Regulation of T16H subcellular localization for promoting its catalytic efficiency in yeast cells.

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Biotechnology Letters Pub Date : 2024-02-01 Epub Date: 2023-11-16 DOI:10.1007/s10529-023-03442-3

Jiancong Cao, Bingrun Yang, Mengxia Zhang, Fang Yu

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Abstract

To investigate the effect of subcellular localization on the transformation efficiency of heterologous expressed functional P450s in yeast. Microbial biotransformation offers a promising substitute for the direct extraction of natural products, but its viability in industrial applications depends on achieving high transformation efficiencies. To investigate the influence of subcellular microenvironments on the activity of heterologously expressed P450s, Catharanthus roseus tabersonine 16-hydroxylase (T16H) was chosen, and its subcellular localization was regulated by fusing organelle-localization signals. Interestingly, this manipulation had no effect on the gene expression levels of T16H, but resulted in varying conversion rates from tabersonine to 16-hydroxy tabersonine. Notably, the highest transformation efficiency was observed in yeast cells expressing peroxisome-localized T16H. Given the alkaline pH optimum for P450s, the alkaline peroxisomal lumen could be a suitable compartment for P450s reactions to achieve high transformation efficiency using yeast cells. Different organelle-localization of T16H in yeast cells resulted in varying conversion rates, suggesting that compartmentalizing the expression of target enzymes could be a viable approach to increase transformation efficiency in yeast.

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调控T16H亚细胞定位促进其在酵母细胞中的催化效率。

目的研究亚细胞定位对外源表达功能p450在酵母中转化效率的影响。微生物转化为直接提取天然产物提供了一个有前途的替代品，但其在工业应用中的可行性取决于实现高转化效率。为了研究亚细胞微环境对P450s异源表达活性的影响，我们选择了花蔷薇tabersonine 16-羟化酶(T16H)，并通过融合细胞器定位信号调控其亚细胞定位。有趣的是，这种操作对T16H的基因表达水平没有影响，但导致从tabersonine到16-羟基tabersonine的转化率不同。值得注意的是，在表达过氧化物酶体定位的T16H的酵母细胞中观察到最高的转化效率。考虑到p450的最佳碱性pH，碱性过氧化物酶体管腔可以作为p450反应的合适腔室，以实现酵母细胞的高转化效率。酵母细胞中不同的T16H细胞器定位导致了不同的转化率，表明区隔化靶酶的表达可能是一种提高酵母转化效率的可行方法。

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来源期刊

Biotechnology Letters 工程技术-生物工程与应用微生物

CiteScore

5.90

自引率

3.70%

发文量

108

审稿时长

1.2 months

期刊介绍： Biotechnology Letters is the world’s leading rapid-publication primary journal dedicated to biotechnology as a whole – that is to topics relating to actual or potential applications of biological reactions affected by microbial, plant or animal cells and biocatalysts derived from them. All relevant aspects of molecular biology, genetics and cell biochemistry, of process and reactor design, of pre- and post-treatment steps, and of manufacturing or service operations are therefore included. Contributions from industrial and academic laboratories are equally welcome. We also welcome contributions covering biotechnological aspects of regenerative medicine and biomaterials and also cancer biotechnology. Criteria for the acceptance of papers relate to our aim of publishing useful and informative results that will be of value to other workers in related fields. The emphasis is very much on novelty and immediacy in order to justify rapid publication of authors’ results. It should be noted, however, that we do not normally publish papers (but this is not absolute) that deal with unidentified consortia of microorganisms (e.g. as in activated sludge) as these results may not be easily reproducible in other laboratories. Papers describing the isolation and identification of microorganisms are not regarded as appropriate but such information can be appended as supporting information to a paper. Papers dealing with simple process development are usually considered to lack sufficient novelty or interest to warrant publication.