Enzymatic biodegradation and detoxification of azo and anthraquinone dyes by indigenously isolated bacterial monocultures and their synergistic behaviour in developed consortia

IF 1.7 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Water and Environment Journal Pub Date : 2023-09-20 DOI:10.1111/wej.12903
Khalid Hussain, Afsheen Aman, Asad Ullah, Muhammad Qamar Khan, Asma Ansari
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Abstract

Abstract Wastewater generated by textile industry needs to be treated to reduce its toxicity before final disposal and/or for recycling purposes. In the current study, several bacterial strains were screened for dye decolorization potential. UV–visible spectroscopy was used to determine maximum absorption wavelength of disperse dyes. HPLC and MTS assay were used to confirm the degradation and detoxification of disperse dyes, respectively. Results revealed that indigenously isolated Bacillus licheniformis , Glutamicibacter uratoxydans and Pseudomonas aeruginosa showed strong decolorization of red, blue and violet, respectively in 6–9 h. MTS assay revealed 100% viability of NIH/3T3 cell lines in presence of treated dyes. Enzyme screening assay confirmed the production of intracellular and membrane bound oxidoreductases in presence of specific dye as substrate. To resolve this issue, bacterial consortia were prepared, and better decolorization of all dyes was achieved in synergistic behaviour of Consortia 1 and 4 with 85% and 88% decolorization potential, respectively.
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偶氮和蒽醌染料在本土分离细菌单一培养中的酶降解和解毒作用及其在发达联合体中的协同作用
纺织工业产生的废水在最终处置和/或回收利用之前需要进行处理以降低其毒性。在本研究中,筛选了几种菌株的染料脱色潜力。采用紫外可见光谱法测定分散染料的最大吸收波长。采用高效液相色谱法和MTS法分别对分散染料进行降解和解毒鉴定。结果表明,本土分离的地衣芽孢杆菌、脲氧基谷氨酰胺杆菌和铜绿假单胞菌在6-9 h内分别表现出较强的红色、蓝色和紫色脱色效果。MTS实验显示,处理后的染料对NIH/3T3细胞株的存活率为100%。酶筛选实验证实,在特定染料作为底物存在的情况下,细胞内和膜结合氧化还原酶的产生。为了解决这一问题,制备了细菌联合体,在联合体1和联合体4的协同作用下,所有染料的脱色效果都较好,脱色电位分别为85%和88%。
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来源期刊
Water and Environment Journal
Water and Environment Journal 环境科学-湖沼学
CiteScore
4.80
自引率
0.00%
发文量
67
审稿时长
18-36 weeks
期刊介绍: Water and Environment Journal is an internationally recognised peer reviewed Journal for the dissemination of innovations and solutions focussed on enhancing water management best practice. Water and Environment Journal is available to over 12,000 institutions with a further 7,000 copies physically distributed to the Chartered Institution of Water and Environmental Management (CIWEM) membership, comprised of environment sector professionals based across the value chain (utilities, consultancy, technology suppliers, regulators, government and NGOs). As such, the journal provides a conduit between academics and practitioners. We therefore particularly encourage contributions focussed at the interface between academia and industry, which deliver industrially impactful applied research underpinned by scientific evidence. We are keen to attract papers on a broad range of subjects including: -Water and wastewater treatment for agricultural, municipal and industrial applications -Sludge treatment including processing, storage and management -Water recycling -Urban and stormwater management -Integrated water management strategies -Water infrastructure and distribution -Climate change mitigation including management of impacts on agriculture, urban areas and infrastructure
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