[Detection of oligoclonal immunoglobulins in the cerebrospinal fluid using agar gel immunoelectrophoresis with silver salt staining].

H Merahba, C Allal, M Ghaffor, H Rabhi, M C Abbadi
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Abstract

Detection of oligoclonal immunoglobulins in multiple sclerosis cerebrospinal fluid (MS-CSF) seems to be an important test in the biological diagnosis of the disease. In our laboratory, the classical agarose gel electrophoretic technic allowed the detection of CSF oligoclonal bands in only 40% of the MS patients. This relatively low percentage in comparison with those obtained by other investigators led us to develop a much more resolving electrophoretic technic: an agarose gel isoelectric focusing (IEF) with silver staining. By this method, we detected oligoclonal immunoglobulins in 43 (43%) of 100 MS-CSF exhibiting only polyclonal patterns on classical electrophoresis. Oligoclonal banding appeared particularly in patients with inflammatory type profile (36/60) according to Schuller's classification, but also in those with normal (5/26) or inflammatory transudative (2/8) type profiles. MS-CSF with oligoclonal immunoglobulins on IEF had a higher IgG/Albumin ratio (p = 0.01) than those with polyclonal immunoglobulins, while the mean IgG levels were not significantly different (p = 0.07). These results support the diagnostic usefulness of the IgG/Albumin ratio. Agarose IEF appears to be a useful technic in the detection of oligoclonal immunoglobulins and may be applied at least to CSF of patients with clinical signs of multiple sclerosis.

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【银盐染色琼脂凝胶免疫电泳检测脑脊液中寡克隆免疫球蛋白】。
多发性硬化症脑脊液(MS-CSF)中寡克隆免疫球蛋白的检测似乎是该疾病生物学诊断的重要检测方法。在我们的实验室中,经典的琼脂糖凝胶电泳技术仅允许在40%的MS患者中检测CSF寡克隆带。与其他研究者相比,这一相对较低的百分比使我们开发了一种更具分辨性的电泳技术:琼脂糖凝胶等电聚焦(IEF)与银染色。通过这种方法,我们在100个MS-CSF中检测到43个(43%)的低克隆免疫球蛋白,在经典电泳上仅显示多克隆模式。寡克隆带状带主要出现在Schuller分类的炎症型(36/60)患者中,但也出现在正常(5/26)或炎症变性(2/8)型患者中。低克隆免疫球蛋白MS-CSF在IEF上的IgG/Albumin比值高于多克隆免疫球蛋白MS-CSF (p = 0.01),而IgG的平均水平差异无统计学意义(p = 0.07)。这些结果支持IgG/白蛋白比值的诊断价值。琼脂糖IEF似乎是一种检测寡克隆免疫球蛋白的有用技术,至少可以应用于多发性硬化症临床症状患者的脑脊液。
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