Anisha Valsalam, K. V. Rajendran, Pooja Vinde, Megha Kadam Bedekar
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引用次数: 0
Abstract
DNA vaccines are the most essential tool of the disease prevention strategy. In the present study, cloning of a heterologous fusion gene involving glyceraldehyde 3-phosphate dehydrogenase and interferon gamma (GAPDH-IFN) was conceptualised. Cloning was tried using four distinct transformation techniques viz. InstAclone PCR Cloning kit (Fermentas, USA); CaCl2 transformation protocol; Clontech stellar competent cells protocol and PEG 8000-mediated transformation method, for the heterologous GAPDH-IFN fusion gene using DH5α strain of Escherichia coli. The first three methods were found to be unsuitable, and the PEG 8000-mediated transformation method yielded positive clones. Keywords: DNA vcaccine, Edwardsiella tarda, Fusion genes, Labeo rohita, PEG 8000, Transformation methods
期刊介绍：
Indian Journal of Fisheries is published quarterly by the Indian Council of Agricultural Research (ICAR), New Delhi. Original contributions in the field of Fish and fisheries science are considered for publication in the Journal. The material submitted must be unpublished and not under consideration for publication elsewhere.
Papers based on research which kills or damages any species, regarded as thratened/ endangered by IUCN crieteria or is as such listed in the Red Data Book appropriate to the geographic area concerned, will not be accepted by the Journal, unless the work has clear conservation objectives.
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