Phytochemical, Physicochemical and HPTLC Analysis of Siddha Herbal Formulation Muppirandai Chooranam

Abstract

Aim: The aim of the study was to evaluate the Phytochemical, Physicochemical, HPTLC analysis of Siddha herbal formulation Muppirandai Chooranam (MRC), indicated for various ailments including menorrhagia. Study design Place of study: The Phytochemical and Physicochemical analysis were conducted at the Tamilnadu Dr. MGR Medical University, located at no.69, Anna salai, Guindy, Chennai-32. The high-performance thin layer chromatography (HPTLC) was carried out at Noble research solutions, Kolathur, Chennai -99. Methodology: Siddha formulation Muppirandai Chooranam was prepared as per good manufacturing practices (GMP) guidelines and the phytochemical analysis, physicochemical analysis were carried out at the Tamilnadu Dr. MGR Medical University, located at no.69, Anna salai, Guindy, Chennai-32. As per PLIM guidelines (Pharmacopeia Laboratory Of Indian Medicine) in accordance with the guidelines established by AYUSH (Ayurveda, Yoga, Unani, Siddha, Homoeopathy), the governing body for traditional health systems in India. The HPTLC analysis was conducted at Noble Research Solutions, Kolathur, Chennai -99. Results: The Phytochemical screening of MRC shown the presence of Alkaloids, Carbohydrates, Saponins, Flavanoids, Diterpenes, Gum and Mucilage. The physicochemical analysis of MRC revealed that it had a loss on drying of 0.42% at 105°C, a total ash content of 23.3%, an acid-insoluble ash content of 3.02%, a water-soluble ash content of 2.30%, and a water-soluble extract content of 17.67%, and alcohol soluble extract content of 5.23%.HPTLC finger printing analysis of the sample reveals the presence of five prominent peaks corresponds to the presence of five versatile phytocomponents present within it. Rf value of the peaks ranges from 0.02 to 0.37. Conclusion: From the findings, it is concluded that it shown the compendious understanding of presence of Phytochemical components, Physicochemical characteristics, and HPTLC analysis of MRC and it is competent to assess the quality profile of Muppirandai Chooranam as a reference standard for the development of the standardised pharmaceutical product.