致谢,2024 年。
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引用次数: 0
Physico-chemical and Nutritional Composition of Ten Sorghum (Sorghum bicolor L.) Grain Varieties as Potential Feed for Livestock 十种高粱(Sorghum bicolor L.)谷物品种作为牲畜潜在饲料的理化和营养成分
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p1
A. Yousuf, David Brown, Muzi Ginnidza, Abraha-Eyob Zaid, Edward Sismour
Sorghum grain (Sorghum bicolor (L.) Moench) is gaining importance amongst livestock producers and animal nutritionists. Newly developed sorghum varieties should be evaluated for their suitability in small ruminant ration. The aim of this research was to determine the physico-chemical characteristics of ten sorghum varieties as potential feed for small ruminants. There were no significant differences (P > 0.05) in DM, CP and CF contents of the ten sorghum varieties. The sorghum black had higher GE, NDF and ADF contents and ATxArg had higher oil and mineral contents. Similarly, sorghum varieties labelled black, red, R17049 and FZ2CND were characterized by high tannins, phenolic and redness contents. Marcia, R17231, RTx436 contained high levels of yellowness and lightness. Fatty acid profile also varies amongst the ten varieties. The results of this study demonstrated that physiochemical and nutritional properties of sorghum varied by varieties.
高粱谷物(Sorghum bicolor (L.) Moench)越来越受到畜牧生产者和动物营养学家的重视。应评估新开发的高粱品种是否适合作为小型反刍动物的饲料。本研究的目的是确定十个高粱品种作为小型反刍动物潜在饲料的物理化学特性。十个高粱品种的 DM、CP 和 CF 含量差异不大(P > 0.05)。黑高粱的 GE、NDF 和 ADF 含量较高,ATxArg 的油脂和矿物质含量较高。同样,黑高粱、红高粱、R17049 和 FZ2CND 的单宁、酚和红色素含量也较高。Marcia、R17231 和 RTx436 的黄度和亮度较高。十个品种的脂肪酸含量也各不相同。研究结果表明,不同品种高粱的理化和营养特性各不相同。
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引用次数: 0
Camel Herd Structure and Performance in Isiolo and Marsabit Counties, Kenya 肯尼亚伊西奥洛县和马萨比特县的驼群结构和表现
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p75
Florence K. Thiakunu, B. Njehia, P. Nguhiu, J. Arimi
Urbanization and improved middle-level class income have caused an increase in demand for animal products and allowed economic growth in developing countries. Arid and semi-arid lands (ASALs), which support high livestock populations have an opportunity to contribute to the economy. However, their potential may not be realized fully due to the effects of climate change. This study aimed to establish camel herd structure and performance in Isiolo and Marsabit Counties, Kenya. Focus Group Discussions (FGDs) and a cross-sectional study were conducted from July to August 2022 in each County. Data on the herd size and composition (the number of age and sex categories) was collected from 388 households through structured questionnaires. The pregnancy test was done on seventeen camels four months after the mating season by tail cocking and progesterone assay method. Analysis was done using descriptive, correlations, and linear regression statistics at a 0.05 significance level. There were more female camels over 4 years in Isiolo (above 12%) than in Marsabit (below 10%). In Isiolo, milk yield was above 20 and 10 liters per day in wet and dry seasons respectively, while Marsabit production was below 10 liters in both seasons. Milk production coefficients were significant for the females above four years (p < 0.05). There were more households in Marsabit (63%) having a calving interval of 24 months and below than in Isiolo (50%). The tail cocking method did not correlate with the progesterone assay method in pregnancy diagnosis. The study concludes that, in Isiolo County, pastoralists are doing camel rearing with the objective of milk production unlike in Marsabit County. The recommendation is that pastoralists should be encouraged to adjust age and sex categories to increase milk production. Further, they need to apply an accurate cost-effective pregnancy testing method.
