Quantitative Analysis of Cannabidiol and Δ9-Tetrahydrocannabinol Contents in Different Tissues of Four Cannabis Cultivars using Gas Chromatography-Mass Spectrometry

IF 1 4区农林科学 Q3 HORTICULTURE Korean Journal of Horticultural Science & Technology Pub Date : 2023-06-30 DOI:10.7235/hort.20230031

Young Mo Koo, S. M. Ahsan, Da Bin Kwon, Hyuk Jung, Jeong Doo Choi, Myung Soo Lee, Kiu-Hyung Cho, Byung-Chun In, Hyong Woo Choi

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引用次数: 2

Abstract

In this study, we analyzed cannabidiol (CBD) and Δ9-tetrahydrocannabinol (THC) contents in different tissues of four cannabis cultivars, Cheongsam, Cherry Blossom, Hot Blonde, and Queen Dream, using gas chromatography-mass spectrometry (GC-MS). Standard calibration curves for CBD and THC were generated by analyzing standard CBD and THC solutions via GC-MS, respectively. GC-MS analyses of different tissues of the Cheongsam cultivar revealed different levels of CBD and THC depending on the tissues tested. Female flowers accumulated the highest CBD and THC levels at 0.052±0.02 and 0.02±0.005%, respectively, in the Cheongsam cultivar. Similarly, female flowers of the Cherry Blossom, Hot Blonde, and Queen Dream cultivars accumulated the highest amounts of CBD (2.45% to 3.54%) and THC (0.15% to 0.18%) compared to other tissues, such as the leaves and stems. These results suggest that female flowers of cannabis plants show higher potency levels for CBD and THC production compared to other tissues.

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四种大麻品种不同组织中大麻二酚和Δ9-Tetrahydrocannabinol含量的气相色谱-质谱定量分析

本研究采用气相色谱-质谱联用技术分析了旗袍、樱花、金发辣妹和皇后梦4个大麻品种不同组织中大麻二酚(CBD)和Δ9-tetrahydrocannabinol (THC)的含量。通过GC-MS分别对CBD和THC标准溶液进行分析，生成CBD和THC的标准校准曲线。对旗袍品种不同组织的GC-MS分析显示，不同组织的CBD和THC含量不同。旗袍品种雌花CBD和THC含量最高，分别为0.052±0.02和0.02±0.005%。同样，与其他组织(如叶和茎)相比，樱花、性感金发和皇后梦品种的雌花积累的CBD(2.45%至3.54%)和四氢大麻酚(0.15%至0.18%)含量最高。这些结果表明，与其他组织相比，大麻植物的雌花显示出更高的CBD和四氢大麻酚生产能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Korean Journal of Horticultural Science & Technology 农林科学-园艺

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provides scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.