This study reports the experimental results carried out to optimise containers and miltdilution ratios for carp sperm cryopreservation, in order to make the process simple andcompatible with the needs of commercial carp seed production, which is a small-scale andlow technology sector in Asia. The procedure optimisation for up-scaling was assessedby the ability of cryopreserved sperms to fertilise Cyprinsu carpio eggs and hatchingpercentage, in three different experiments. In the first experiment, we tested the effect ofmilt to different extender dilutions (1:4, 1:6 and 1:8); the second experiment tested theeffect of container capacities (0.5 ml French straw; 2 ml and 5 ml cryovials) with two dilutionratios (1:3 and 1:6) and in the third experiment, the efficacy of pre-mix of extender salts andindividually pre-weighed salts stored over 5 months was compared with fresh preparationof extender. Out of the three dilutions tested, two dilutions viz., 1:4 and 1:6, yielded hatchingabove 50%. We also used the milt cryopreserved at dilution of 1:3 which was post-thawdiluted with extender, to make final dilution of 1:6, to fertilise the eggs. This post-thawdilution in the ratio 1:6, yielded the results comparable to the milt cryopreserved in 1:6dilution. This approach can help small-scale seed producers to store more sperm withinthe limits of the cost of liquid nitrogen. The extender salt composition, pre-weighed andpre-mixed, was also found to give comparable results to the fresh composition after storageover 5 months. This can assist in developing ready to use, cost-effective, working kitsavailable to semi-technical hatchery seed producers. The parameters optimised in the studyhas potential to be transformed as an easy strategy with the use of 5 ml vial, cryopreservedat 1:3 dilution, with further post-thaw dilution at site, thus aiding in fertilisation of largevolume of eggs produced by high fecund carp species within the limited time available formaintaining good gamete quality. Keywords:Cryobanking, Cryovials, Dilution, Salt premix
{"title":"Optimisation of cryovials and milt dilution ratios to upscale carp sperm cryopreservation for use in hatchery seed production","authors":"Santosh Kumar, Aditya Kumar, Rama Shankar Sah, Ajay Kumar Singh, Ajay Yadav, Arvind Verma, Deepti Negi, Hayin Tamut, Kuldeep Kumar Lal","doi":"10.21077/ijf.2023.70.3.134401-12","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.134401-12","url":null,"abstract":"This study reports the experimental results carried out to optimise containers and miltdilution ratios for carp sperm cryopreservation, in order to make the process simple andcompatible with the needs of commercial carp seed production, which is a small-scale andlow technology sector in Asia. The procedure optimisation for up-scaling was assessedby the ability of cryopreserved sperms to fertilise Cyprinsu carpio eggs and hatchingpercentage, in three different experiments. In the first experiment, we tested the effect ofmilt to different extender dilutions (1:4, 1:6 and 1:8); the second experiment tested theeffect of container capacities (0.5 ml French straw; 2 ml and 5 ml cryovials) with two dilutionratios (1:3 and 1:6) and in the third experiment, the efficacy of pre-mix of extender salts andindividually pre-weighed salts stored over 5 months was compared with fresh preparationof extender. Out of the three dilutions tested, two dilutions viz., 1:4 and 1:6, yielded hatchingabove 50%. We also used the milt cryopreserved at dilution of 1:3 which was post-thawdiluted with extender, to make final dilution of 1:6, to fertilise the eggs. This post-thawdilution in the ratio 1:6, yielded the results comparable to the milt cryopreserved in 1:6dilution. This approach can help small-scale seed producers to store more sperm withinthe limits of the cost of liquid nitrogen. The extender salt composition, pre-weighed andpre-mixed, was also found to give comparable results to the fresh composition after storageover 5 months. This can assist in developing ready to use, cost-effective, working kitsavailable to semi-technical hatchery seed producers. The parameters optimised in the studyhas potential to be transformed as an easy strategy with the use of 5 ml vial, cryopreservedat 1:3 dilution, with further post-thaw dilution at site, thus aiding in fertilisation of largevolume of eggs produced by high fecund carp species within the limited time available formaintaining good gamete quality. Keywords:Cryobanking, Cryovials, Dilution, Salt premix","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139331926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.131143-18
