A study on bitter gourd (Momordica charantia L.) callogenesis optimization based on hormone balance and explant types

Tugce OZSAN KILIC, Ahmet Naci ONUS
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Abstract

Bitter gourd known as Momordica charantia L. is an important summer vegetable, belongs to the Cucurbitaceae family, grown in tropical areas in the world. Due to its fruits being rich in vitamins, minerals and good dietary fibers, the bitter gourd has many health-protective and pharmacological properties. The goal of the current study was to comparatively assess the indirect regeneration capability using in vivo leaf, internodal, and petiole explants of bitter gourd. To serve the purpose Murashige and Skoog (MS) media augmented with various concentrations of auxin and cytokinin for callus formation potent. The study was conducted by evaluating the selection of various auxin and cytokinin concentrations and combinations in MS basic medium. The rate of explant development decreased as cytokinin concentration increased. Although the lowest cytokinin concentration utilized in this study (1.0 mg L-1 BAP), it had a beneficial impact on explant development. When compared to the auxin NAA, the auxins IAA and 2,4-D were more beneficial on calli weights. It has been well proven that calli cannot be produced without the presence and balance of plant growth regulators. Experimental results demonstrated that callogenesis of bitter gourd from different explants might be successfully and effectively utilized in cell suspension cultures, genetic transformation, and callogenesis may also be adaptable to other species that are closely related.
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基于激素平衡和外植体类型优化苦瓜(Momordica charantia L.)骨形成的研究
苦瓜(Momordica charantia L.)是一种重要的夏季蔬菜,属于葫芦科,生长在世界热带地区。由于其果实富含维生素、矿物质和良好的膳食纤维,苦瓜具有许多保健和药理作用。本研究的目的是比较评估苦瓜叶片、节间和叶柄外植体的间接再生能力。在Murashige和Skoog (MS)培养基中添加不同浓度的生长素和细胞分裂素对愈伤组织的形成有促进作用。本研究通过对MS基本培养基中不同生长素和细胞分裂素浓度和组合的选择进行评价。随着细胞分裂素浓度的增加,外植体发育速率降低。虽然本研究中使用的细胞分裂素浓度最低(1.0 mg L-1 BAP),但它对外植体发育有有益的影响。与生长素NAA相比,生长素IAA和2,4- d对愈伤组织重量的影响更大。研究表明,愈伤组织的产生离不开植物生长调节剂的存在和平衡。实验结果表明,不同外植体的苦瓜愈伤组织可以成功有效地应用于细胞悬浮培养和遗传转化中,也可以应用于其他近缘种的苦瓜愈伤组织。
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8 weeks
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