Supercritical extract from the Japanese sea brown algae <i>Undaria pinnatifida</i> as a source of bioactive compounds

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY Izvestiya Vuzov-Prikladnaya Khimiya i Biotekhnologiya Pub Date : 2023-09-30 DOI:10.21285/2227-2925-2023-13-3-416-424
O. V. Tabakaeva, A. V. Tabakaev
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Abstract

Bioactive compounds contained in algae have practical applications in the pharmaceutical industry, production of human food and animal feed, and other fields. Since brown algae contain a wide range of bioactive substances, producing high-quality and chemically safe extracts on their basis is an urgent task. This study was aimed at characterization of supercritical extract from the corresponding algae, as well as the identification and evaluation of the content of bioactive compounds. The content of carotenoids, phenolic compounds and mannitol was determined by UV-Vis spectroscopy; a fatty acid analysis was carried out by GLPC; the qualitative composition of carotenoids and phenolic compounds was analyzed by HPLC. Supercritical CO 2 extraction of the brown alga U. rinnatifida with EtOH as a polar modifier is characterized by high ejection efficiency of fatty acids, phenolic compounds and carotenoids. The latter profile of the collected supercritical extract consists of 14 representatives. In particular, fucoxanthin (58.1% of the sum), zeaxanthin (12.6% of the sum), and fucoxanthinol (14.5% of the sum) are the most abundant compounds. Palmitic acid, oleic acid, arachidonic acid, and eicosapentaenoic acids are the main of the 20 fatty acids found in the corresponding extract. However, the class of polyunsaturated fatty acids is predominant, which content of the ώ-6 and ώ-3 groups does not differ significantly. The total content of phenolic compounds is 13.45+0.43 mg/g of gallic acid equivalent. For instance, the most represented phenolic substances are epicatechin, epigallocatechin gallate, syringic acid, coumaric acid, ferulic acid and salicylic acid.
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日本海褐藻<i>裙带藻</i>作为生物活性化合物的来源
藻类中含有的生物活性化合物在制药工业、人类食品和动物饲料生产等领域都有实际应用。由于褐藻含有广泛的生物活性物质,在其基础上生产高质量和化学安全的提取物是一项紧迫的任务。本研究旨在对相应藻类的超临界提取物进行表征,并对其生物活性化合物的含量进行鉴定和评价。紫外-可见光谱法测定了类胡萝卜素、酚类化合物和甘露醇的含量;采用GLPC法进行脂肪酸分析;用高效液相色谱法分析了类胡萝卜素和酚类化合物的定性组成。以乙醇为极性改性剂对褐藻进行超临界co2萃取,其脂肪酸、酚类化合物和类胡萝卜素的萃取效率高。所收集的超临界萃取物的后一个剖面由14个代表组成。其中,岩藻黄质(58.1%)、玉米黄质(12.6%)和岩藻黄醇(14.5%)是含量最多的化合物。棕榈酸、油酸、花生四烯酸和二十碳五烯酸是在相应提取物中发现的20种脂肪酸中的主要成分。然而,多不饱和脂肪酸类占主导地位,其含量ώ-6和ώ-3组没有显著差异。总酚类化合物含量为13.45+0.43 mg/g没食子酸当量。例如,最具代表性的酚类物质是表儿茶素、表没食子儿茶素没食子酸酯、丁香酸、香豆酸、阿魏酸和水杨酸。
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来源期刊
自引率
50.00%
发文量
63
审稿时长
12 weeks
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