Sanjay Kumar, Sevok Das, Chandan Handique, Debnath, S. G. Singh, Prasanta Mahanta
Critical thermal maxima (CTmax), lethal temperature maxima (LTmax), opercular respirationrate (ORR - movement of operculum per minute), temperature quotient (Q10) and stressmarkers of Osteobrama belangeri (Pengba), a high-value medium carp endemic tohilly ecosystems of North-east India, were examined with the aim of understanding itsaquaculture potential and management requirements in the event of a temperature rise.Fish (7.82±0.35 cm/4.64±0.36 g) were acclimatised at 20, 25 and 30°C in insulated tanks(10 fish per tank) for 30 days. They were then exposed to a water temperature increase(@ 1°C day-1) using a locally-fabricated thermostat and assessed for CTmax, LTmax and ORR.As the acclimation temperature increased from 20 to 30°C, CTmax, LTmax and ORR of thefish significantly increased. Regression analysis indicated a strong positive correlationbetween acclimation temperatures and CTmax (y = 0.22x + 33.55, R² = 0.97) and LTmax(y = 0.27x + 32.86, R² = 0.99). Fish blood samples were collected at the endpoints ofCTmax and LTmax and analysed for serum alkaline phosphatase (ALP) activity. The resultsindicated that the fish experienced maximum stress at an acclimation temperature of 30°C(ALP: 38.7 U l-1), followed by 25°C (35.6 U l-1) and 20°C (31.7 U l-1). The results suggestedthat a water temperature increase above 30°C can impair the physiology of O. belangeri andconsequently, their reproductive competence. Therefore, in such events, proper managementand additional care are necessary to protect them from extinction. Keywords:Critical thermal maxima, Lethal temperaturemaxima, Osteobrama belangeri, Thermal limit,Temperature quotient
{"title":"Assessment of Osteobrama belangeri (Pengba), a high-value medium carp endemic to North-east India for thermal tolerance limits","authors":"Sanjay Kumar, Sevok Das, Chandan Handique, Debnath, S. G. Singh, Prasanta Mahanta","doi":"10.21077/ijf.2023.70.3.131143-18","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.131143-18","url":null,"abstract":"Critical thermal maxima (CTmax), lethal temperature maxima (LTmax), opercular respirationrate (ORR - movement of operculum per minute), temperature quotient (Q10) and stressmarkers of Osteobrama belangeri (Pengba), a high-value medium carp endemic tohilly ecosystems of North-east India, were examined with the aim of understanding itsaquaculture potential and management requirements in the event of a temperature rise.Fish (7.82±0.35 cm/4.64±0.36 g) were acclimatised at 20, 25 and 30°C in insulated tanks(10 fish per tank) for 30 days. They were then exposed to a water temperature increase(@ 1°C day-1) using a locally-fabricated thermostat and assessed for CTmax, LTmax and ORR.As the acclimation temperature increased from 20 to 30°C, CTmax, LTmax and ORR of thefish significantly increased. Regression analysis indicated a strong positive correlationbetween acclimation temperatures and CTmax (y = 0.22x + 33.55, R² = 0.97) and LTmax(y = 0.27x + 32.86, R² = 0.99). Fish blood samples were collected at the endpoints ofCTmax and LTmax and analysed for serum alkaline phosphatase (ALP) activity. The resultsindicated that the fish experienced maximum stress at an acclimation temperature of 30°C(ALP: 38.7 U l-1), followed by 25°C (35.6 U l-1) and 20°C (31.7 U l-1). The results suggestedthat a water temperature increase above 30°C can impair the physiology of O. belangeri andconsequently, their reproductive competence. Therefore, in such events, proper managementand additional care are necessary to protect them from extinction. Keywords:Critical thermal maxima, Lethal temperaturemaxima, Osteobrama belangeri, Thermal limit,Temperature quotient","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.134706-16
S. Tanuja, A. Sarkar, G. Moharana, C. S. Mhatre
