Carotenoid content in <i>Prunus armeniaca</i> L. apricot fruits depending on the extraction method

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY Izvestiya Vuzov-Prikladnaya Khimiya i Biotekhnologiya Pub Date : 2023-09-30 DOI:10.21285/2227-2925-2023-13-3-402-408
M. V. Semenova, A. G. Kuklina, V. V. Kondratieva, L. S. Olekhnovich, T. V. Voronkova
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Abstract

In the middle of the 20th century, specialists of the Tsitsin Main Moscow Botanical Garden of the Russian Academy of Sciences collected a valuable apricot gene pool characterized by high diversity and winter hardiness. From this gene pool, L.A. Kramarenko carried out selection work to develop winter-hardy apricot varieties, such as Aisberg, Vodoley, Guiani, Lel’, Tsarsky and others, which were included in the State Register of Breeding Achievements of the Russian Federation. The use of these varieties in further selective breeding requires information on their carotenoid content. In this work, we determine carotenoids in apricot (Prunus armeniaca L., Rosaceae) fruits of Moscow breeding varieties Aisberg, Vodoley, Guiani, Lel’, Tsarsky and Zachat’ievsky. We employed different extraction methods of plant raw materials, contributing to the optimal extraction of carotenoids from apricot fruits. Extraction was performed with hexane, 96% ethanol at room temperature, and EtOH heated to 60°C (water bath) for 40 min. The highest carotenoid content was observed in Lel’ (31.73 mg%) and Vodoley (31.77 mg%) varieties, while the lowest content was observed in the Zachat’ievsky sample (11.93 mg%). The application of multiple solvents increases the reliability of determining carotenoids in plants for computational and experimental studies. The discovered range of their content can be used for description of promising forms during breeding works. In this way, new and beneficial for health varieties may emerge.
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<i>杨梅</i>L.杏果实依提取方法而定
20世纪中叶,俄罗斯科学院莫斯科茨辛植物园的专家收集了一个具有高度多样性和耐寒性的珍贵杏基因库。从这个基因库中,la Kramarenko开展了选育工作,培育出了Aisberg、Vodoley、Guiani、Lel’、Tsarsky等耐寒杏品种,这些品种被列入俄罗斯联邦国家育种成果登记册。在进一步选择育种中使用这些品种需要有关其类胡萝卜素含量的信息。在这项工作中,我们测定了莫斯科育种品种Aisberg, Vodoley, Guiani, Lel ', Tsarsky和Zachat ' ievsky杏(Prunus armeniaca L., Rosaceae)果实中的类胡萝卜素。采用不同的植物原料提取方法,优选出杏果实中类胡萝卜素的最佳提取方法。以己烷、96%乙醇在室温下提取,乙醇加热60℃(水浴)提取40 min。Lel’和Vodoley品种类胡萝卜素含量最高(31.73 mg%),而Zachat’ievsky样品含量最低(11.93 mg%)。多种溶剂的应用增加了计算和实验研究中测定植物中类胡萝卜素的可靠性。其发现的内容范围可用于育种工作中对有前途的形态的描述。这样,对健康有益的新品种就会出现。
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来源期刊
自引率
50.00%
发文量
63
审稿时长
12 weeks
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