Establishment of a reference interval for thiamine concentrations in healthy dogs and evaluation of the prevalence of absolute thiamine deficiency in critically ill dogs with and without sepsis using high-performance liquid chromatography

IF 1.1 3区 农林科学 Q3 VETERINARY SCIENCES Journal of veterinary emergency and critical care Pub Date : 2023-11-21 DOI:10.1111/vec.13341
Noa Berlin DVM, DACVECC, Alexandra Pfaff Med Vet, DACVECC, Elizabeth A. Rozanski DVM, DACVECC, DACVIM, Nolan V. Chalifoux DVM, DACVECC, Rebecka S. Hess DVM, MSCE, DACVIM, Michael W. Donnino MD, Deborah C. Silverstein DVM, DACVECC
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Abstract

Objective

To determine the normal reference interval (RI) for thiamine concentrations in healthy dogs and investigate the prevalence of thiamine deficiency in critically ill dogs with and without sepsis.

Design

Prospective, observational, multicenter study, conducted between 2019 and 2021.

Setting

Two veterinary university teaching hospitals.

Animals

A total of 109 dogs were enrolled into 3 groups: 40 healthy dogs, 33 dogs with suspected or confirmed sepsis and evidence of tissue hypoperfusion (Doppler blood pressure ≤90 mm Hg or plasma lactate ≥3 mmol/L), and 36 dogs with other critical illnesses and evidence of tissue hypoperfusion.

Interventions

For each dog, CBC, serum biochemistry, plasma lactate concentration, whole-blood thiamine concentration, blood pressure, vital parameters, Acute Patient Physiologic and Laboratory Evaluation (APPLE)fast score, and clinical outcomes were recorded, alongside basic patient parameters and dietary history. Whole-blood thiamine pyrophosphate (TPP) concentrations were measured using high-performance liquid chromatography.

Measurements and Main Results

The RI for whole-blood TPP in healthy dogs was 70.9–135.3 μg/L. Median TPP concentrations were significantly lower in septic dogs compared to healthy controls (P = 0.036). No significant difference in median TPP concentrations was found between septic dogs and nonseptic critically ill dogs, or between healthy dogs and nonseptic critically ill dogs. TPP concentrations were below the normal RI in 27.3% of septic dogs, compared to 19.4% of nonseptic critically ill dogs (P = 0.57). No correlations were found between TPP concentrations and lactate concentrations, age, body condition scores, time since last meal, RBC count, serum alanine aminotransferase, APPLEfast scores, or patient outcomes.

Conclusions

TPP concentrations were significantly lower in septic dogs compared to healthy controls, with an absolute thiamine deficiency found in 27.3% of septic dogs. The established TPP RI allows for further investigation of thiamine deficiency in critically ill dogs.

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建立健康犬体内硫胺素浓度的参考区间,并利用高效液相色谱法评估伴有和不伴有败血症的危重犬体内绝对硫胺素缺乏症的患病率。
目的:确定健康犬体内硫胺素浓度的正常参考区间(RI),探讨伴有和不伴有败血症的危重犬体内硫胺素缺乏症的流行情况。设计:前瞻性、观察性、多中心研究,于2019年至2021年进行。单位:两所兽医大学教学医院。动物:109只犬分为3组:健康犬40只,疑似或确诊脓毒症且存在组织灌注不足(多普勒血压≤90 mm Hg或血浆乳酸≥3 mmol/L)犬33只,其他危重疾病且存在组织灌注不足的犬36只。干预措施:记录每只狗的CBC、血清生化、血浆乳酸浓度、全血硫胺素浓度、血压、生命参数、急性患者生理和实验室评估(APPLE)快速评分和临床结果,以及患者基本参数和饮食史。采用高效液相色谱法测定全血焦磷酸硫胺素(TPP)浓度。测量结果及主要结果:健康犬全血TPP的RI为70.9 ~ 135.3 μg/L。与健康对照相比,脓毒犬中位TPP浓度显著降低(P = 0.036)。在脓毒症犬与非脓毒症危重犬、健康犬与非脓毒症危重犬之间,TPP中位浓度无显著差异。27.3%的脓毒症犬的TPP浓度低于正常RI,而19.4%的非脓毒症危重犬的TPP浓度低于正常RI (P = 0.57)。TPP浓度与乳酸浓度、年龄、身体状况评分、最后一餐后时间、红细胞计数、血清丙氨酸转氨酶、APPLEfast评分或患者预后之间没有相关性。结论:与健康对照相比,败血症犬的TPP浓度显著降低,27.3%的败血症犬存在绝对硫胺素缺乏症。已建立的跨太平洋伙伴关系研究所允许对危重犬的硫胺素缺乏症进行进一步调查。
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来源期刊
CiteScore
2.50
自引率
15.40%
发文量
121
审稿时长
18-36 weeks
期刊介绍: The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.
期刊最新文献
Fabio Viganó Jennifer J. Devey Issue Information - Prelim AUTHOR INDEX Abstracts from the International Veterinary Emergency and Critical Care Symposium and the European Veterinary Emergency and Critical Care Annual Congress 2024
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