Adipose tissue-derived mesenchymal stromal cells attenuate acute lung injury induced by trauma and haemorrhagic shock

IF 2.5 4区医学 Q3 IMMUNOLOGY Immunobiology Pub Date : 2023-11-01 DOI:10.1016/j.imbio.2023.152765

Zhi Zhang , Xiaoxia Yang , Qinghong Meng , Yiyin Long , Xiaofeng Shi , Yuliang Wang

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Abstract

Background

Mesenchymal stromal cells (MSCs) have shown promising therapeutic options for acute lung injury (ALI) caused by multiple factors. Here, we evaluated the therapeutic potential of adipose tissue-derived mesenchymal stromal cells (ADSCs) in trauma and hemorrhagic shock (THS)-induced ALI.

Methods

ALI model induced by THS was constructed by fractures plus abdominal trauma plus acute hemorrhage plus fluid resuscitation. The ADSCs group rats were generated by injecting 2 × 10⁶ ADSCs at 0 and 1 h after THS. The sham, ALI, and ADSCs group rats were sacrificed at 24 h after resuscitation. The changes in lung histopathology, total protein in bronchoalveolar lavage fluid (BALF), mRNA expression of pro-inflammatory/anti-inflammatory cytokines, antioxidant, and anti-apoptotic indicator, and the activity of Toll-like receptor 4 (TLR4) signaling in lung tissues were evaluated.

Results

Administration of the ADSCs reversed ALI induced by THS, including lung histopathological changes/scores, and BALF total protein concentration. Additionally, ADSCs therapy also significantly down-regulated mRNA expression of pro-inflammatory TNF-α, IL-1β, and IL-6, up-regulated mRNA expression of anti-inflammatory IL-10, anti-apoptotic molecule Bcl-2, and anti-oxidative molecule HO-1 in THS rats. Furthermore, ADSCs suppressed the expression of TLR4 in lung tissue.

Conclusion

Our data show that ADSCs administration can exert therapeutic effects on THS-induced ALI in rats and may provide beneficial in preventative strategies for ALI.

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脂肪组织来源的间充质间质细胞可减轻外伤和出血性休克引起的急性肺损伤

间充质间质细胞(MSCs)在多种因素引起的急性肺损伤(ALI)中显示出有希望的治疗选择。在这里，我们评估了脂肪组织来源的间充质间质细胞(ADSCs)在创伤和失血性休克(THS)诱导的ALI中的治疗潜力。方法采用骨折+腹部外伤+急性出血+液体复苏的方法，建立三手烟致sali模型。注射2 × 106个ADSCs，分别于三手通气后0、1 h生成ADSCs组大鼠。假手术组、ALI组和ADSCs组大鼠复苏后24 h处死。观察大鼠肺组织病理学、支气管肺泡灌洗液(BALF)总蛋白、促炎/抗炎细胞因子、抗氧化、抗凋亡指标mRNA表达及肺组织toll样受体4 (TLR4)信号活性的变化。结果ADSCs可逆转三手烟诱导的ALI，包括肺组织病理学改变/评分和BALF总蛋白浓度。此外，ADSCs治疗还显著下调THS大鼠促炎TNF-α、IL-1β、IL-6 mRNA表达，上调抗炎IL-10、抗凋亡分子Bcl-2、抗氧化分子HO-1 mRNA表达。此外，ADSCs抑制TLR4在肺组织中的表达。结论给药ADSCs对诱导的大鼠ALI有一定的治疗作用，并可能为ALI的预防提供有益的策略。

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来源期刊

Immunobiology 医学-免疫学

CiteScore

5.00

自引率

3.60%

发文量

108

审稿时长

55 days

期刊介绍： Immunobiology is a peer-reviewed journal that publishes highly innovative research approaches for a wide range of immunological subjects, including • Innate Immunity, • Adaptive Immunity, • Complement Biology, • Macrophage and Dendritic Cell Biology, • Parasite Immunology, • Tumour Immunology, • Clinical Immunology, • Immunogenetics, • Immunotherapy and • Immunopathology of infectious, allergic and autoimmune disease.