城市化和中产阶级收入的提高导致了对动物产品需求的增加,并推动了发展中国家的经济增长。干旱和半干旱地区(ASALs)养育着大量牲畜,有机会为经济做出贡献。然而,由于气候变化的影响,它们的潜力可能无法充分发挥。本研究旨在确定肯尼亚伊西奥洛县和马萨比特县的骆驼群结构和表现。2022 年 7 月至 8 月,在两个县分别开展了焦点小组讨论(FGD)和横断面研究。通过结构化问卷从 388 个家庭收集了有关牛群规模和组成(年龄和性别类别数量)的数据。在交配季节结束四个月后,对 17 头骆驼进行了妊娠测试,测试方法是翘尾和黄体酮检测法。在 0.05 的显著性水平下,使用描述性、相关性和线性回归统计进行了分析。伊西奥洛 4 年内的母骆驼数量(12% 以上)多于马萨比特(10% 以下)。伊西奥洛在雨季和旱季的日产奶量分别高于 20 升和 10 升,而马萨比特在这两个季节的日产奶量均低于 10 升。四岁以上女性的产奶量系数显著(P < 0.05)。产犊间隔在 24 个月及以下的马萨比特家庭(63%)多于伊西奥洛家庭(50%)。在妊娠诊断方面,翘尾法与黄体酮检测法没有相关性。研究得出的结论是,与马萨比特县不同,伊西奥洛县的牧民饲养骆驼的目的是为了产奶。建议应鼓励牧民调整骆驼的年龄和性别,以提高产奶量。此外,他们还需要采用成本效益高的准确妊娠检测方法。
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引用次数: 0
Characterisation and Mapping of Soils in Major Coffee Growing Regions of Uganda 乌干达主要咖啡种植区土壤特性分析与绘图
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p49
Gerald Kyalo, P.C. Apunyo, M. Mwanjalolo, Charles Kizza Luswata, Ronald Kawooya, E. I. Niyibigira
There is limited information on the soil nutrient status, site specific fertilizer and agronomic recommendations for coffee in Uganda hence limiting its production and productivity. Therefore, this study was undertaken to characterize the soils under coffee farms and provide fertilizer and land management recommendations for coffee farming in selected districts in Uganda. 717 soil samples were collected from 45 coffee growing districts that were purposively selected to represent the major coffee growing regions of Eastern, Northern, Western, Mid-west and West Nile. 35 districts were sampled from Robusta coffee growing areas and 10 districts from Arabica coffee growing areas. Parameters considered in the laboratory analysis included; pH, total organic carbon, total nitrogen, available P, exchangeable Calcium (Ca), Sodium (Na), Magnesium (Mg), and Potassium (K) and micro elements: Iron (Fe), Zinc (Zn), Copper (Cu), Manganese (Mn) and Boron, soil texture, bulk density and hydraulic conductivity. Soil nutrient levels distribution maps for Robusta and Arabica coffee growing regions were generated in ArcGIS for the entire country. The soil chemical and physical properties were subjected to analysis of variance using Genstat 14th edition. The soil mapping results showed that, the overall average soil macro-nutrients concentrations were significantly different across regions (p < 0.05) with Eastern having the highest levels of macro elements (CEC of 19.28 meq/100 g, Base Saturation (BS) of 43.40%, pH of 5.78, N of 0.20%, K of 1.64 meq/100 g, P of 107.68 mg/kg and OM of 3.31%) followed by Western region. No significant difference (p > 0.05) was recorded for the micronutrients across the different regions, except Zn (p < 0.05). This study showed that Phosphorus and Potassium were generally moderate to high levels in most of the regions sampled while Nitrogen and organic matter were moderate to low. Results from this study provide a general picture of the nutrient status across all coffee growing regions in Uganda and highlight the required modifications for increased production and productivity.
有关乌干达咖啡土壤养分状况、具体施肥地点和农艺建议的信息有限,因此限制了其产量和生产率。因此,本研究旨在分析咖啡农场土壤的特征,并为乌干达选定地区的咖啡种植提供肥料和土地管理建议。从 45 个咖啡种植区收集了 717 份土壤样本,这些样本是有目的性地挑选出来的,代表了东部、北部、西部、中西部和西尼罗河等主要咖啡种植区。其中 35 个采样区来自罗布斯塔咖啡种植区,10 个采样区来自阿拉比卡咖啡种植区。实验室分析中考虑的参数包括:pH 值、总有机碳、总氮、可利用磷、可交换钙(Ca)、钠(Na)、镁(Mg)和钾(K)以及微量元素:铁(Fe)、锌(Zn)、铜(Cu)、锰(Mn)和硼、土壤质地、容重和导水率。在 ArcGIS 中生成了全国罗布斯塔和阿拉比卡咖啡种植区的土壤养分含量分布图。使用 Genstat 第 14 版对土壤化学和物理特性进行了方差分析。土壤制图结果表明,各地区土壤宏量营养元素的总体平均浓度存在显著差异(p < 0.05),东部地区的宏量元素含量最高(CEC 为 19.28 meq/100 g,碱饱和度 (BS) 为 43.40%,pH 为 5.78,氮含量为 0.20%,钾含量为 1.64 meq/100 g,磷含量为 107.68 mg/kg,OM 为 3.31%),其次是西部地区。除了锌(p < 0.05)外,不同地区的微量营养元素没有明显差异(p > 0.05)。这项研究表明,在大多数采样地区,磷和钾的含量普遍处于中等到较高水平,而氮和有机质的含量则处于中等到较低水平。这项研究的结果提供了乌干达所有咖啡种植区养分状况的总体情况,并强调了提高产量和生产率所需的调整措施。
