Immunoinformatics analysis of Brucella melitensis to approach a suitable vaccine against brucellosis.

IF 3.6 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Journal, genetic engineering & biotechnology Pub Date : 2023-11-29 DOI:10.1186/s43141-023-00614-6
Pejman Hashemzadeh, Saba Asgari Nezhad, Hossein Khoshkhabar
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Abstract

Background: Brucellosis caused by B. melitensis is one of the most important common diseases between humans and livestock. Currently, live attenuated vaccines are used for this disease, which causes many problems, and unfortunately, there is no effective vaccine for human brucellosis. The aim of our research was to design a recombinant vaccine containing potential immunogenic epitopes against B. melitensis.

Methods: In this study, using immunoinformatics approaches, 3 antigens Omp31, Omp25, and Omp28 were identified and the amino acid sequence of the selected antigens was determined in NCBI. Signal peptides were predicted by SignaIP-5.0 server. To predict B-cell epitopes from ABCpred and Bcepred servers, to predict MHC-I epitopes from RANKPEP and SYFPEITHI servers, to predict MHC-II epitopes from RANKPEP and MHCPred servers, and to predict CTL epitopes were used from the CTLPred server. Potentially immunogenic final epitopes were joined by flexible linkers. Finally, allergenicity (AllerTOP 2.0 server), antigenicity (Vaxijen server), physicochemical properties (ProtParam server), solubility (Protein-sol server), secondary (PSIPRED and GRO4 servers) and tertiary structure (I-TASSER server), refinement (GalaxyWEB server), validation (ProSA-web, Molprobity, and ERRAT servers), and optimization of the codon sequence (JCat server) of the structure of the multi-epitope vaccine were analyzed.

Results: The analysis of immunoinformatics tools showed that the designed vaccine has high quality, acceptable physicochemical properties, and can induce humoral and cellular immune responses against B. melitensis bacteria. In addition, the high expression level of recombinant antigens in the E. coli host was observed through in silico simulation.

Conclusion: According to the results in silico, the designed vaccine can be a suitable candidate to fight brucellosis and in vitro and in vivo studies are needed to evaluate the research of this study.

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对梅利特布氏菌的免疫信息学分析探讨合适的布氏菌病疫苗。
背景:由羊分枝杆菌引起的布鲁氏菌病是人类与牲畜之间最重要的常见疾病之一。目前,这种疾病使用减毒活疫苗,这导致了许多问题,不幸的是,没有有效的人类布鲁氏菌病疫苗。我们的研究目的是设计一种含有潜在免疫原性表位的重组疫苗。方法:采用免疫信息学方法对NCBI中3种抗原Omp31、Omp25和Omp28进行鉴定,并测定所选抗原的氨基酸序列。信号肽预测采用SignaIP-5.0服务器。从ABCpred和Bcepred服务器预测b细胞表位,从RANKPEP和SYFPEITHI服务器预测MHC-I表位,从RANKPEP和MHCPred服务器预测MHC-II表位,并从CTLPred服务器预测CTL表位。潜在的免疫原性最终表位由柔性连接体连接。最后,对多表位疫苗结构的致敏性(AllerTOP 2.0服务器)、抗原性(Vaxijen服务器)、理化性质(ProtParam服务器)、溶解度(Protein-sol服务器)、二级(PSIPRED和GRO4服务器)和三级结构(I-TASSER服务器)、精化(GalaxyWEB服务器)、验证(ProSA-web、Molprobity和ERRAT服务器)和密码子序列优化(JCat服务器)进行分析。结果:免疫信息学工具分析表明,所设计的疫苗具有高质量、可接受的物理化学性质,可诱导针对梅利氏杆菌的体液和细胞免疫应答。此外,通过计算机模拟观察到重组抗原在大肠杆菌宿主体内的高表达水平。结论:根据硅片实验结果,所设计的疫苗可作为抗布鲁氏菌病的合适候选疫苗,需要进行体外和体内研究来评价本研究的研究成果。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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