Homestead integrated aquaculture has immense potential towards alleviating poverty andundernourishment. In order to have sustainable and equitable impacts through small holderaquaculture innovations, a focus on gender being included at the very start of the designand dissemination is required. The present case study showcases the impact of genderinclusive interventions in improving the participation of women in homestead aquaculture,thereby improving the household fish and vegetable availability and income. Conservation ofthe diminishing stock of small indigenous fishes by deliberately farming them along with theIndian major carps (IMCs) was also a focus of the study. A gender inclusive approach wasadopted in the capacity building of women on scientific aquaculture management practices.Twenty four homestead ponds having areas ranging from 0.17-0.22 ha in Puri District, Odishacovering a total area of 4 ha from four villages were selected to undertake participatoryaction research. The ponds were stocked with IMCs @10000 ha-1 and small indigenousfishes like Amblyphanrynogon mola and Puntius spp. @25000 ha-1. Several women friendlyinterventions like gillnets (mesh size 12 mm) a passive fishing gear and trellis systemaround pond bund for growing climber vegetables were introduced in the villages with anaim to improve the participation of women in homestead integrated aquaculture. As a resultof the interventions, the fish production increased from 0.75 t ha-1 yr-1 to 2.48 t ha-1 yr-1.The involvement of women in harvesting of small fish using passive gear like gillnets andtraps resulted in regular fortnightly availability of around 750 g of small micronutrient richfish to rural families. The utilisation of pond bund for growing vegetables following thecropping calendar resulted in production around 790 kg vegetables per hectare of pondarea. The study showed that women can immensely contribute to increasing and stabilisingfamily income and in improving family nutrition by participating in homestead integratedaquaculture. The research also proved that polyculture of IMCs with small indigenous fishesby following the scientific management practices will result in better yield, improved fishavailability to the house and better management of the household resources. Keywords: Aquaculture, Empowerment, Household, Horticulture, Participation, Women
{"title":"Improving household fish and vegetable availability through participation of rural women: A case study","authors":"S. Tanuja, A. Sarkar, G. Moharana, C. S. Mhatre","doi":"10.21077/ijf.2023.70.3.134706-16","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.134706-16","url":null,"abstract":"Homestead integrated aquaculture has immense potential towards alleviating poverty andundernourishment. In order to have sustainable and equitable impacts through small holderaquaculture innovations, a focus on gender being included at the very start of the designand dissemination is required. The present case study showcases the impact of genderinclusive interventions in improving the participation of women in homestead aquaculture,thereby improving the household fish and vegetable availability and income. Conservation ofthe diminishing stock of small indigenous fishes by deliberately farming them along with theIndian major carps (IMCs) was also a focus of the study. A gender inclusive approach wasadopted in the capacity building of women on scientific aquaculture management practices.Twenty four homestead ponds having areas ranging from 0.17-0.22 ha in Puri District, Odishacovering a total area of 4 ha from four villages were selected to undertake participatoryaction research. The ponds were stocked with IMCs @10000 ha-1 and small indigenousfishes like Amblyphanrynogon mola and Puntius spp. @25000 ha-1. Several women friendlyinterventions like gillnets (mesh size 12 mm) a passive fishing gear and trellis systemaround pond bund for growing climber vegetables were introduced in the villages with anaim to improve the participation of women in homestead integrated aquaculture. As a resultof the interventions, the fish production increased from 0.75 t ha-1 yr-1 to 2.48 t ha-1 yr-1.The involvement of women in harvesting of small fish using passive gear like gillnets andtraps resulted in regular fortnightly availability of around 750 g of small micronutrient richfish to rural families. The utilisation of pond bund for growing vegetables following thecropping calendar resulted in production around 790 kg vegetables per hectare of pondarea. The study showed that women can immensely contribute to increasing and stabilisingfamily income and in improving family nutrition by participating in homestead integratedaquaculture. The research also proved that polyculture of IMCs with small indigenous fishesby following the scientific management practices will result in better yield, improved fishavailability to the house and better management of the household resources. Keywords: Aquaculture, Empowerment, Household, Horticulture, Participation, Women","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.117778-06
P. L. Mahadwala, V. Nirmale, S. Metar, B. P. Bhosale, R. A. Pawar, N. D. Chogale
