Molecular detection of Babesia vesperuginis in bats from Lithuania

IF 3.1 2区医学 Q2 INFECTIOUS DISEASES Ticks and Tick-borne Diseases Pub Date : 2023-11-28 DOI:10.1016/j.ttbdis.2023.102283

Povilas Sakalauskas , Evelina Kaminskienė , Dovilė Bukauskaitė , Vytautas Eigirdas , Justina Snegiriovaitė , Dalytė Mardosaitė-Busaitienė , Algimantas Paulauskas

{"title":"Molecular detection of Babesia vesperuginis in bats from Lithuania","authors":"Povilas Sakalauskas , Evelina Kaminskienė , Dovilė Bukauskaitė , Vytautas Eigirdas , Justina Snegiriovaitė , Dalytė Mardosaitė-Busaitienė , Algimantas Paulauskas","doi":"10.1016/j.ttbdis.2023.102283","DOIUrl":null,"url":null,"abstract":"<div>Babesia vesperuginis is an intraerythrocytic protozoan parasite that circulates among bats and ticks in many countries worldwide. However, the distribution of B. vesperuginis in the Baltic region has not been studied. A total of 86 dead bats from eight different species were collected and screened for Babesia spp. using real-time PCR. Overall, 52.3% (45/86) of the bats were found positive for Babesia spp. The prevalence of Babesia spp. in different organs varied, with the highest prevalence observed in heart tissues (37.0%) and the lowest in liver tissues (22.2%). However, the observed differences in prevalence among organs were not statistically significant. Blood samples from 125 bats of nine different species were also analyzed for Babesia spp. prevalence using real-time PCR and nested PCR. The results showed a prevalence of 35.2% and 22.4%, respectively. Moreover, 28.3% (17/60) of the examined blood samples were confirmed positive for Babesia spp. through blood smear analysis. The total of 32 partial sequences of the 18S rRNA gene derived in this study were 100% identical to B. vesperuginis sequences from GenBank. In eight species of bats, Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Vespertilio murinus, Eptesicus nilssonii, Eptesicus serotinus, Myotis daubentonii and Nyctalus noctula, Babesia parasites were identified. In E. nilssonii, Babesia spp. was identified for the first time.</div>","PeriodicalId":49320,"journal":{"name":"Ticks and Tick-borne Diseases","volume":"15 1","pages":"Article 102283"},"PeriodicalIF":3.1000,"publicationDate":"2023-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1877959X23001644/pdfft?md5=725ac442a54f4e4593358c87194c2123&pid=1-s2.0-S1877959X23001644-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ticks and Tick-borne Diseases","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1877959X23001644","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INFECTIOUS DISEASES","Score":null,"Total":0}

引用次数: 0

Abstract

Babesia vesperuginis is an intraerythrocytic protozoan parasite that circulates among bats and ticks in many countries worldwide. However, the distribution of B. vesperuginis in the Baltic region has not been studied. A total of 86 dead bats from eight different species were collected and screened for Babesia spp. using real-time PCR. Overall, 52.3% (45/86) of the bats were found positive for Babesia spp. The prevalence of Babesia spp. in different organs varied, with the highest prevalence observed in heart tissues (37.0%) and the lowest in liver tissues (22.2%). However, the observed differences in prevalence among organs were not statistically significant. Blood samples from 125 bats of nine different species were also analyzed for Babesia spp. prevalence using real-time PCR and nested PCR. The results showed a prevalence of 35.2% and 22.4%, respectively. Moreover, 28.3% (17/60) of the examined blood samples were confirmed positive for Babesia spp. through blood smear analysis. The total of 32 partial sequences of the 18S rRNA gene derived in this study were 100% identical to B. vesperuginis sequences from GenBank. In eight species of bats, Pipistrellus nathusii, Pipistrellus pipistrellus, Pipistrellus pygmaeus, Vespertilio murinus, Eptesicus nilssonii, Eptesicus serotinus, Myotis daubentonii and Nyctalus noctula, Babesia parasites were identified. In E. nilssonii, Babesia spp. was identified for the first time.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

立陶宛蝙蝠巴贝斯虫的分子检测

巴贝斯虫是一种红细胞内原生动物寄生虫，在世界上许多国家的蝙蝠和蜱中传播。然而，对其在波罗的海地区的分布尚未进行研究。收集8个不同种类的86只蝙蝠尸体，采用实时荧光定量PCR技术对巴贝斯虫进行检测。总体上，52.3%(45/86)的蝙蝠巴贝斯虫呈阳性，不同器官的巴贝斯虫感染率不同，心脏组织最高(37.0%)，肝脏组织最低(22.2%)。然而，观察到的各器官患病率差异无统计学意义。采用实时荧光定量PCR和巢式荧光定量PCR对9种125只蝙蝠的血样进行巴贝斯虫流行率分析。结果显示患病率分别为35.2%和22.4%。此外，28.3%(17/60)的血样经血液涂片分析呈巴贝斯虫阳性。本研究获得的18S rRNA基因的32个部分序列与GenBank中的B. vesperuginis序列完全相同。在nathusii Pipistrellus、Pipistrellus、pygmaeus、Vespertilio murinus、nilsonii Eptesicus、serotinus Eptesicus、daubentonmyotis和Nyctalus noctula 8种蝙蝠中鉴定出巴贝斯虫。在尼罗氏伊蚊中首次检出巴贝斯虫。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Ticks and Tick-borne Diseases INFECTIOUS DISEASES-MICROBIOLOGY

CiteScore

6.90

自引率

12.50%

发文量

185

审稿时长

6-12 weeks

期刊介绍： Ticks and Tick-borne Diseases is an international, peer-reviewed scientific journal. It publishes original research papers, short communications, state-of-the-art mini-reviews, letters to the editor, clinical-case studies, announcements of pertinent international meetings, and editorials. The journal covers a broad spectrum and brings together various disciplines, for example, zoology, microbiology, molecular biology, genetics, mathematical modelling, veterinary and human medicine. Multidisciplinary approaches and the use of conventional and novel methods/methodologies (in the field and in the laboratory) are crucial for deeper understanding of the natural processes and human behaviour/activities that result in human or animal diseases and in economic effects of ticks and tick-borne pathogens. Such understanding is essential for management of tick populations and tick-borne diseases in an effective and environmentally acceptable manner.