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引用次数: 0
Sensitivity of Azuki Bean and Control of Multiple Herbicide-Resistant Canada Fleabane With Saflufenacil Herbicide Mixtures 阿月浑子的敏感性和使用 Saflufenacil 除草剂混合物控制多种抗除草剂的加拿大金花菜
IF 2 4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-12-15 DOI: 10.5539/jas.v16n1p13
N. Soltani, C. Shropshire, P. Sikkema
During 2021 and 2022, four experiments were conducted to ascertain the sensitivity of azuki bean to saflufenacil herbicide mixtures, and five experiments were conducted to determine the control of multiple herbicide-resistant (MHR) Canada fleabane with various saflufenacil herbicide mixtures applied preplant (PP) in soybean at various locations in southwestern Ontario, Canada. At 1, 2, 4, and 8 weeks after emergence (WAE), glyphosate + saflufenacil caused 2-5% azuki bean injury. The addition of metribuzin, bromoxynil, halauxifen-methyl, or 2,4-D ester caused 2-7%, 2-4%, 4-9%, and 2-4% azuki bean injury, respectively. Glyphosate + saflufenacil + bromoxynil plus either metribuzin, halauxifen-methyl, or 2,4-D ester caused 3-7%, 5-11%, and 3-6% azuki bean injury, respectively. Saflufenacil mixtures evaluated had no adverse effect on azuki bean stand, biomass m-1, biomass plant-1, height, seed moisture content, or yield. At 4 and 8 weeks after application (WAA), glyphosate + saflufenacil control MHR Canada fleabane 93 and 87%, respectively; there was no improvement in MHR Canada fleabane control with the glyphosate + saflufenacil mixtures evaluated. At 8 WAA, saflufenacil herbicide mixtures evaluated reduced MHR Canada fleabane density 43-95% and biomass 47-96%; differences were not statistically significant. MHR Canada fleabane interference reduced soybean yield 50%; however, reduced MHR Canada fleabane interference with all glyphosate + saflufenacil mixtures evaluated resulted in soybean yield that was similar to the weed-free control. This study concludes that saflufenacil herbicide mixtures evaluated have the potential to be used for the control MHR Canada fleabane in azuki bean.
2021 年和 2022 年期间,在加拿大安大略省西南部不同地点进行了四次试验,以确定茨木豆对氟虫腈除草剂混合物的敏感性,并进行了五次试验,以确定在大豆播种前(PP)施用各种氟虫腈除草剂混合物对多种抗除草剂(MHR)加拿大飞燕草的控制效果。在出苗后 1、2、4 和 8 周(WAE),草甘膦 + 氟虫腈会造成 2-5% 的红豆伤害。添加灭幼脲、溴虫腈、甲基噻虫嗪或 2,4-D 酯分别会造成 2-7%、2-4%、4-9% 和 2-4% 的茨木豆损伤。草甘膦+氟虫腈+溴虫腈+甲基立枯磷、甲基噻唑膦或 2,4-D 酯分别造成 3-7%、5-11% 和 3-6% 的茨木豆受害。所评估的 Saflufenacil 混合物对红豆的株高、生物量 m-1、生物量 plant-1、株高、种子含水量或产量均无不利影响。施药后 4 周和 8 周(WAA),草甘膦 + 氟虫腈对 MHR 加拿大飞燕草的控制率分别为 93% 和 87%;草甘膦 + 氟虫腈混合物对 MHR 加拿大飞燕草的控制没有改善。在 8 WAA 时,所评估的 saflufenacil 除草剂混合物可使 MHR 加拿大飞蓬密度减少 43-95%,生物量减少 47-96%;差异无统计学意义。MHR 加拿大飞蓬的干扰使大豆产量降低了 50%;然而,使用所有草甘膦+氟虫腈混合物降低 MHR 加拿大飞蓬的干扰后,大豆产量与无杂草对照相似。本研究的结论是,所评估的氟虫腈除草剂混合物有可能用于控制加拿大MHR飞蓬在红豆中的生长。
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引用次数: 0
Microbial Biotransformation of Agro-waste Into Biovanillin as Flavor: A Process Optimization by Response Surface Methodology 利用响应面法优化农业废弃物转化为香兰素的过程
4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-11-15 DOI: 10.5539/jas.v15n12p74
M. Sujatha, R. Jaya Madhuri, K. Thagaraju