In the present study, we investigated some biological aspects of the spineless cuttlefish,Sepiella inermis (Orbigny, 1848) along the Ratnagiri coast of Maharashtra, India. A study ondiet composition and reproductive biology of the species was carried out. Dorsal mantlelength (DML) ranged from 35.9 to 93.3 mm for males and 29.3 to 98 mm for females.Feeding intensity was noted to be low during most of the study period. S. inermis was foundto have a protracted spawning season. The overall male: female ratio was 1:1.02 during thestudy period. The average fecundity was found to be 358 eggs. Length at sexual maturitywas estimated to be 42 mm and 53 mm for males and females respectively. The informationobtained from the study will help the fishery agencies to formulate management measuresin sustaining the landings of S. inermis in the region. Keywords:Biological studies, Cephalopods, Maharashtra,Ratnagiri coast, Sepiella inermis, Spinelesscuttlefish
{"title":"Diet composition and reproductive biology of spineless cuttlefish Sepiella inermis (Orbigny, 1848) from Ratnagiri (Arabian Sea, North-west coast of India)","authors":"P. L. Mahadwala, V. Nirmale, S. Metar, B. P. Bhosale, R. A. Pawar, N. D. Chogale","doi":"10.21077/ijf.2023.70.3.117778-06","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.117778-06","url":null,"abstract":"In the present study, we investigated some biological aspects of the spineless cuttlefish,Sepiella inermis (Orbigny, 1848) along the Ratnagiri coast of Maharashtra, India. A study ondiet composition and reproductive biology of the species was carried out. Dorsal mantlelength (DML) ranged from 35.9 to 93.3 mm for males and 29.3 to 98 mm for females.Feeding intensity was noted to be low during most of the study period. S. inermis was foundto have a protracted spawning season. The overall male: female ratio was 1:1.02 during thestudy period. The average fecundity was found to be 358 eggs. Length at sexual maturitywas estimated to be 42 mm and 53 mm for males and females respectively. The informationobtained from the study will help the fishery agencies to formulate management measuresin sustaining the landings of S. inermis in the region. Keywords:Biological studies, Cephalopods, Maharashtra,Ratnagiri coast, Sepiella inermis, Spinelesscuttlefish","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139333288","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An experiment for 90 days was performed to optimise the stocking density of pangasius(Pangasianodon hypophthalmus) with basil (Ocimum basilicum L.) in a nutrient film technique(NFT) based aquaponics. The experiment had four treatments with different stockingdensities of pangasius as T1 (2.5 kg m-3), T2 (2.75 kg m-3), T3 (3.0 kg m-3) and T4 (3.25 kg m-3)with a constant plant density of 24 plants m-2 and control viz., C (2.5 kg m-3 withoutplants). Significant (p<0.05) difference in final body weight of fish was found among thetreatments and control. The highest fish biomass was recorded in T3 (10.29±4.41 kg m-3)followed by T4, T2, T1 and C. The highest plant yield was found in T4 (510.90±6.25 g);however, no significant difference was found between T4 and T3. No significant (p>0.05)difference in macro and micronutrient content of basil leaves was recorded in T4 and T3.The physiological parameters (hematological, serum biochemical and anti-oxidant stressenzymes) were found to be within the acceptable range. Considering the water qualityparameters, fish growth, total biomass of fish, fish physiological responses, basil yield andnutrient content, the stocking density of 3.00 kg m-3 (pangasius) with 24 plants m-2 of basilcould be recommended for basil-pangasius aquaponics. Keywords:Aquaponics, Aquaculture wastewater, Basil,Nutrient film technique, Pangasius
{"title":"Stocking density optimisation of pangasius (Pangasianodon hypophthalmus) fingerlings with basil (Ocimum basilicum L.) in nutrient film technique (NFT) based aquaponics","authors":"Neerudu Harika, Ajit Kumar Verma, Kishore Kumar, Chandrakant Mallikarjun Krishnani, Tincy Hittinahalli, V. Varghese, Shree Bharti Angom Lenin, Singh Aatira, Farooq","doi":"10.21077/ijf.2023.70.3.136073-09","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.136073-09","url":null,"abstract":"An experiment for 90 days was performed to optimise the stocking density of pangasius(Pangasianodon hypophthalmus) with basil (Ocimum basilicum L.) in a nutrient film technique(NFT) based aquaponics. The experiment had four treatments with different stockingdensities of pangasius as T1 (2.5 kg m-3), T2 (2.75 kg m-3), T3 (3.0 kg m-3) and T4 (3.25 kg m-3)with a constant plant density of 24 plants