{"title":"Photoswitchable vibrational nanoscopy with sub-100-nm optical resolution","authors":"Jianpeng Ao, Xiaofeng Fang, Liyang Ma, Zhijie Liu, Simin Wu, Changfeng Wu, Minbiao Ji","doi":"10.1117/1.ap.5.6.066001","DOIUrl":null,"url":null,"abstract":"Stimulated Raman scattering (SRS) microscopy has shown superior chemical resolution due to the much narrower vibrational spectral bandwidth than its fluorescence counterpart. However, breaking the diffraction-limited spatial resolution of SRS imaging is much more challenging because of the intrinsically weak scattering cross section and inert/stable nature of molecular bond vibrations. We report superresolution SRS (SR-SRS) nanoscopy based on reversible-switchable vibrational photochromic probes integrated with point spread function engineering strategy. By introducing a Gaussian-shaped ultraviolet excitation beam and a donut-shaped visible depletion beam in addition to the pump and Stokes beams, SR-SRS could reach sub-100 nm resolution on photoswitchable nanoparticles (NPs). Furthermore, NP-treated live cell imaging was demonstrated with resolution improvement by a factor of ∼4. Our proof-of-principle work provides the potential for SR vibrational imaging to assist research on complex biological systems.","PeriodicalId":33241,"journal":{"name":"Advanced Photonics","volume":"142 1","pages":"0"},"PeriodicalIF":20.6000,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Advanced Photonics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1117/1.ap.5.6.066001","RegionNum":1,"RegionCategory":"物理与天体物理","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"OPTICS","Score":null,"Total":0}
引用次数: 0
Abstract
Stimulated Raman scattering (SRS) microscopy has shown superior chemical resolution due to the much narrower vibrational spectral bandwidth than its fluorescence counterpart. However, breaking the diffraction-limited spatial resolution of SRS imaging is much more challenging because of the intrinsically weak scattering cross section and inert/stable nature of molecular bond vibrations. We report superresolution SRS (SR-SRS) nanoscopy based on reversible-switchable vibrational photochromic probes integrated with point spread function engineering strategy. By introducing a Gaussian-shaped ultraviolet excitation beam and a donut-shaped visible depletion beam in addition to the pump and Stokes beams, SR-SRS could reach sub-100 nm resolution on photoswitchable nanoparticles (NPs). Furthermore, NP-treated live cell imaging was demonstrated with resolution improvement by a factor of ∼4. Our proof-of-principle work provides the potential for SR vibrational imaging to assist research on complex biological systems.
期刊介绍：
Advanced Photonics is a highly selective, open-access, international journal that publishes innovative research in all areas of optics and photonics, including fundamental and applied research. The journal publishes top-quality original papers, letters, and review articles, reflecting significant advances and breakthroughs in theoretical and experimental research and novel applications with considerable potential.
The journal seeks high-quality, high-impact articles across the entire spectrum of optics, photonics, and related fields with specific emphasis on the following acceptance criteria:
-New concepts in terms of fundamental research with great impact and significance
-State-of-the-art technologies in terms of novel methods for important applications
-Reviews of recent major advances and discoveries and state-of-the-art benchmarking.
The journal also publishes news and commentaries highlighting scientific and technological discoveries, breakthroughs, and achievements in optics, photonics, and related fields.
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