Establishment of bladder cancer spheroids and cultured in microfluidic platform for predicting drug response

IF 6.1 2区 医学 Q1 ENGINEERING, BIOMEDICAL Bioengineering & Translational Medicine Pub Date : 2023-12-04 DOI:10.1002/btm2.10624
Qiao Xiong, Ting Liu, Yidie Ying, Xiaowen Yu, Ziwei Wang, Hongliang Gao, Tianhai Lin, Weihua Fan, Zhensheng Zhang, Qiang Wei, Yuqing Ge, Shuxiong Zeng, Chuanliang Xu
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Abstract

Cisplatin-containing combination chemotherapy has been used as the standard treatment for bladder cancer patients at advanced stage. However, nearly 50% of patients are nonresponders. To guide the selection of more effective chemotherapeutic agents, a bladder cancer spheroids microfluidic drug sensitivity analysis system was established in this study. Bladder cancer spheroids were established and successfully cultured in a customized microfluidic device to assess their response to different chemotherapeutic agents. The in vitro drug sensitivity results were also compared to patient-derived xenograft (PDX) models and clinical responses of patients. As a result, bladder cancer spheroids faithfully recapitulate the histopathological and genetic features of their corresponding parental tumors. Furthermore, the in vitro drug sensitivity outcomes of spheroids (n = 8) demonstrated a high level of correlation with the PDX (n = 2) and clinical response in patients (n = 2). Our study highlights the potential of combining bladder cancer spheroids and microfluidic devices as an efficient and accurate platform for personalized selection of chemotherapeutic agents.

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建立膀胱癌球体并在微流控平台中培养,以预测药物反应
含顺铂联合化疗已成为晚期膀胱癌患者的标准治疗方案。然而,近50%的患者无反应。为了指导更有效的化疗药物的选择,本研究建立了膀胱癌球体微流控药物敏感性分析系统。在定制的微流体装置中建立并成功培养膀胱癌球体,以评估其对不同化疗药物的反应。体外药敏结果也与患者来源的异种移植(PDX)模型和患者的临床反应进行了比较。因此,膀胱癌球状体忠实地概括了其相应亲代肿瘤的组织病理和遗传特征。此外,球体的体外药敏结果(n = 8)与患者的PDX (n = 2)和临床反应(n = 2)高度相关。我们的研究强调了将膀胱癌球体和微流控装置结合起来作为个性化选择化疗药物的有效和准确平台的潜力。
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来源期刊
Bioengineering & Translational Medicine
Bioengineering & Translational Medicine Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
8.40
自引率
4.10%
发文量
150
审稿时长
12 weeks
期刊介绍: Bioengineering & Translational Medicine, an official, peer-reviewed online open-access journal of the American Institute of Chemical Engineers (AIChE) and the Society for Biological Engineering (SBE), focuses on how chemical and biological engineering approaches drive innovative technologies and solutions that impact clinical practice and commercial healthcare products.
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