m-2 and control viz., C (2.5 kg m-3 withoutplants). Significant (p<0.05) difference in final body weight of fish was found among thetreatments and control. The highest fish biomass was recorded in T3 (10.29±4.41 kg m-3)followed by T4, T2, T1 and C. The highest plant yield was found in T4 (510.90±6.25 g);however, no significant difference was found between T4 and T3. No significant (p>0.05)difference in macro and micronutrient content of basil leaves was recorded in T4 and T3.The physiological parameters (hematological, serum biochemical and anti-oxidant stressenzymes) were found to be within the acceptable range. Considering the water qualityparameters, fish growth, total biomass of fish, fish physiological responses, basil yield andnutrient content, the stocking density of 3.00 kg m-3 (pangasius) with 24 plants m-2 of basilcould be recommended for basil-pangasius aquaponics. Keywords:Aquaponics, Aquaculture wastewater, Basil,Nutrient film technique, Pangasius","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.129755-19
J. Mohanty, Sonalina Sahoo, M. Badhe, I. Das, Priyanka Das, A. Rasal, K. Murmu, K. Mahapatra, Pramoda Kumar Sahoo, J. Sundaray
Incubation of fertilised eggs from the Indian major carp species, Catla catla was carriedout in tissue culture microplates to assess the quality in terms of hatching rate and posthatchsurvival rate. Eggs from five females (F1, F2, F3, F5 and F6) were strip spawned andfertilised with milt from a single male. The fertilised eggs were distributed at one egg perwell of 24 well microplates, with three plates for each female. The plates were incubated at30oC in an incubator with continuous shaking and complete hatching was observed by 36 h.The hatching rate varied within a range of 0 to 52.8% showing the maximum of 5% for F5followed by F1 (50%) which were significantly higher than the other three females (F2-18.1,F6-5.6 and F3-0%). The eggs from these two females (F1 and F5) thus could be consideredas good. The survival percentage of hatchlings recorded for a period of 8 days post-hatchingwas found to vary from 58.3 to 83.3 and there was low variability among different females onany observed day. Between these two egg quality determinants, the hatching rate was foundto be more suitable considering the wide variability shown by different females indicatingthe quality difference. The microplate method seems to be an appropriate tool to assess theegg quality in catla and can be extended to other species as well. Keywords:Catla catla, Egg quality, Hatching rate, Microplate
{"title":"Microplate-based incubation method for quality assessment of eggs of Indian major carp Catla catla","authors":"J. Mohanty, Sonalina Sahoo, M. Badhe, I. Das, Priyanka Das, A. Rasal, K. Murmu, K. Mahapatra, Pramoda Kumar Sahoo, J. Sundaray","doi":"10.21077/ijf.2023.70.3.129755-19","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.129755-19","url":null,"abstract":"Incubation of fertilised eggs from the Indian major carp species, Catla catla was carriedout in tissue culture microplates to assess the quality in terms of hatching rate and posthatchsurvival rate. Eggs from five females (F1, F2, F3, F5 and F6) were strip spawned andfertilised with milt from a single male. The fertilised eggs were distributed at one egg perwell of 24 well microplates, with three plates for each female. The plates were incubated at30oC in an incubator with continuous shaking and complete hatching was observed by 36 h.The hatching rate varied within a range of 0 to 52.8% showing the maximum of 5% for F5followed by F1 (50%) which were significantly higher than the other three females (F2-18.1,F6-5.6 and F3-0%). The eggs from these two females (F1 and F5) thus could be consideredas good. The survival percentage of hatchlings recorded for a period of 8 days post-hatchingwas found to vary from 58.3 to 83.3 and there was low variability among different females onany observed day. Between these two egg quality determinants, the hatching rate was foundto be more suitable considering the wide variability shown by different females indicatingthe quality difference. The microplate method seems to be an appropriate tool to assess theegg quality in catla and can be extended to other species as well. Keywords:Catla catla, Egg quality, Hatching rate, Microplate","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139331683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.131139-01
S. Surya, R. Prathibha, E. M. Abdusammad, T. L. Asha, B. Santhosh, B. B. Nayak, N. S. Jeena, K. G. Mini, Shubhadeep Ghosh, J. H. Kingsly, P. A. Azeez, C. Anulekshmi, R. Karankumar, J. M. Ponni, G. Angel, M. K. Anil
