Development and Application of Prime Editing in Plants

IF 5.6 2区农林科学 Q1 AGRONOMY Rice Science Pub Date : 2023-11-01 DOI:10.1016/j.rsci.2023.07.005

Liu Tingting , Zou Jinpeng , Yang Xi , Wang Kejian , Rao Yuchun , Wang Chun

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Abstract

Clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated protein (Cas)-mediated genome editing has greatly accelerated progress in plant genetic research and agricultural breeding by enabling targeted genomic modifications. Moreover, the prime editing system, derived from the CRISPR/Cas system, has opened the door for even more precise genome editing. Prime editing has the capability to facilitate all 12 types of base-to-base conversions, as well as desired insertions or deletions of fragments, without inducing double-strand breaks and requiring donor DNA templet. In a short time, prime editing has been rapidly verified as functional in various plants, and can be used in plant genome functional analysis as well as precision breeding of crops. In this review, we summarize the emergence and development of prime editing, highlight recent advances in improving its efficiency in plants, introduce the current applications of prime editing in plants, and look forward to future prospects for utilizing prime editing in genetic improvement and precision molecular breeding.

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植物引体编辑技术的发展与应用

聚集规律间隔回文重复序列(CRISPR)/CRISPR相关蛋白(Cas)介导的基因组编辑通过实现靶向基因组修饰，极大地加快了植物遗传研究和农业育种的进展。此外，源自CRISPR/Cas系统的初始编辑系统为更精确的基因组编辑打开了大门。启动编辑能够促进所有12种碱基到碱基的转换，以及所需的片段插入或删除，而不诱导双链断裂和需要供体DNA模板。在短时间内，引体编辑在多种植物中迅速得到验证，可用于植物基因组功能分析和作物的精准育种。本文综述了引体编辑技术的产生和发展，重点介绍了引体编辑技术在提高植物效率方面的最新进展，介绍了引体编辑技术在植物中的应用现状，并展望了引体编辑技术在遗传改良和精密分子育种中的应用前景。

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来源期刊

Rice Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

8.90

自引率

6.20%

发文量

审稿时长

40 weeks

期刊介绍： Rice Science is an international research journal sponsored by China National Rice Research Institute. It publishes original research papers, review articles, as well as short communications on all aspects of rice sciences in English language. Some of the topics that may be included in each issue are: breeding and genetics, biotechnology, germplasm resources, crop management, pest management, physiology, soil and fertilizer management, ecology, cereal chemistry and post-harvest processing.