Vanillin is a flavour that is commonly employed in the food industry, but plant sources can yield a small amount of this molecule. Fermentative production of biovanillin by optimizing different process parameters such as specific pH, temperature, incubation time, carbon source, nitrogen source, ferulic acid substrate, and by agro wastes are beneficial in terms of fermentation economics and scale up of the process response surface methodology for an ideal production of biovanillin yield. Hence the present research emphasizes on optimization of fermentation conditions for employing Bacillus licheniformis MSJM5 isolate. As per the experimental results biovanillin production yield was enhanced at neutral pH, mesophilic temperature, in an incubation of 48 hrs, under revolving conditions, in the presence of glucose as carbon source soya bean as nitrogen source ferulic acid 0.3 mg concentration and ground nut oil cake, an agro waste material has stimulated the formation of biovanillin, 326-834 mg/ml. Further stastical analysis by RSM the yield of biovanillin was 1.89 mg/ml. Based on the morphological cultural and biochemical the positive strain MSJM5 is identified as Bacillus licheniformis with 98% homology and accession number ON413745. Strain improvement studies have reveated that UV light and Et Br use reduce the survival capacity of Bacillus licheniformis.
香草素是食品工业中常用的一种香料,但植物来源可以产生少量的这种分子。通过优化不同的工艺参数,如特定pH值、温度、培养时间、碳源、氮源、阿魏酸底物和农业废弃物,发酵生产生物香兰素,有利于发酵经济学和扩大工艺响应面方法的规模,以达到理想的生物香兰素产量。因此,本研究的重点是地衣芽孢杆菌MSJM5分离物发酵条件的优化。实验结果表明,在中性pH、中温条件下,培养48小时,在循环条件下,以葡萄糖为碳源、大豆为氮源、阿魏酸0.3 mg浓度和花生油饼等农业废弃物刺激生物香兰素的生成,326 ~ 834 mg/ml。经RSM进一步统计分析,生物香兰素得率为1.89 mg/ml。经形态培养和生化鉴定,阳性菌株MSJM5为地衣芽孢杆菌,同源性98%,菌株编号为ON413745。菌株改良研究表明,紫外光和溴氰酸盐的使用降低了地衣芽孢杆菌的存活能力。
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引用次数: 0
Comparison of Temporary Immersion Bioreactor (SETISTM) and Classical Solid Culture in Micropropagation of ‘Grand Naine’ (Musa spp.) Banana Cultivar 暂浸式生物反应器(setstm)与经典固体培养在香蕉品种“大奈恩”(Musa spp.)微繁中的比较
4区 农林科学 Q2 AGRICULTURE, MULTIDISCIPLINARY Pub Date : 2023-11-15 DOI: 10.5539/jas.v15n12p51
Taner Bozkurt, Sezen İnan, İjlal Dündar
Banana is an important nutritional source for millions of people because it contains protein, carbohydrates, vitamins and minerals. Therefore, the need for banana production is increasing day by day. Various methods are being developed to meet this need. Among these methods, biotechnological applications are becoming more prevalent over time. In micropropagation of in vitro plantlets, which forms the basis of banana production and is one of the most important stages, the methods used and banana varieties must be selected correctly. Therefore, it is very important to decide which protocol is suitable for which variety. In TIBs, in vitro micropropagation and rooting were compared with solid culture by adjusting the frequency of immersion of in vitro plantlets into the nutrient medium and the contact time of nutrients to the plantlets in the immersion medium. It was determined that the TIB application was more successful than the solid medium experiment in many aspects, especially the micropropagation rate (4.20) and rooting rate (84%).
香蕉是数百万人的重要营养来源,因为它含有蛋白质、碳水化合物、维生素和矿物质。因此,对香蕉生产的需求日益增加。为了满足这一需要,正在开发各种方法。在这些方法中,随着时间的推移,生物技术的应用变得越来越普遍。离体苗的微繁是香蕉生产的基础和重要阶段之一,必须正确选择方法和品种。因此,决定哪种协议适用于哪种品种是非常重要的。在TIBs中,通过调整离体苗浸泡在营养培养基中的频率和营养物质与试管苗的接触时间,比较了试管苗的离体增殖和生根情况。结果表明,TIB在许多方面都比固体培养基试验成功,特别是微繁率(4.20%)和生根率(84%)。
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Journal of Agricultural Science
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