Sailfishes, marlins, spearfishes and swordfishes commonly referred to as billfishes are highlymigratory species, with a worldwide distribution in tropical and subtropical oceans. Thelandings of billfishes along the Indian coast registered an increasing trend with an estimatedlanding of 14,759 t in 2019. Kerala (41%) contributed the maximum followed by Tamil Nadu(28%), Andhra Pradesh (18%), Gujarat (8%) and Maharashtra (2%) to the total billfish landingsduring the last decade. Mechanised gillnetter cum hook and line was the major gear landingbillfishes. The major species landed during 2012-2019 were Istiophorus platypterus (52.2%),Istiompax indica (21.1%), Makaira nigricans (7.3%), Xiphias gladius (17.2%) and Kajikia audax(2.3%). Four of these species could be easily distinguished by COI barcodes but, the stripedmarlin, K. audax showed high sequence similarity with K. albida and cannot be distinguishedby barcodes alone. Control region (889 bp) provided a better phylogenetic signal, consistentwith that of the whole mitochondrial genome topology. The stock status plots of billfishesdepicted that, all the species were in the developing and exploited phase. Growth, mortalityand exploitation rates estimated for four billfishes indicated that the present fishing ratesand biomass levels are at safe levels and there is considerable scope for enhancing theirfishery. Keywords:CO1 barcodes, Exploitation rate, Large pelagics,Life history, Stock status plots
{"title":"Fishery, systematics and stock dynamics of billfishes landed along the Indian coast","authors":"S. Surya, R. Prathibha, E. M. Abdusammad, T. L. Asha, B. Santhosh, B. B. Nayak, N. S. Jeena, K. G. Mini, Shubhadeep Ghosh, J. H. Kingsly, P. A. Azeez, C. Anulekshmi, R. Karankumar, J. M. Ponni, G. Angel, M. K. Anil","doi":"10.21077/ijf.2023.70.3.131139-01","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.131139-01","url":null,"abstract":"Sailfishes, marlins, spearfishes and swordfishes commonly referred to as billfishes are highlymigratory species, with a worldwide distribution in tropical and subtropical oceans. Thelandings of billfishes along the Indian coast registered an increasing trend with an estimatedlanding of 14,759 t in 2019. Kerala (41%) contributed the maximum followed by Tamil Nadu(28%), Andhra Pradesh (18%), Gujarat (8%) and Maharashtra (2%) to the total billfish landingsduring the last decade. Mechanised gillnetter cum hook and line was the major gear landingbillfishes. The major species landed during 2012-2019 were Istiophorus platypterus (52.2%),Istiompax indica (21.1%), Makaira nigricans (7.3%), Xiphias gladius (17.2%) and Kajikia audax(2.3%). Four of these species could be easily distinguished by COI barcodes but, the stripedmarlin, K. audax showed high sequence similarity with K. albida and cannot be distinguishedby barcodes alone. Control region (889 bp) provided a better phylogenetic signal, consistentwith that of the whole mitochondrial genome topology. The stock status plots of billfishesdepicted that, all the species were in the developing and exploited phase. Growth, mortalityand exploitation rates estimated for four billfishes indicated that the present fishing ratesand biomass levels are at safe levels and there is considerable scope for enhancing theirfishery. Keywords:CO1 barcodes, Exploitation rate, Large pelagics,Life history, Stock status plots","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.131133-02
Shubhadeep Ghosh, P. Rohit, E. M. Abdussamad, U. Ganga, M. R. Margaret, S. Surya, H. M. Manas
Keywords: Catch rates, Exploitation, Growth, India, Katsuwonus pelamis, Landings, Mortality
关键词捕获率 开发 生长 印度 海鲈 上岸量 死亡率
{"title":"Population parameters and stock status of skipjack tuna Katsuwonus pelamis (Linnaeus, 1758) from Indian waters","authors":"Shubhadeep Ghosh, P. Rohit, E. M. Abdussamad, U. Ganga, M. R. Margaret, S. Surya, H. M. Manas","doi":"10.21077/ijf.2023.70.3.131133-02","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.131133-02","url":null,"abstract":"Keywords: Catch rates, Exploitation, Growth, India, Katsuwonus pelamis, Landings, Mortality","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.129795-13
Shirsak Mondal, A. Deepika, Sagar Hundare, Nalini Poojary, T. J. Abraham, K. Sreedharan, G. Tripathi, K. V. Rajendran
Enterocytozoon hepatopenaei (EHP) is a serious pathogen impacting farmed shrimpproduction in Asian countries. Though many aquatic organisms were reported to besusceptible to EHP, the full range of susceptible hosts, which have the potential to transmitthe pathogen to shrimp has not been identified. In this study, a range of farmed and wildinvertebrates and other cohabiting fauna from different ecological niches located in thecoastal and inland saline areas were subjected to PCR-based detection employing primerstargeting three different genes coding for small subunit (ssu) rRNA, spore wall protein(SWP) and β-tubulin. The PCR analysis with ssu-rRNA primers showed positive amplificationin Penaeus vannamei, Penaeus monodon, Penaeus indicus, marine shrimps, aquatic insect,mud crabs, freshwater crab and violet clam. PCR using SWP primers detected EHP inP. vannamei, P. monodon, aquatic insect and marine crabs, while the β-tubulin primersresulted in positive amplification only in mud crabs and P. vannamei. The PCR ampliconsrevealed 99-100% identity with the sequences of EHP. The present study forms the firstattempt to screen a wide range of aquatic fauna for EHP employing three different PCR testsand the findings are significant as these organisms have the potential to be carriers of EHPand are likely to transmit the parasite to shrimp culture systems. Keywords:Co-habiting aquafauna, Enterocytozoonhepatopenaei, EHP, Host range, Shrimp culture
{"title":"A study on the natural host range of Enterocytozoon hepatopenaei in different species of shrimp and co-habiting aquatic fauna","authors":"Shirsak Mondal, A. Deepika, Sagar Hundare, Nalini Poojary, T. J. Abraham, K. Sreedharan, G. Tripathi, K. V. Rajendran","doi":"10.21077/ijf.2023.70.3.129795-13","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.129795-13","url":null,"abstract":"Enterocytozoon hepatopenaei (EHP) is a serious pathogen impacting farmed shrimpproduction in Asian countries. Though many aquatic organisms were reported to besusceptible to EHP, the full range of susceptible hosts, which have the potential to transmitthe pathogen to shrimp has not been identified. In this study, a range of farmed and wildinvertebrates and other cohabiting fauna from different ecological niches located in thecoastal and inland saline areas were subjected to PCR-based detection employing primerstargeting three different genes coding for small subunit (ssu) rRNA, spore wall protein(SWP) and β-tubulin. The PCR analysis with ssu-rRNA primers showed positive amplificationin Penaeus vannamei, Penaeus monodon, Penaeus indicus, marine shrimps, aquatic insect,mud crabs, freshwater crab and violet clam. PCR using SWP primers detected EHP inP. vannamei, P. monodon, aquatic insect and marine crabs, while the β-tubulin primersresulted in positive amplification only in mud crabs and P. vannamei. The PCR ampliconsrevealed 99-100% identity with the sequences of EHP. The present study forms the firstattempt to screen a wide range of aquatic fauna for EHP employing three different PCR testsand the findings are significant as these organisms have the potential to be carriers of EHPand are likely to transmit the parasite to shrimp culture systems. Keywords:Co-habiting aquafauna, Enterocytozoonhepatopenaei, EHP, Host range, Shrimp culture","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139332472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-30DOI: 10.21077/ijf.2023.70.3.132524-14
R. K. Brahmchari, Pushpa Kumari, Saurav Kumar, Rupam Sharma, K. Pani Prasad, P. P. Srivastava, R. Raman
Argulus, an ectoparasite of fish which is ubiquitous in wild and culture ponds, poses a majorchallenge with severe economic losses to the global aquaculture industry. With the increasein intensification of aquaculture practices, there is a dire need to discover new therapeuticoptions in the treatment of argulosis owing to the limited effectiveness of existing drugsand chemicals and their significant side effects. The use of metal nanoparticles hasshown promising results in the management of several parasitic infections. In this prelude,antiparasitic effect of biosynthesised iron nanoparticles was evaluated against Argulussiamensis under in vitro condition. Iron nanoparticles (FeNPs) were biosynthesised usingfresh Bauhinia racemosa leaf extract as a reducing agent and were characterised usingUV-VIS spectrophotometry, dynamic light scattering (DLS) technique, zeta potentialmeasurements, transmission electron microscopy (TEM), scanning electron microscopy(SEM), and fourier transform-infrared spectroscopy (FT-IR). For estimating antiparasiticefficacy of FeNPs under in vitro test, ten adult and juvenile parasites each were challengedfor 6 h separately in 20 ml of five different concentrations of FeNPs test solutions viz. 1.00,1.25, 1.50, 1.75 and 2.00 mg ml-1 in triplicate along with control groups for adults whereas,for juveniles, it was 0.75, 1.00, 1.25, 1.50 and 1.75 mg ml-1. Formation of FeNPs wasmeasured in 370-400 nm UV range. DLS showed an average FeNPs particle size of 119.8 nmwith a polydispersity index of 0.311. Zeta potential measurements showed negative surfacecharges (-11.3 mV) whereas, SEM and TEM micrographs revealed synthesised nanoparticleswere nearly spherical and size ranged from 60-270 nm. Further, FT-IR spectrum showed thepresence of Fe-O, N=O and O-H groups. Argulocidal effectiveness in both cases was foundto be concentration-dependent. The highest argulocidal activity of FeNPs was observed atconcentration of 1.75 mg ml-1 for juveniles and 2.00 mg ml-1 for adult argulids which led to100 and 87% mortality, respectively, in 6 h, however no mortality was recorded in controlgroup up to 16 h. Furthermore, the calculated 6 h-EC50 of biosynthesised FeNPs for juvenileand adult argulid parasites was determined as 0.97 and 1.27 mg ml-1. Results of the presentstudy showed that short term bath treatment with biosynthesised FeNPs is effective againstargulid parasites. However, further research is required to evaluate its therapeutic potentialunder in vivo condition. Keywords:Argulosis, Argulus siamensis, Iron nanoparticles,Parasiticidal activity
{"title":"Antiparasitic effect of biogenic iron nanoparticles against the fish ectoparasite Argulus siamensis: In vitro study","authors":"R. K. Brahmchari, Pushpa Kumari, Saurav Kumar, Rupam Sharma, K. Pani Prasad, P. P. Srivastava, R. Raman","doi":"10.21077/ijf.2023.70.3.132524-14","DOIUrl":"https://doi.org/10.21077/ijf.2023.70.3.132524-14","url":null,"abstract":"Argulus, an ectoparasite of fish which is ubiquitous in wild and culture ponds, poses a majorchallenge with severe economic losses to the global aquaculture industry. With the increasein intensification of aquaculture practices, there is a dire need to discover new therapeuticoptions in the treatment of argulosis owing to the limited effectiveness of existing drugsand chemicals and their significant side effects. The use of metal nanoparticles hasshown promising results in the management of several parasitic infections. In this prelude,antiparasitic effect of biosynthesised iron nanoparticles was evaluated against Argulussiamensis under in vitro condition. Iron nanoparticles (FeNPs) were biosynthesised usingfresh Bauhinia racemosa leaf extract as a reducing agent and were characterised usingUV-VIS spectrophotometry, dynamic light scattering (DLS) technique, zeta potentialmeasurements, transmission electron microscopy (TEM), scanning electron microscopy(SEM), and fourier transform-infrared spectroscopy (FT-IR). For estimating antiparasiticefficacy of FeNPs under in vitro test, ten adult and juvenile parasites each were challengedfor 6 h separately in 20 ml of five different concentrations of FeNPs test solutions viz. 1.00,1.25, 1.50, 1.75 and 2.00 mg ml-1 in triplicate along with control groups for adults whereas,for juveniles, it was 0.75, 1.00, 1.25, 1.50 and 1.75 mg ml-1. Formation of FeNPs wasmeasured in 370-400 nm UV range. DLS showed an average FeNPs particle size of 119.8 nmwith a polydispersity index of 0.311. Zeta potential measurements showed negative surfacecharges (-11.3 mV) whereas, SEM and TEM micrographs revealed synthesised nanoparticleswere nearly spherical and size ranged from 60-270 nm. Further, FT-IR spectrum showed thepresence of Fe-O, N=O and O-H groups. Argulocidal effectiveness in both cases was foundto be concentration-dependent. The highest argulocidal activity of FeNPs was observed atconcentration of 1.75 mg ml-1 for juveniles and 2.00 mg ml-1 for adult argulids which led to100 and 87% mortality, respectively, in 6 h, however no mortality was recorded in controlgroup up to 16 h. Furthermore, the calculated 6 h-EC50 of biosynthesised FeNPs for juvenileand adult argulid parasites was determined as 0.97 and 1.27 mg ml-1. Results of the presentstudy showed that short term bath treatment with biosynthesised FeNPs is effective againstargulid parasites. However, further research is required to evaluate its therapeutic potentialunder in vivo condition. Keywords:Argulosis, Argulus siamensis, Iron nanoparticles,Parasiticidal activity","PeriodicalId":50372,"journal":{"name":"Indian Journal of Fisheries","volume":null,"pages":null},"PeriodicalIF":0.5,"publicationDate":"2023-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139333314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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